LOCUS       p425GALLmBAX 8071 bp dsDNA    Circular
DEFINITION  LMBP 4576.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     promoter        3100..3116
                     /label=T7\promoter
     CDS             complement(663..1769)
                     /label=LEU2
     terminator      complement(3137..3384)
                     /label=CYC1\terminator
     rep_origin      2474..2929
                     /label=f1\ori
     CDS             complement(3427..4005)
                     /label=mBAX
     CDS             complement(5669..6529)
                     /label=AMP
     promoter        complement(4082..4455)
                     /label=GALL\promoter
     promoter        complement(4471..4487)
                     /label=T3\promoter
     promoter        complement(4551..4634)
                     /label=lac\promoter
     rep_origin      complement(6661..7641)
                     /label=2mu\ori
     rep_origin      complement(4726..5128)
                     /label=pMB1\ori
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc 
      241 accattatgg gaaatgcttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca 
      301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat 
      361 taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc 
      421 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc 
      481 aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt 
      541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg 
      601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct 
      661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg 
      721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct 
      781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac 
      841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat 
      901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc 
      961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg 
     1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca 
     1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc 
     1141 acagtttttc tccataatct tgaagaggcc aaaagattag ctttatccaa ggaccaaata 
     1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact 
     1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc 
     1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca 
     1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt 
     1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca 
     1501 ctaccggtac cccatttagg accagccaca gcacctaaca aaacggcatc aaccttcttg 
     1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca 
     1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga 
     1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc 
     1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata 
     1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat 
     1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat 
     1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct 
     1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca 
     2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat 
     2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga 
     2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg 
     2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt 
     2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg 
     2341 atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt ggatatacta 
     2401 gaagttctcc tcgaccgtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa 
     2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt 
     2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 
     2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 
     2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 
     2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 
     2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 
     2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 
     2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg 
     2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 
     3001 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 
     3061 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc 
     3121 gaattgggta ccggccgcaa attaaagcct tcgagcgtcc caaaaccttc tcaagcaagg 
     3181 ttttcagtat aatgttacat gcgtacacgc gtctgtacag aaaaaaaaga aaaatttgaa 
     3241 atataaataa cgttcttaat actaacataa ctataaaaaa ataaataggg acctagactt 
     3301 caggttgtct aactccttcc ttttcggtta gagcggatgt ggggggaggg cgtgaatgta 
     3361 agcgtgacat aactaattac atgactcgag gtcgacggta tcgataagct tgcatgcctg 
     3421 caggtctcag cccatcttct tccagatggt gagcgaggcg gtgaggactc cagccacaaa 
     3481 gatggtcact gtctgccatg tgggggtccc gaagtaggag aggaggcctt cccagccacc 
     3541 ctggtcttgg atccagacaa gcagccgctc acggaggaag tccagtgtcc agcccatgat 
     3601 ggttctgatc agctcgggca ctttagtgca cagggccttg agcaccagtt tgctagcaaa 
     3661 gtagaagagg gcaaccacgc ggccccagtt gaagttgcca tcagcaaaca tgtcagctgc 
     3721 cacccggaag aagacctctc ggggggagtc cgtgtccacg tcagcaatca tcctctgcag 
     3781 ctccatattg ctatccagtt catctccaat tcgccggaga cactcgctca gcttcttggt 
     3841 ggacgcatcc tggggcggct gctccaaggt cagctcaggt gtctccccag ccatcctccc 
     3901 tgctcgatcc tggatgaaac cctgtagcaa aaaggcccct gtcttcatga tctgttcaga 
     3961 gctggtgggc ccgccgctcc caagctgctc cccggacccg tccatgactc tagttctaga 
     4021 tatagttttt tctccttgac gttaaagtat agaggtatat taacaatttt ttgttgatac 
     4081 ttttattaca tttgaataag aagtaataca aaccgaaaat gttgaaagta ttagttaaag 
     4141 tggttatgca gtttttgcat ttatatatct gttaatagat caaaaatcat cgcttcgctg 
     4201 attaattacc ccagaaataa ggctaaaaaa ctaatcgcat tatcatccta tggttgttaa 
     4261 tttgattcgt tcatttgaag gtttgtgggg ccaggttact gccaattttt cctcttcata 
     4321 accataaaag ctagtattgt agaatcttta ttgttcggag cagtgcggcg cgaggcacat 
     4381 ctgcgtttca ggaacgcgac cggtgaagac gaggacgcac ggaggagagt cttccttcgg 
     4441 agggctgtca gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt 
     4501 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 
     4561 taggagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagg taactcacat 
     4621 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 
     4681 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 
     4741 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 
     4801 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 
     4861 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 
     4921 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 
     4981 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 
     5041 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 
     5101 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 
     5161 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 
     5221 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 
     5281 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 
     5341 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 
     5401 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 
     5461 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 
     5521 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 
     5581 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 
     5641 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 
     5701 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 
     5761 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 
     5821 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 
     5881 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 
     5941 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 
     6001 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 
     6061 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 
     6121 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 
     6181 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 
     6241 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 
     6301 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 
     6361 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 
     6421 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 
     6481 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 
     6541 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 
     6601 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 
     6661 aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt 
     6721 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt 
     6781 taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa 
     6841 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgagagcgc 
     6901 tattttacca acaaagaatc tatacttctt ttttgttcta caaaaatgca tcccgagagc 
     6961 gctatttttc taacaaagca tcttagatta ctttttttct cctttgtgcg ctctataatg 
     7021 cagtctcttg ataacttttt gcactgtagg tccgttaagg ttagaagaag gctactttgg 
     7081 tgtctatttt ctcttccata aaaaaagcct gactccactt cccgcgttta ctgattacta 
     7141 gcgaagctgc gggtgcattt tttcaagata aaggcatccc cgattatatt ctataccgat 
     7201 gtggattgcg catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag 
     7261 aaaattatga acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca 
     7321 ttttcgtatt gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa 
     7381 gagtaatact agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg 
     7441 agcgaaaggt ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga 
     7501 tacttttgag caatgtttgt ggaagcggta ttcgcaatat tttagtagct cgttacagtc 
     7561 cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc 
     7621 tctgaagttc ctatactttc tagagaatag gaacttcgga ataggaactt caaagcgttt 
     7681 ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc acatacagct cactgttcac 
     7741 gtcgcaccta tatctgcgtg ttgcctgtat atatatatac atgagaagaa cggcatagtg 
     7801 cgtgtttatg cttaaatgcg tacttatatg cgtctattta tgtaggatga aaggtagtct 
     7861 agtacctcct gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta 
     7921 ccctttagct gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct 
     7981 atcatttcct ttgatattgg atcatactaa gaaaccatta ttatcatgac attaacctat 
     8041 aaaaataggc gtatcacgag gccctttcgt c 
//
