ID pDEST-EGFP standard; circular DNA; ; 6450 BP. DE LMBP 4542. CC Accession number: LMBP 4542 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 9-DEC-2010 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 1..568 FT /vntifkey="29" FT /label=hCMV-IE\promoter\and\enhancer FT CDS 613..1329 FT /vntifkey="4" FT /label=EGFP FT attenuator complement(1402..1526) FT /vntifkey="0" FT /label=attR1 FT CDS 1635..2294 FT /vntifkey="4" FT /label=chloramphenicol\resistance FT CDS 2636..2941 FT /vntifkey="4" FT /label=ccdB FT attenuator 2982..3106 FT /vntifkey="0" FT /label=attR2 FT rep_origin complement(3366..3821) FT /vntifkey="33" FT /label=f1\ori FT terminator 3216..3312 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(3216..3312) FT /vntifkey="43" FT /label=SV40\polyA\late FT promoter 3973..4254 FT /vntifkey="29" FT /label=SV40\early\promoter FT rep_origin 6118..6444 FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT RBS 3918..3952 FT /vntifkey="32" FT /label=5'\UTR\of\amp,\incl.\RBS FT CDS 4346..5140 FT /vntifkey="4" FT /label=Tn5\neomycin\resistance FT rep_origin 4211..4286 FT /vntifkey="33" FT /label=SV40\ori FT promoter 3822..3917 FT /vntifkey="30" FT /label=ampicillin\resistance\promoter FT promoter 1534..1586 FT /vntifkey="30" FT /label=lacUV5\promoter FT terminator 5331..5400 FT /vntifkey="43" FT /label=HSV-TK\polyA SQ Sequence 6450 BP; 1623 A; 1636 C; 1650 G; 1541 t; tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 60 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 120 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 180 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 240 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 300 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 360 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 420 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 480 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 540 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 600 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 660 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 720 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 780 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 840 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 900 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 960 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1020 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 1080 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 1140 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1200 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 1260 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1320 ctgtacaagt ccggccggac tcagatctcg agctcaagct tcgaattctg cagtcgacgg 1380 taccgcgggc ccgggatcat cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat 1440 gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt 1500 aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt 1560 atgcttccgg ctcgtataat gtgtggattt tgagttagga tccggcgaga ttttcaggag 1620 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat 1680 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga 1740 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt 1800 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg 1860 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc 1920 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt 1980 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta 2040 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt 2100 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat 2160 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgtct 2220 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc 2280 agggcggggc gtaaacgcgt ggatccggct tactaaaagc cagataacag tatgcgtatt 2340 tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa 2400 aaagaggtgt gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt 2460 gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa 2520 gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc 2580 gcccggttta ttgaaatgaa cggctctttt gctgacgaga acagggactg gtgaaatgca 2640 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag 2700 tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct 2760 gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg 2820 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga 2880 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata 2940 aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt 3000 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat 3060 ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtgatg atccaccgga 3120 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa 3180 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac 3240 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 3300 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa 3360 cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 3420 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 3480 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 3540 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 3600 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 3660 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 3720 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 3780 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg 3840 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 3900 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc 3960 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 4020 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 4080 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 4140 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 4200 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 4260 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca 4320 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc 4380 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc 4440 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga 4500 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac 4560 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct 4620 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa 4680 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc 4740 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct 4800 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc 4860 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg 4920 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct 4980 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct 5040 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca 5100 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa 5160 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc 5220 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc 5280 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag 5340 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac 5400 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat 5460 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac 5520 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc 5580 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 5640 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 5700 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 5760 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 5820 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 5880 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 5940 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 6000 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 6060 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 6120 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 6180 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 6240 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 6300 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 6360 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 6420 tctgtggata accgtattac cgccatgcat 6450 //