LOCUS       p415GALLmBAX 7237 bp dsDNA    Circular
DEFINITION  LMBP 4575.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     promoter        3100..3116
                     /label=T7\promoter
     CDS             complement(663..1769)
                     /label=LEU2
     terminator      complement(3137..3384)
                     /label=CYC1\terminator
     rep_origin      complement(2474..2929)
                     /label=f1\ori
     CDS             complement(3427..4005)
                     /label=mBAX
     rep_origin      6665..7183
                     /label=CEN6/ARSH4
     CDS             complement(5663..6523)
                     /label=AMP
     promoter        complement(4465..4481)
                     /label=T3\promoter
     promoter        complement(4545..4628)
                     /label=lac\promoter
     rep_origin      4720..5122
                     /label=pMB1\ori
     promoter        complement(4076..4449)
                     /label=GALL\promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc 
      241 accattatgg gaaatggttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca 
      301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat 
      361 taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc 
      421 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc 
      481 aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt 
      541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg 
      601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct 
      661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg 
      721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct 
      781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac 
      841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat 
      901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc 
      961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg 
     1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca 
     1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc 
     1141 acagtttttc tccataatct tgaagaggcc aaaacattag ctttatccaa ggaccaaata 
     1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact 
     1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc 
     1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca 
     1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt 
     1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca 
     1501 ctaccggtac cccatttagg accacccaca gcacctaaca aaacggcatc aaccttcttg 
     1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca 
     1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga 
     1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc 
     1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata 
     1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat 
     1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat 
     1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct 
     1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca 
     2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat 
     2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga 
     2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg 
     2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt 
     2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg 
     2341 atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt ggatatacta 
     2401 gaagttctcc tcgaccgtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa 
     2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt 
     2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 
     2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 
     2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 
     2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 
     2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 
     2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 
     2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg 
     2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 
     3001 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 
     3061 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc 
     3121 gaattgggta ccggccgcaa attaaagcct tcgagcgtcc caaaaccttc tcaagcaagg 
     3181 ttttcagtat aatgttacat gcgtacacgc gtctgtacag aaaaaaaaga aaaatttgaa 
     3241 atataaataa cgttcttaat actaacataa ctataaaaaa ataaataggg acctagactt 
     3301 caggttgtct aactccttcc ttttcggtta gagcggatgt ggggggaggg cgtgaatgta 
     3361 agcgtgacat aactaattac atgactcgag gtcgacggta tcgataagct tgcatgcctg 
     3421 caggtctcag cccatcttct tccagatggt gagcgaggcg gtgaggactc cagccacaaa 
     3481 gatggtcact gtctgccatg tgggggtccc gaagtaggag aggaggcctt cccagccacc 
     3541 ctggtcttgg atccagacaa gcagccgctc acggaggaag tccagtgtcc agcccatgat 
     3601 ggttctgatc agctcgggca ctttagtgca cagggccttg agcaccagtt tgctagcaaa 
     3661 gtagaagagg gcaaccacgc ggccccagtt gaagttgcca tcagcaaaca tgtcagctgc 
     3721 cacccggaag aagacctctc ggggggagtc cgtgtccacg tcagcaatca tcctctgcag 
     3781 ctccatattg ctatccagtt catctccaat tcgccggaga cactcgctca gcttcttggt 
     3841 ggacgcatcc tggggcggct gctccaaggt cagctcaggt gtctccccag ccatcctccc 
     3901 tgctcgatcc tggatgaaac cctgtagcaa aaaggcccct gtcttcatga tctgttcaga 
     3961 gctggtgggc ccgccgctcc caagctgctc cccggacccg tccatgactc tagatatagt 
     4021 tttttctcct tgacgttaaa gtatagaggt atattaacaa ttttttgttg atacttttat 
     4081 tacatttgaa taagaagtaa tacaaaccga aaatgttgaa agtattagtt aaagtggtta 
     4141 tgcagttttt gcatttatat atctgttaat agatcaaaaa tcatcgcttc gctgattaat 
     4201 taccccagaa ataaggctaa aaaactaatc gcattatcat cctatggttg ttaatttgat 
     4261 tcgttcattt gaaggtttgt ggggccaggt tactgccaat ttttcctctt cataaccata 
     4321 aaagctagta ttgtagaatc tttattgttc ggagcagtgc ggcgcgaggc acatctgcgt 
     4381 ttcaggaacg cgaccggtga agacgaggac gcacggagga gagtcttcct tcggagggct 
     4441 gtcagagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat 
     4501 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacataggag 
     4561 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gaggtaactc acattaattg 
     4621 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 
     4681 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 
     4741 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 
     4801 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 
     4861 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 
     4921 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 
     4981 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 
     5041 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 
     5101 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 
     5161 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 
     5221 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 
     5281 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 
     5341 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 
     5401 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 
     5461 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 
     5521 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 
     5581 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 
     5641 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 
     5701 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 
     5761 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 
     5821 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 
     5881 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 
     5941 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 
     6001 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 
     6061 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 
     6121 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 
     6181 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 
     6241 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 
     6301 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 
     6361 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 
     6421 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 
     6481 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 
     6541 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 
     6601 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcct 
     6661 tttcatcacg tgctataaaa ataattataa tttaaatttt ttaatataaa tatataaatt 
     6721 aaaaatagaa agtaaaaaaa gaaattaaag aaaaaatagt ttttgttttc cgaagatgta 
     6781 aaagactcta gggggatcgc caacaaatac taccttttat cttgctcttc ctgctctcag 
     6841 gtattaatgc cgaattgttt catcttgtct gtgtagaaga ccacacacga aaatcctgtg 
     6901 attttacatt ttacttatcg ttaatcgaat gtatatctat ttaatctgct tttcttgtct 
     6961 aataaatata tatgtaaagt acgctttttg ttgaaatttt ttaaaccttt gtttattttt 
     7021 ttttcttcat tccgtaactc ttctaccttc tttatttact ttctaaaatc caaatacaaa 
     7081 acataaaaat aaataaacac agagtaaatt cccaaattat tccatcatta aaagatacga 
     7141 ggcgcgtgta agttacaggc aagcgatccg tcctaagaaa ccattattat catgacatta 
     7201 acctataaaa ataggcgtat cacgaggccc tttcgtc 
//
