LOCUS       pDEST-GADT7             9677 bp    DNA     circular      2-DEC-2011
DEFINITION  LMBP 4544.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4544
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 2-DEC-2011
            
            
            
            
COMMENT     VNTNAME|pDEST-GADT7|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pDEST-GADT7" 0 9677 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList 
COMMENT         #0=(CFragment 3 1 0 1128756077 0 "" 0 "" "" "" "" "" "" "pGADT7" "" 0 0 
COMMENT             1 1994 1 1994 1 1990 4 "" "aatt" "" "" "" "" (CObList) 
COMMENT             (CTerm 4 0 0 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "EcoRI" "" 1 0 0 0 1 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 3 1 0 0 0 "" 0 "" "" "" "" "" "" "rfB Gateway cassette" 
COMMENT             "" 0 0 1 1713 1995 3707 1 1713 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 4 0 0 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 4 1 0 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #2=(CFragment 3 1 0 0 0 "" 0 "" "" "" "" "" "" "pGADT7" "" 0 0 1 5970 
COMMENT             3708 9677 2019 7988 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "BamHI" "" 1 0 0 0 1 0 0 0 0 0) 
COMMENT             (CTerm 4 1 0 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BanI" "EcoRI" "NdeI" "PvuII" "XhoI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 6120 6153 1 9677 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #3=(CRSite (CStringList) "BanI" "ggyrcc" 2 0 5 145 0 1550 0 2137 0 6037 
COMMENT            0 7285 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2446 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 1971 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "PvuII" "cagctg" 4 0 3 2346 0 6117 0 6268 0 0 
COMMENT            "") #7=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 3720 0 0 "")) 
COMMENT       (CObList 
COMMENT        #8=(CFSignal (CObList) "GALad" 4 0 0 1492 1899 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1492..1899") 
COMMENT        #9=(CFSignal (CObList) "T7 promoter" 30 0 0 1905 1921 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1905..1921") 
COMMENT        #10=(CFSignal (CObList) "attR1" 0 0 1 1999 2123 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1999..2123)") 
COMMENT        #11=(CFSignal (CObList) "attR2" 0 0 0 3579 3703 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3579..3703") 
COMMENT        #12=(CFSignal (CObList) "chloramphenicol resistance" 4 0 0 2232 2891 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2232..2891") 
COMMENT        #13=(CFSignal (CObList) "ccdB" 4 0 0 3233 3538 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3233..3538") 
COMMENT        #14=(CFSignal (CObList) "pMB1 ori" 33 0 1 6320 6722 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(6320..6722)") 
COMMENT        #15=(CFSignal (CObList) "ampicillin resistance" 4 0 1 7263 8123 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(7263..8123)") 
COMMENT        #16=(CFSignal (CObList) "2mu ori" 33 0 0 8694 9674 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "8694..9674") 
COMMENT        #17=(CFSignal (CObList) "ADH1 terminator" 43 0 0 4105 4206 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4105..4206") 
COMMENT        #18=(CFSignal (CObList) "LEU2" 4 0 1 4409 5503 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4409..5503)") 
COMMENT        #19=(CFSignal (CObList) "HA epitope" 22 0 0 1942 1968 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1942..1968") 
COMMENT        #20=(CFSignal (CObList) "lacUV5 promoter" 30 0 0 2127 2183 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2127..2183") 
COMMENT        #21=(CFSignal (CObList) "lac promoter" 30 0 1 5994 6077 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5994..6077)") 
COMMENT        #22=(CFSignal (CObList) "loxP" 21 0 1 6120 6153 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(6122..6153)") 
COMMENT        #23=(CFSignal (CObList) "loxP" 21 0 1 6231 6264 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(6233..6264)") 
COMMENT        #24=(CFSignal (CObList) "FLP terminator" 43 0 0 8508 8612 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "8508..8612") 
COMMENT        #25=(CFSignal (CObList) "ADH1 promoter" 29 0 0 1077 1481 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1077..1481")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList 
COMMENT        #26=(CFSignal (CObList) "LMBP 4544" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #27=(CFSignal (CObList) "Last modified: 2-DEC-2011" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #28=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #29=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pDEST-GADT7" 2) 5 
COMMENT                   "" 0 4) 
COMMENT              #30=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pDEST-GADT7'" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DMB 4544 (B. Janssens)." 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 12/02/11 03:18" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 9677 bp" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Constructed" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #39=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 1-DEC-2011" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #42=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #43=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #44=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #45=(CLinePar (CParagraph 0 (15 #26# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 4544") 
COMMENT                     #46=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #47=(CLinePar (CParagraph 0 (15 #27# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 2-DEC-2011"))) 
COMMENT                   "Annotations") 
