LOCUS       pCAGGS-mCASP-12-C298A 7171 bp dsDNACircular
DEFINITION  LMBP 4573.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     CDS             complement(4448..5308)
                     /label=AMP
     promoter        5827..7168
                     /label=AG\promoter
     promoter        5444..5821
                     /label=hCMV\enhancer
     terminator      2461..2552
                     /label=ß-globin\polyA
     promoter        complement(2987..3069)
                     /label=lac\promoter
     rep_origin      complement(3506..3907)
                     /label=pMB1\ori
     terminator      3316..3391
                     /label=SV40\polyA\early
     terminator      complement(3316..3391)
                     /label=SV40\polyA\late
     rep_origin      3211..3286
                     /label=SV40\ori
     CDS             118..1377
                     /note="mCASP-12 catalytic inactive mutant (at amino acid position 298: Cys -> Ala)."
                     /label=mCASP-12\mutant
     intron          6196..7120
                     /label=intron\AG\promoter
ORIGIN
        1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg 
       61 ggatccccgg gaattcggca cgagggcacg agcggcacga gcggcacgag cacagccatg 
      121 gcggccagga ggacacatga aagagatcca atctacaaga tcaaaggttt ggccaaggac 
      181 atgctggatg gggtttttga tgacctggtg gagaagaatg ttttaaatgg agatgagtta 
      241 ctcaaaatag gggaaagtgc gagtttcatc ctgaacaagg ctgagaatct ggttgagaac 
      301 ttcttagaga aaacagacat ggcaggaaaa atatttgctg gccacattgc caattcccag 
      361 gaacagctga gtttacaatt ttctaatgat gaggatgatg gacctcagaa gatatgtaca 
      421 ccttcttctc catcagaatc caagagaaaa gtagaggatg atgaaatgga ggtaaatgct 
      481 ggattggccc atgaatcaca tctaatgctg acagctcctc atggactcca gagctcagaa 
      541 gtccaagata cactgaagct ttgtccacgt gatcagtttt gtaagataaa gacagaaagg 
      601 gcaaaagaga tatatccagt gatggagaag gagggacgaa cacgtctggc tctcatcatc 
      661 tgcaacaaaa agtttgacta cctttttgat agagataatg ctgatactga cattttgaac 
      721 atgcaagaac tacttgaaaa tcttggatac tctgtggtgt taaaggaaaa ccttacagct 
      781 caggaaatgg agacagagtt aatgcagttt gctggccgtc cagagcacca gtcctcagac 
      841 agcacattcc tggtgtttat gtcccatggc atcctggaag gaatctgtgg ggtgaagcac 
      901 cgaaacaaaa agccagatgt tcttcatgat gacactatct tcaaaatttt caacaactct 
      961 aactgtcgga gtctgagaaa caaacccaag attctcatca tgcaggccgc cagaggcaga 
     1021 tataatggaa ctatttgggt atccacaaac aaagggatag ccactgctga tacagatgag 
     1081 gaacgtgtgt tgagctgtaa atggaataat agtataacaa aggcccatgt ggagacagat 
     1141 ttcattgctt tcaaatcttc taccccacat aatatttctt ggaaggtagg caagactggt 
     1201 tccctcttca tttccaaact cattgactgc ttcaaaaagt actgttggtg ttatcatttg 
     1261 gaggaaattt ttcgaaaggt tcaacactca tttgaggtcc caggtgaact gacccagatg 
     1321 cccactattg agagagtatc catgacacgc tatttctacc tttttcccgg gaattagcac 
     1381 aggcaactct catgcagttc ccagtcaagt attgctgtag ctgagaagaa aagaaaattc 
     1441 caagatccca ggatttttaa atgtgtaaaa cttttcagtt agatatttta tacaatatca 
     1501 taaggctaga taatttccat aggttatttt taggggaaac ctgatacgta aaattttgca 
     1561 tgaaaagtat agattcgttc cttccttcca aatctgctca tcttttactt tattttatta 
     1621 aatttatatt tgcacatttt tgtaaaatga ataaaggaaa aagacactaa agaacatgtc 
     1681 tgaacatatc ctggaacttc ttctttgtaa aatgaataaa ggaaaaagac actaaagaac 
     1741 aaagatgata caccttttga tgaaccactt tcacctataa attagtagta agaatttact 
     1801 cacaaggaag atcttatcta caatgtagct gagaaatgaa actgttgcat atgtaggtaa 
     1861 aactaatcat cttttaggaa taagataaag aggtttatgt ccactgtttg tctctggcat 
     1921 acatggtttt catgcctagt ggctacctac cattcctttc aatcaatttc ttaggatata 
     1981 attgacaaat ttatgctatg tttataattt ttctcttaaa tgcctttgta ataagatata 
     2041 ctcacacata cacatttaag attctgtcta ttagtgttat tgaaataaat cctagaaaca 
     2101 aaaattaaaa gtttaaagca ttaaagacaa ttttgagaca aaatattcta tgaaattcta 
     2161 tgcaaaattg aattcttcta caagtatgtg atgaagccca cctaccacta tttttgtcat 
     2221 cttgaggatg ttatatcatt atttttactt aactgagctc aaattaaaat ctttgtttat 
     2281 tctttgttct ataaaaaaaa aaaaaaaaaa actcgtgccg aattcgatct ctttttttgg 
     2341 gtttggtggg gtatcttcat catcgaatag atagttatat acatcatcca ttgtagtggt 
     2401 attaaacatc cctgtagtga ttccaaacgc gccaatgccc tggctcacaa ataccactga 
     2461 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact 
     2521 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc 
     2581 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt 
     2641 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga 
     2701 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg 
     2761 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta 
     2821 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc 
     2881 atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgtaatc 
     2941 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 
     3001 agccggaagc ataaagtgta aagcctgggt gcctaatgag tgagctaact cacattaatt 
     3061 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc 
     3121 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 
     3181 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 
     3241 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 
     3301 gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat aaagcaatag 
     3361 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 
     3421 actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg 
     3481 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 
     3541 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 
     3601 acagaatcag ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 
     3661 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 
     3721 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 
     3781 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 
     3841 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 
     3901 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 
     3961 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 
     4021 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 
     4081 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 
     4141 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 
     4201 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 
     4261 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 
     4321 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 
     4381 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 
     4441 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 
     4501 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 
     4561 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 
     4621 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 
     4681 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 
     4741 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 
     4801 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 
     4861 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 
     4921 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 
     4981 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 
     5041 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 
     5101 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 
     5161 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 
     5221 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 
     5281 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 
     5341 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 
     5401 taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgacattg attattgact 
     5461 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 
     5521 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 
     5581 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 
     5641 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 
     5701 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 
     5761 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 
     5821 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc 
     5881 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg 
     5941 ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaaa 
     6001 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg 
     6061 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgt tgcgcgctgc 
     6121 cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc 
     6181 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc 
     6241 ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg 
     6301 gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg 
     6361 agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg 
     6421 ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc 
     6481 ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca 
     6541 gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct 
     6601 gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg 
     6661 ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg 
     6721 gagggctcgg gggaaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc 
     6781 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc 
     6841 ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg 
     6901 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc 
     6961 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct 
     7021 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc 
     7081 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt 
     7141 tattgtgctg tctcatcatt ttggcaaaga a 
//
