LOCUS       pAS2-hOMI-S306A         9788 bp    DNA     circular     11-JAN-2008
DEFINITION  LMBP 4578.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4578
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 11-JAN-2008
            
COMMENT     VNTDATE|457789485|
COMMENT     VNTNAME|pAS2-hOMI-S306A|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAS2-hOMI-S306A" 0 9788 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 21 22 62 29 30 32 33 43) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "BglI" "EcoRI" "EcoRV" "NaeI" "NdeI" "NheI" "SalI" "SfiI" 
COMMENT         "SpeI" "SphI" "StuI" "XbaI" "Csp45I") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 9788 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 3 3134 0 7384 0 8992 0 
COMMENT            0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 3 3951 0 5946 0 7043 0 0 
COMMENT            "") 
COMMENT        #2=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 3 2169 0 3563 0 4769 0 0 
COMMENT            "") 
COMMENT        #3=(CRSite (CStringList) "NaeI" "gccggc" 4 0 2 2989 0 6917 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 5998 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 5982 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 7400 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "SfiI" "ggccnnnnnggcc" 9 0 1 7384 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SpeI" "actagt" 2 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "SphI" "gcatgc" 6 0 4 5077 0 5533 0 6211 0 7618 
COMMENT            0 0 "") #10=(CRSite (CStringList) "StuI" "aggcct" 4 0 1 6282 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "XbaI" "tctaga" 2 0 3 981 0 1968 0 4763 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "Csp45I" "ttcgaa" 3 0 1 7292 0 0 "")) 
COMMENT       (CObList 
COMMENT        #13=(CFSignal (CObList) "FLP terminator" 43 0 1 1060 1164 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(1060..1164)") 
COMMENT        #14=(CFSignal (CObList) "CYC1 terminator" 43 0 1 3312 3586 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3312..3586)") 
COMMENT        #15=(CFSignal (CObList) "ADH1 promoter" 29 0 0 5075 5478 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5075..5478") 
COMMENT        #16=(CFSignal (CObList) "GALbd" 4 0 0 5501 5941 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5501..5941") 
COMMENT        #17=(CFSignal (CObList) "HA epitope" 22 0 0 5957 5983 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5957..5983") 
COMMENT        #18=(CFSignal (CObList) "2mu ori" 33 0 1 2 982 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2..982)") 
COMMENT        #19=(CFSignal (CObList) "TRP1" 4 0 0 1884 2558 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1884..2558") 
COMMENT        #20=(CFSignal (CObList) "f1 ori" 33 0 1 2661 3116 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2661..3116)") 
COMMENT        #21=(CFSignal (CObList) "CYH2" 4 0 1 3855 4304 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3855..4304)") 
COMMENT        #22=(CFSignal (CObList) "hOMI mutant" 4 0 0 5999 7375 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5999..7375") 
COMMENT        #23=(CFSignal (CObList) "lac promoter" 30 0 1 7680 7763 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(7680..7763)") 
COMMENT        #24=(CFSignal (CObList) "ADH1 terminator" 43 0 0 7426 7527 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "7426..7527") 
COMMENT        #25=(CFSignal (CObList) "pMB1 ori" 33 0 1 7855 8257 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(7855..8257)") 
COMMENT        #26=(CFSignal (CObList) "ampicillin" 4 0 1 8798 9658 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(8798..9658)") 
COMMENT        #27=(CFSignal (CObList) "S306A" 62 0 0 6914 6919 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #28=(CFSignal (CObList) "LMBP 4578" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #29=(CFSignal (CObList) "Last modified: 11-JAN-2008" 2 0 0 0 0 0 
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COMMENT                    2) 5 "" 0 4) 
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COMMENT                          "DNA Plasmid 'pAS2-hOMI-S306A'" 1) 
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COMMENT                          1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 09/18/07 11:44" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 01/11/08 03:34" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 9788 bp" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #43=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 18-SEP-2007" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #46=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
COMMENT                          1))) "Original Author") 
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COMMENT                                 "" 1))) "LMBP 4578") 
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COMMENT                                        "   5501..5941" 1))) "GALbd") 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5999..7375" 1))) "hOMI mutant") 
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COMMENT                                        "Start: 8798  End: 9658 (Complementary)" 
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COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(8798..9658)" 1))) 
COMMENT                                 "ampicillin"))) "CDS (5 total)") 
COMMENT                     #75=(CFolderPar 
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COMMENT                                        "Start: 5957  End: 5983" 1) 
COMMENT                                   #78=(CLinePar 
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COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5957..5983" 1))) "HA epitope"))) 
COMMENT                          "Misc. Marker (1 total)") 
COMMENT                     #80=(CFolderPar 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5075..5478" 1))) "ADH1 promoter"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
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COMMENT                                        "Start: 7680  End: 7763 (Complementary)" 
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COMMENT                                   #88=(CLinePar 
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COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(7680..7763)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
COMMENT                     #90=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                   #93=(CLinePar 
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COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2..982)" 1))) "2mu ori") 
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COMMENT                                        "   complement(2661..3116)" 1))) 
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COMMENT                                           "WT: TCT.GGA" 1))) "S306A"))) 
COMMENT                           "Mutation (1 total)"))) "Feature Map") 
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COMMENT                            8 5 "GGCCNNNNNGGCC" "CCGGNNNNNCCGG") 
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FEATURES             Location/Qualifiers
     terminator      complement(1060..1164)
                     /vntifkey="43"
                     /label=FLP\terminator
     terminator      complement(3312..3586)
                     /vntifkey="43"
                     /label=CYC1\terminator
     promoter        5075..5478
                     /vntifkey="29"
                     /label=ADH1\promoter
     CDS             5501..5941
                     /vntifkey="4"
                     /label=GALbd
     misc_marker     5957..5983
                     /vntifkey="22"
                     /label=HA\epitope
     rep_origin      complement(2..982)
                     /vntifkey="33"
                     /label=2mu\ori
     CDS             1884..2558
                     /vntifkey="4"
