ID pDEST-GADT7 standard; circular DNA; ; 9677 BP. DE LMBP 4544. CC Accession number: LMBP 4544 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 2-DEC-2011 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 1077..1481 FT /vntifkey="29" FT /label=ADH1\promoter FT CDS 1492..1899 FT /vntifkey="4" FT /label=GALad FT promoter 1905..1921 FT /vntifkey="30" FT /label=T7\promoter FT attenuator complement(1999..2123) FT /vntifkey="0" FT /label=attR1 FT attenuator 3579..3703 FT /vntifkey="0" FT /label=attR2 FT CDS 2232..2891 FT /vntifkey="4" FT /label=chloramphenicol\resistance FT CDS 3233..3538 FT /vntifkey="4" FT /label=ccdB FT rep_origin complement(6320..6722) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(7263..8123) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin 8694..9674 FT /vntifkey="33" FT /label=2mu\ori FT terminator 4105..4206 FT /vntifkey="43" FT /label=ADH1\terminator FT CDS complement(4409..5503) FT /vntifkey="4" FT /label=LEU2 FT misc_marker 1942..1968 FT /vntifkey="22" FT /label=HA\epitope FT promoter 2127..2183 FT /vntifkey="30" FT /label=lacUV5\promoter FT promoter complement(5994..6077) FT /vntifkey="30" FT /label=lac\promoter FT misc_feature complement(6120..6153) FT /vntifkey="21" FT /label=loxP FT misc_feature complement(6231..6264) FT /vntifkey="21" FT /label=loxP FT terminator 8508..8612 FT /vntifkey="43" FT /label=FLP\terminator SQ Sequence 9677 BP; 2764 A; 2114 C; 2090 G; 2709 t; tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc 60 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt 120 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca 180 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc 240 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg 300 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg 360 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa 420 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat 480 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg 540 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt 600 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac 660 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac 720 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt 780 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga 840 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat 900 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc 960 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata 1020 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca 1080 acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat 1140 gacaaaaaaa atgatggaag acactaaagg aaaaaattaa cgacaaagac agcaccaaca 1200 gatgtcgttg ttccagagct gatgaggggt atctcgaagc acacgaaact ttttccttcc 1260 ttcattcacg cacactactc tctaatgagc aacggtatac ggccttcctt ccagttactt 1320 gaatttgaaa taaaaaaaag tttgctgtct tgctatcaag tataaataga cctgcaatta 1380 ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt ctttttctgc 1440 acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa gatggataaa 1500 gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg taccgccgcc 1560 aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt cactaacagt 1620 agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc acaaccaatt 1680 gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag taaaattgat 1740 gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc gtataacgcg 1800 tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata taactatcta 1860 ttcgatgatg aagatacccc accaaaccca aaaaaagaga tctttaatac gactcactat 1920 agggcgagcg ccgccatgga gtacccatac gacgtaccag attacgctca tatggccatg 1980 gaggccagtg aattatcaac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat 2040 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa 2100 acacaacata tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg 2160 cttccggctc gtataatgtg tggattttga gttaggatcc ggcgagattt tcaggagcta 2220 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc 2280 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg 2340 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc 2400 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa 2460 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg 2520 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc 2580 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag 2640 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg 2700 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt 2760 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg 2820 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg 2880 gcggggcgta aagatctgga tccggcttac taaaagccag ataacagtat gcgtatttgc 2940 gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa 3000 gaggtgtgct atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct 3060 caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc 3120 cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc 3180 cggtttattg aaatgaacgg ctcttttgct gacgagaaca gggactggtg aaatgcagtt 3240 taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga 3300 tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc 3360 agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat 3420 gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct 3480 cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat 3540 gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg 3600 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta 3660 tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttgatgat ccatcgagct 3720 cgagctgcag atgaatcgta gatactgaaa aaccccgcaa gttcacttca actgtgcatc 3780 gtgcaccatc tcaatttctt tcatttatac atcgttttgc cttcttttat gtaactatac 3840 tcctctaagt ttcaatcttg gccatgtaac ctctgatcta tagaattttt taaatgacta 3900 gaattaatgc ccatcttttt tttggaccta aattcttcat gaaaatatat tacgagggct 3960 tattcagaag ctttggactt cttcgccaga ggtttggtca agtctccaat caaggttgtc 4020 ggcttgtcta ccttgccaga aatttacgaa aagatggaaa agggtcaaat cgttggtaga 4080 tacgttgttg acacttctaa ataagcgaat ttcttatgat ttatgatttt tattattaaa 4140 taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga 4200 aaattcttat tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagcat 4260 gaggtcgctc ttattgacca cacctctacc ggccggtcga aattccccta ccctatgaac 4320 atattccatt ttgtaatttc gtgtcgtttc tattatgaat ttcatttata aagtttatgt 4380 acaaatatca taaaaaaaga gaatcttttt aagcaaggat tttcttaact tcttcggcga 4440 cagcatcacc gacttcggtg gtactgttgg aaccacctaa atcaccagtt ctgatacctg 4500 catccaaaac ctttttaact gcatcttcaa tggccttacc ttcttcaggc aagttcaatg 4560 acaatttcaa catcattgca gcagacaaga tagtggcgat agggttgacc ttattctttg 4620 gcaaatctgg agcagaaccg tggcatggtt cgtacaaacc aaatgcggtg ttcttgtctg 4680 gcaaagaggc caaggacgca gatggcaaca aacccaagga acctgggata acggaggctt 4740 catcggagat gatatcacca aacatgttgc tggtgattat aataccattt aggtgggttg 4800 ggttcttaac taggatcatg gcggcagaat caatcaattg atgttgaacc ttcaatgtag 4860 gaaattcgtt cttgatggtt tcctccacag tttttctcca taatcttgaa gaggccaaaa 4920 cattagcttt atccaaggac caaataggca atggtggctc atgttgtagg gccatgaaag 4980 cggccattct tgtgattctt tgcacttctg gaacggtgta ttgttcacta tcccaagcga 5040 caccatcacc atcgtcttcc tttctcttac caaagtaaat acctcccact aattctctga 5100 caacaacgaa gtcagtacct ttagcaaatt gtggcttgat tggagataag tctaaaagag 5160 agtcggatgc aaagttacat ggtcttaagt tggcgtacaa ttgaagttct ttacggattt 5220 ttagtaaacc ttgttcaggt ctaacactac ctgtacccca tttaggacca cccacagcac 5280 ctaacaaaac ggcatcaacc ttcttggagg cttccagcgc ctcatctgga agtgggacac 5340 ctgtagcgtc gatagcagca ccaccaatta aatgattttc gaaatcgaac ttgacattgg 5400 aacgaacatc agaaatagct ttaagaacct taatggcttc ggctgtgatt tcttgaccaa 5460 cgtggtcacc tggcaaaacg acgatcttct taggggcaga cattagaatg gtatatcctt 5520 gaaatatata tatatattgc tgaaatgtaa aaggtaagaa aagttagaaa gtaagacgat 5580 tgctaaccac ctattggaaa aaacaatagg tccttaaata atattgtcaa cttcaagtat 5640 tgtgatgcaa gcatttagtc atgaacgctt ctctattcta tatgaaaagc cggttccggc 5700 gctctcacct ttcctttttc tcccaatttt tcagttgaaa aaggtatatg cgtcaggcga 5760 cctctgaaat taacaaaaaa tttccagtca tcgaatttga ttctgtgcga tagcgcccct 5820 gtgtgttctc gttatgttga ggaaaaaaat aatggttgct aagagattcg aactcttgca 5880 tcttacgata cctgagtatt cccacagttg gggatctcga ctctagctag aggatcaatt 5940 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 6000 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 6060 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctga 6120 taacttcgta taatgtatgc tatacgaagt tattaggtct gaagaggagt ttacgtccag 6180 ccaagctagc ttggctgcag gtcgagcggc cgcgatccgg aacccttaat ataacttcgt 6240 ataatgtatg ctatacgaag ttatcagctg cattaatgaa tcggccaacg cgcggggaga 6300 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 6360 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 6420 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 6480 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 6540 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 6600 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 6660 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 6720 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 6780 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 6840 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 6900 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 6960 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 7020 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 7080 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 7140 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 7200 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 7260 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 7320 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 7380 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 7440 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 7500 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 7560 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 7620 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 7680 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 7740 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 7800 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 7860 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 7920 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 7980 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 8040 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 8100 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 8160 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 8220 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 8280 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat 8340 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg 8400 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc 8460 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccataa cgcatttaag 8520 cataaacacg cactatgccg ttcttctcat gtatatatat atacaggcaa cacgcagata 8580 taggtgcgac gtgaacagtg agctgtatgt gcgcagctcg cgttgcattt tcggaagcgc 8640 tcgttttcgg aaacgctttg aagttcctat tccgaagttc ctattctcta gctagaaagt 8700 ataggaactt cagagcgctt ttgaaaacca aaagcgctct gaagacgcac tttcaaaaaa 8760 ccaaaaacgc accggactgt aacgagctac taaaatattg cgaataccgc ttccacaaac 8820 attgctcaaa agtatctctt tgctatatat ctctgtgcta tatccctata taacctaccc 8880 atccaccttt cgctccttga acttgcatct aaactcgacc tctacatttt ttatgtttat 8940 ctctagtatt actctttaga caaaaaaatt gtagtaagaa ctattcatag agtgaatcga 9000 aaacaatacg aaaatgtaaa catttcctat acgtagtata tagagacaaa atagaagaaa 9060 ccgttcataa ttttctgacc aatgaagaat catcaacgct atcactttct gttcacaaag 9120 tatgcgcaat ccacatcggt atagaatata atcggggatg cctttatctt gaaaaaatgc 9180 acccgcagct tcgctagtaa tcagtaaacg cgggaagtgg agtcaggctt tttttatgga 9240 agagaaaata gacaccaaag tagccttctt ctaaccttaa cggacctaca gtgcaaaaag 9300 ttatcaagag actgcattat agagcgcaca aaggagaaaa aaagtaatct aagatgcttt 9360 gttagaaaaa tagcgctctc gggatgcatt tttgtagaac aaaaaagaag tatagattct 9420 ttgttggtaa aatagcgctc tcgcgttgca tttctgttct gtaaaaatgc agctcagatt 9480 ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt tttacaaaaa tgaagcacag 9540 attcttcgtt ggtaaaatag cgctttcgcg ttgcatttct gttctgtaaa aatgcagctc 9600 agattctttg tttgaaaaat tagcgctctc gcgttgcatt tttgttctac aaaatgaagc 9660 acagatgctt cgttgct 9677 //