COMMENT              #48=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 10 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #49=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 0 (5 #0# 0) 1 2 1 0 195) 
COMMENT                           (CObjectList 
COMMENT                            #50=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "parent position: from 1 to 1990 (+ fill of 4)" 
COMMENT                                 1) 
COMMENT                            #51=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "original length: 1994" 1) 
COMMENT                            #52=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "molecule position: from 1 to 1994" 1) 
COMMENT                            #53=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) 
COMMENT                                  (CObjectList 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  Start of Molecule" 1))) 
COMMENT                                 "Left Terminus") 
COMMENT                            #55=(CFolderPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #56=(CLinePar 
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COMMENT                                        "  EcoRI site #1" 1) 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "    Completely filled in" 1))) 
COMMENT                                 "Right Terminus"))) 
COMMENT                          "#1: FRAGMENT of <a href=\"vnti:dnarna/pGADT7?sel=1-1990\">pGADT7</a> ") 
COMMENT                     #58=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 0 (5 #1# 0) 1 2 1 0 195) 
COMMENT                           (CObjectList 
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COMMENT                                 "parent position: from 1 to 1713" 1) 
COMMENT                            #60=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "original length: 1713" 1) 
COMMENT                            #61=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "molecule position: from 1995 to 3707" 1) 
COMMENT                            #62=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) 
COMMENT                                  (CObjectList 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  Start of Molecule" 1))) 
COMMENT                                 "Left Terminus") 
COMMENT                            #64=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) 
COMMENT                                  (CObjectList 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  End of Molecule" 1))) 
COMMENT                                 "Right Terminus"))) 
COMMENT                          "#2: FRAGMENT of <a href=\"vnti:dnarna/rfB%20Gateway%20cassette?sel=1-1713\">rfB Gateway cassette</a> ") 
COMMENT                     #66=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 0 (5 #2# 0) 1 2 1 0 195) 
COMMENT                           (CObjectList 
COMMENT                            #67=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "parent position: from 2019 to 7988" 1) 
COMMENT                            #68=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "original length: 5970" 1) 
COMMENT                            #69=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "molecule position: from 3708 to 9677" 1) 
COMMENT                            #70=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) 
COMMENT                                  (CObjectList 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  BamHI site #1" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "    Completely filled in" 1))) 
COMMENT                                 "Left Terminus") 
COMMENT                            #73=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) 
COMMENT                                  (CObjectList 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  End of Molecule" 1))) 
COMMENT                                 "Right Terminus"))) 
COMMENT                          "#3: FRAGMENT of <a href=\"vnti:dnarna/pGADT7?sel=2019-7988\">pGADT7</a> "))) 
COMMENT                   "Component Fragments") 
COMMENT              #75=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #76=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 0 (7 0 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #77=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1999 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1999  End: 2123 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1999..2123)" 1))) "attR1") 
COMMENT                            #81=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3579 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3579  End: 3703" 1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3579..3703" 1))) "attR2"))) 
COMMENT                          "Attenuator (2 total)") 
COMMENT                     #85=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #86=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1492 (3 #8# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1492  End: 1899" 1) 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1492..1899" 1))) "GALad") 
COMMENT                            #90=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2232 (3 #12# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2232  End: 2891" 1) 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2232..2891" 1))) 
COMMENT                                 "chloramphenicol resistance") 
COMMENT                            #94=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3233 (3 #13# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3233  End: 3538" 1) 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3233..3538" 1))) "ccdB") 
COMMENT                            #98=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4409 (3 #18# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4409  End: 5503 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Original Location Description:" 1) 
COMMENT                                   #101=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   complement(4409..5503)" 1))) "LEU2") 
