ID pdEYFP standard; circular DNA; UNA; 5935 BP. DE LMBP 4547. CC Accession number: LMBP 4547 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 15-JUN-2012 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 1..568 FT /vntifkey="29" FT /label=hCMV-IE\promoter\and\enhancer FT /note="Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer" FT attenuator complement(675..799) FT /vntifkey="0" FT /label=attR1 FT /note="bacteriophage lambda attR1 recombination site" FT Promoter 807..859 FT /vntifkey="21" FT /label=lacUV5\promoter FT /note="Unknown feature type:Promoter" FT CDS 908..1567 FT /vntifkey="4" FT /label=chloramphenicol\resistance FT CDS 1909..2214 FT /vntifkey="4" FT /label=ccdB FT /note="B gene of the control of cell death locus of the E. coli F plasmid (ccdB, lethal gene)" FT attenuator 2255..2379 FT /vntifkey="0" FT /label=attR2 FT /note="bacteriophage lambda attR2 recombination site" FT CDS 2396..3115 FT /vntifkey="4" FT /label=EYFP FT /note="A. victoria green-fluorescent protein DNA; enhanced yellow-green fluorescent variant" FT terminator 3216..3312 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(3216..3312) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT rep_origin complement(3366..3821) FT /vntifkey="33" FT /label=f1\ori FT /note="Phage f1 origin" FT promoter 3822..3917 FT /vntifkey="30" FT /label=amp\promoter FT RBS 3918..3952 FT /vntifkey="32" FT /label=5'\UTR\of\amp,\incl.\RBS FT promoter 3973..4254 FT /vntifkey="29" FT /label=SV40\early\promoter FT /note="Simian virus 40 early promoter (SV40 early)" FT rep_origin 4211..4286 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT promoter 4319..4371 FT /vntifkey="30" FT /label=lacUV5\promoter FT CDS 4409..4942 FT /vntifkey="4" FT /label=gentamycin\resistance FT rep_origin 5603..5929 FT /vntifkey="33" FT /label=pMB1\ori SQ Sequence 5935 BP; 1536 A; 1490 C; 1460 G; 1447 t; 2 other; tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 60 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 120 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 180 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 240 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 300 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 360 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 420 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 480 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 540 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 600 ccggactcag atctcgagct caagcttcka attctgcagt cracggtacc gcgggcccgg 660 gatccaccgg atcaacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa 720 atatcaatat attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac 780 aacatatcca gtcactatgg cggccgcatt aggcacccca ggctttacac tttatgcttc 840 cggctcgtat aatgtgtgga ttttgagtta ggatccggcg agattttcag gagctaagga 900 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg 960 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca 1020 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc 1080 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa 1140 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca 1200 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca 1260 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt 1320 tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt 1380 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac 1440 gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tctgtgatgg 1500 cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg 1560 ggcgtaaaga tctggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc 1620 tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg 1680 tgtgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag 1740 gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc 1800 gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt 1860 ttattgaaat gaacggctct tttgctgacg agaacaggga ctggtgaaat gcagtttaag 1920 gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt 1980 attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat 2040 aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg 2100 accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc 2160 caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca 2220 ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat gttgtgtttt 2280 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc 2340 attttacgtt tctcgttcag ctttcttgta caaagtggtt gatccggtcg ccaccatggt 2400 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 2460 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 2520 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 2580 gaccaccttc ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca 2640 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 2700 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 2760 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 2820 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 2880 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 2940 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 3000 gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 3060 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg 3120 ccgcgactct agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa 3180 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac 3240 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 3300 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa 3360 ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 3420 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 3480 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 3540 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 3600 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 3660 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 3720 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 3780 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg 3840 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 3900 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc 3960 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 4020 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 4080 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 4140 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 4200 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 4260 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgcgca 4320 ttaggcaccc caggctttac actttatgct tcctgctcgt ataatgtgtg gattttgagt 4380 taggatccgg cgaagctaag gagctaaaat gttacgcagc agcaacgatg ttacgcagca 4440 gggcagtcgc cctaaaacaa agttaggtgg ctcaagtatg ggcatcattc gcacatgtag 4500 gctcggccct gaccaagtca aatccatgcg ggctgctctt gatcttttcg gtcgtgagtt 4560 cggagacgta gccacctact cccaacatca gccggactcc gattacctcg ggaacttgct 4620 ccgtagtaag acattcatcg cgcttgctgc cttcgaccaa gaagcggttg ttggcgctct 4680 cgcggcttac gttctgccca agtttgagca gccgcgtagt gagatctata tctatgatct 4740 cgcagtctcc ggcgagcacc ggaggcaggg cattgccacc gcgctcatca atctcctcaa 4800 gcatgaggcc aacgcgcttg gtgcttatgt gatctacgtg caagcagatt acggtgacga 4860 tcccgcagtg gctctctata caaagttggg catacgggaa gaagtgatgc actttgatat 4920 cgacccaagt accgccacct aaacgcgtcc accgagaccc cattggggcc aatacgcccg 4980 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc 5040 caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga 5100 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 5160 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat 5220 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 5280 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 5340 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 5400 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 5460 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 5520 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 5580 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 5640 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 5700 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 5760 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 5820 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat 5880 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcat 5935 //