COMMENT                            #102=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 7263 (3 #15# 0) 1 2 2 0 327) 
COMMENT                                   (CObjectList 
COMMENT                                    #103=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 7263  End: 8123 (Complementary)" 
COMMENT                                          1) 
COMMENT                                    #104=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #105=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   complement(7263..8123)" 1))) 
COMMENT                                  "ampicillin resistance"))) "CDS (5 total)") 
COMMENT                     #106=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #107=(CFolderPar 
COMMENT                                   (CGroupPar 
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COMMENT                                    (CObjectList 
COMMENT                                     #108=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 6120  End: 6153 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #109=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #110=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(6122..6153)" 1))) 
COMMENT                                   "loxP") 
COMMENT                             #111=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 6231 (3 #23# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #112=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 6231  End: 6264 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #113=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #114=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(6233..6264)" 1))) 
COMMENT                                   "loxP"))) "Misc. Feature (2 total)") 
COMMENT                     #115=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #116=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 1942 (3 #19# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #117=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 1942  End: 1968" 1) 
COMMENT                                     #118=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #119=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   1942..1968" 1))) "HA epitope"))) 
COMMENT                           "Misc. Marker (1 total)") 
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COMMENT                            (CObjectList 
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COMMENT                                   (CGroupPar 
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COMMENT                                    (CObjectList 
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COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                                     #123=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #124=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   1077..1481" 1))) "ADH1 promoter"))) 
COMMENT                           "Promoter Eukaryotic (1 total)") 
COMMENT                     #125=(CFolderPar 
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COMMENT                            (CObjectList 
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COMMENT                                     #128=(CLinePar 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #129=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   1905..1921" 1))) "T7 promoter") 
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COMMENT                                    (CObjectList 
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COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2127  End: 2183" 1) 
COMMENT                                     #132=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #133=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   2127..2183" 1))) 
COMMENT                                   "lacUV5 promoter") 
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COMMENT                                    (CObjectList 
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COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5994  End: 6077 (Complementary)" 
COMMENT                                           1) 
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FEATURES             Location/Qualifiers
     promoter        1077..1481
                     /vntifkey="29"
                     /label=ADH1\promoter
     CDS             1492..1899
                     /vntifkey="4"
                     /label=GALad
     promoter        1905..1921
                     /vntifkey="30"
                     /label=T7\promoter
     attenuator      complement(1999..2123)
                     /vntifkey="0"
                     /label=attR1
     attenuator      3579..3703
                     /vntifkey="0"
                     /label=attR2
     CDS             2232..2891
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             3233..3538
                     /vntifkey="4"
                     /label=ccdB
     rep_origin      complement(6320..6722)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(7263..8123)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      8694..9674
                     /vntifkey="33"
                     /label=2mu\ori
     terminator      4105..4206
                     /vntifkey="43"
                     /label=ADH1\terminator
     CDS             complement(4409..5503)
                     /vntifkey="4"
                     /label=LEU2
     misc_marker     1942..1968
                     /vntifkey="22"
                     /label=HA\epitope
     promoter        2127..2183
                     /vntifkey="30"
                     /label=lacUV5\promoter
     promoter        complement(5994..6077)
                     /vntifkey="30"
                     /label=lac\promoter
     misc_feature    complement(6120..6153)
                     /vntifkey="21"
                     /label=loxP
     misc_feature    complement(6231..6264)
                     /vntifkey="21"
                     /label=loxP
     terminator      8508..8612
                     /vntifkey="43"
                     /label=FLP\terminator
BASE COUNT     2764 a      2114 c      2090 g      2709 t 
ORIGIN
        1 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc 
       61 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt 
      121 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca 
      181 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc 
      241 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg 
      301 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg 
      361 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa 
      421 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat 
      481 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg 
      541 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt 
      601 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac 
      661 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac 
      721 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt 
      781 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga 
      841 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat 
      901 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc 
      961 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata 
     1021 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca 
     1081 acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat 
     1141 gacaaaaaaa atgatggaag acactaaagg aaaaaattaa cgacaaagac agcaccaaca 
     1201 gatgtcgttg ttccagagct gatgaggggt atctcgaagc acacgaaact ttttccttcc 
     1261 ttcattcacg cacactactc tctaatgagc aacggtatac ggccttcctt ccagttactt 
     1321 gaatttgaaa taaaaaaaag tttgctgtct tgctatcaag tataaataga cctgcaatta 
     1381 ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt ctttttctgc 
     1441 acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa gatggataaa 
     1501 gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg taccgccgcc 
     1561 aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt cactaacagt 
     1621 agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc acaaccaatt 
     1681 gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag taaaattgat 
     1741 gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc gtataacgcg 
     1801 tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata taactatcta 
     1861 ttcgatgatg aagatacccc accaaaccca aaaaaagaga tctttaatac gactcactat 
     1921 agggcgagcg ccgccatgga gtacccatac gacgtaccag attacgctca tatggccatg 
     1981 gaggccagtg aattatcaac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat 
     2041 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa 
     2101 acacaacata tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg 
     2161 cttccggctc gtataatgtg tggattttga gttaggatcc ggcgagattt tcaggagcta 
     2221 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc 
     2281 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg 
     2341 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc 
     2401 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa 
     2461 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg 
     2521 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc 
     2581 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag 
     2641 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg 
     2701 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt 
     2761 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg 
     2821 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg 
     2881 gcggggcgta aagatctgga tccggcttac taaaagccag ataacagtat gcgtatttgc 
     2941 gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa 
     3001 gaggtgtgct atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct 
     3061 caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc 
     3121 cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc 
     3181 cggtttattg aaatgaacgg ctcttttgct gacgagaaca gggactggtg aaatgcagtt 
     3241 taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga 
     3301 tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc 
     3361 agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat 
     3421 gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct 
     3481 cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat 
     3541 gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg 
     3601 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta 
     3661 tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttgatgat ccatcgagct 
     3721 cgagctgcag atgaatcgta gatactgaaa aaccccgcaa gttcacttca actgtgcatc 
     3781 gtgcaccatc tcaatttctt tcatttatac atcgttttgc cttcttttat gtaactatac 
     3841 tcctctaagt ttcaatcttg gccatgtaac ctctgatcta tagaattttt taaatgacta 
     3901 gaattaatgc ccatcttttt tttggaccta aattcttcat gaaaatatat tacgagggct 
     3961 tattcagaag ctttggactt cttcgccaga ggtttggtca agtctccaat caaggttgtc 
     4021 ggcttgtcta ccttgccaga aatttacgaa aagatggaaa agggtcaaat cgttggtaga 
     4081 tacgttgttg acacttctaa ataagcgaat ttcttatgat ttatgatttt tattattaaa 
     4141 taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga 
     4201 aaattcttat tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagcat 
     4261 gaggtcgctc ttattgacca cacctctacc ggccggtcga aattccccta ccctatgaac 
     4321 atattccatt ttgtaatttc gtgtcgtttc tattatgaat ttcatttata aagtttatgt 
     4381 acaaatatca taaaaaaaga gaatcttttt aagcaaggat tttcttaact tcttcggcga 
     4441 cagcatcacc gacttcggtg gtactgttgg aaccacctaa atcaccagtt ctgatacctg 
     4501 catccaaaac ctttttaact gcatcttcaa tggccttacc ttcttcaggc aagttcaatg 
     4561 acaatttcaa catcattgca gcagacaaga tagtggcgat agggttgacc ttattctttg 
     4621 gcaaatctgg agcagaaccg tggcatggtt cgtacaaacc aaatgcggtg ttcttgtctg 
     4681 gcaaagaggc caaggacgca gatggcaaca aacccaagga acctgggata acggaggctt 
     4741 catcggagat gatatcacca aacatgttgc tggtgattat aataccattt aggtgggttg 
     4801 ggttcttaac taggatcatg gcggcagaat caatcaattg atgttgaacc ttcaatgtag 
     4861 gaaattcgtt cttgatggtt tcctccacag tttttctcca taatcttgaa gaggccaaaa 
     4921 cattagcttt atccaaggac caaataggca atggtggctc atgttgtagg gccatgaaag 
     4981 cggccattct tgtgattctt tgcacttctg gaacggtgta ttgttcacta tcccaagcga 
     5041 caccatcacc atcgtcttcc tttctcttac caaagtaaat acctcccact aattctctga 
     5101 caacaacgaa gtcagtacct ttagcaaatt gtggcttgat tggagataag tctaaaagag 
     5161 agtcggatgc aaagttacat ggtcttaagt tggcgtacaa ttgaagttct ttacggattt 
     5221 ttagtaaacc ttgttcaggt ctaacactac ctgtacccca tttaggacca cccacagcac 
     5281 ctaacaaaac ggcatcaacc ttcttggagg cttccagcgc ctcatctgga agtgggacac 
     5341 ctgtagcgtc gatagcagca ccaccaatta aatgattttc gaaatcgaac ttgacattgg 
     5401 aacgaacatc agaaatagct ttaagaacct taatggcttc ggctgtgatt tcttgaccaa 
     5461 cgtggtcacc tggcaaaacg acgatcttct taggggcaga cattagaatg gtatatcctt 
     5521 gaaatatata tatatattgc tgaaatgtaa aaggtaagaa aagttagaaa gtaagacgat 
     5581 tgctaaccac ctattggaaa aaacaatagg tccttaaata atattgtcaa cttcaagtat 
     5641 tgtgatgcaa gcatttagtc atgaacgctt ctctattcta tatgaaaagc cggttccggc 
     5701 gctctcacct ttcctttttc tcccaatttt tcagttgaaa aaggtatatg cgtcaggcga 
     5761 cctctgaaat taacaaaaaa tttccagtca tcgaatttga ttctgtgcga tagcgcccct 
     5821 gtgtgttctc gttatgttga ggaaaaaaat aatggttgct aagagattcg aactcttgca 
     5881 tcttacgata cctgagtatt cccacagttg gggatctcga ctctagctag aggatcaatt 
     5941 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 
     6001 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 
     6061 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctga 
     6121 taacttcgta taatgtatgc tatacgaagt tattaggtct gaagaggagt ttacgtccag 
     6181 ccaagctagc ttggctgcag gtcgagcggc cgcgatccgg aacccttaat ataacttcgt 
     6241 ataatgtatg ctatacgaag ttatcagctg cattaatgaa tcggccaacg cgcggggaga 
     6301 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 
     6361 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 
     6421 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 
     6481 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 
     6541 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 
     6601 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 
     6661 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 
     6721 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 
     6781 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 
     6841 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 
     6901 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 
     6961 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 
     7021 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 
     7081 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 
     7141 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 
     7201 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 
     7261 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 
     7321 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 
     7381 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 
     7441 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 
     7501 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 
     7561 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 
     7621 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 
     7681 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 
     7741 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 
     7801 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 
     7861 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 
     7921 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 
     7981 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 
     8041 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 
     8101 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 
     8161 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
     8221 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 
     8281 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat 
     8341 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg 
     8401 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc 
     8461 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccataa cgcatttaag 
     8521 cataaacacg cactatgccg ttcttctcat gtatatatat atacaggcaa cacgcagata 
     8581 taggtgcgac gtgaacagtg agctgtatgt gcgcagctcg cgttgcattt tcggaagcgc 
     8641 tcgttttcgg aaacgctttg aagttcctat tccgaagttc ctattctcta gctagaaagt 
     8701 ataggaactt cagagcgctt ttgaaaacca aaagcgctct gaagacgcac tttcaaaaaa 
     8761 ccaaaaacgc accggactgt aacgagctac taaaatattg cgaataccgc ttccacaaac 
     8821 attgctcaaa agtatctctt tgctatatat ctctgtgcta tatccctata taacctaccc 
     8881 atccaccttt cgctccttga acttgcatct aaactcgacc tctacatttt ttatgtttat 
     8941 ctctagtatt actctttaga caaaaaaatt gtagtaagaa ctattcatag agtgaatcga 
     9001 aaacaatacg aaaatgtaaa catttcctat acgtagtata tagagacaaa atagaagaaa 
     9061 ccgttcataa ttttctgacc aatgaagaat catcaacgct atcactttct gttcacaaag 
     9121 tatgcgcaat ccacatcggt atagaatata atcggggatg cctttatctt gaaaaaatgc 
     9181 acccgcagct tcgctagtaa tcagtaaacg cgggaagtgg agtcaggctt tttttatgga 
     9241 agagaaaata gacaccaaag tagccttctt ctaaccttaa cggacctaca gtgcaaaaag 
     9301 ttatcaagag actgcattat agagcgcaca aaggagaaaa aaagtaatct aagatgcttt 
     9361 gttagaaaaa tagcgctctc gggatgcatt tttgtagaac aaaaaagaag tatagattct 
     9421 ttgttggtaa aatagcgctc tcgcgttgca tttctgttct gtaaaaatgc agctcagatt 
     9481 ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt tttacaaaaa tgaagcacag 
     9541 attcttcgtt ggtaaaatag cgctttcgcg ttgcatttct gttctgtaaa aatgcagctc 
     9601 agattctttg tttgaaaaat tagcgctctc gcgttgcatt tttgttctac aaaatgaagc 
     9661 acagatgctt cgttgct 
//