                     /label=TRP1
     rep_origin      complement(2661..3116)
                     /vntifkey="33"
                     /label=f1\ori
     CDS             complement(3855..4304)
                     /vntifkey="4"
                     /label=CYH2
     CDS             5999..7375
                     /vntifkey="4"
                     /label=hOMI\mutant
     promoter        complement(7680..7763)
                     /vntifkey="30"
                     /label=lac\promoter
     terminator      7426..7527
                     /vntifkey="43"
                     /label=ADH1\terminator
     rep_origin      complement(7855..8257)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(8798..9658)
                     /vntifkey="4"
                     /label=ampicillin
     mutation        6914..6919
                     /vntifkey="62"
                     /label=S306A
                     /note="WT: TCT.GGA"
BASE COUNT     2606 a      2151 c      2315 g      2716 t 
ORIGIN
        1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 
       61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 
      121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 
      181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 
      241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 
      301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 
      361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 
      421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 
      481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 
      541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 
      601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 
      661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 
      721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 
      781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 
      841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 
      901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 
      961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 
     1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 
     1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 
     1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 
     1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 
     1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 
     1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 
     1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 
     1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 
     1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 
     1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 
     1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 
     1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 
     1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 
     1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 
     1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 
     1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 
     1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 
     2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 
     2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 
     2161 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg 
     2221 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag 
     2281 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg 
     2341 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct 
     2401 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa 
     2461 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta 
     2521 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat 
     2581 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa 
     2641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 
     2701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 
     2761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 
     2821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 
     2881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 
     2941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 
     3001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 
     3061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg 
     3121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 
     3181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 
     3241 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga 
     3301 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt 
     3361 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata 
     3421 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag 
     3481 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc 
     3541 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt 
     3601 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca 
     3661 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 
     3721 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga 
     3781 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct 
     3841 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct 
     3901 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg aattctaccc 
     3961 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta 
     4021 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc 
     4081 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc 
     4141 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg 
     4201 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag 
     4261 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat 
     4321 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag 
     4381 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata 
     4441 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg 
     4501 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat 
     4561 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt 
     4621 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc 
     4681 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca 
     4741 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg 
     4801 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt 
     4861 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca 
     4921 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa 
     4981 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt 
     5041 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt 
     5101 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac 
     5161 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 
     5221 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc 
     5281 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt 
     5341 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat 
     5401 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag 
     5461 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat 
     5521 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa 
     5581 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc 
     5641 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct 
     5701 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca 
     5761 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt 
     5821 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat 
     5881 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc 
     5941 gccggaattc atggcttacc catacgatgt tccagattac gctagcttgg gtggtcatat 
     6001 ggctgcgccg agggcggggc ggggtgcagg ctggagcctt cgggcatggc gggctttggg 
     6061 gggcattcgc tgggggagga gaccccgttt gacccctgac ctccgggccc tgctgacgtc 
     6121 aggaacttct gacccccggg cccgagtgac ttatgggacc cccagtctct gggcccggtt 
     6181 gtctgttggg gtcactgaac cccgagcatg cctgacgtct gggaccccgg gtccccgggc 
     6241 acaactgact gcggtgaccc cagataccag gacccgggag gcctcagaga actctggaac 
     6301 ccgttcgcgc gcgtggctgg cggtggcgct gggcgctggg ggggcagtgc tgttgttgtt 
     6361 gtggggcggg ggtcggggtc ctccggccgt cctcgccgcc gtccctagcc cgccgcccgc 
     6421 ttctccccgg agtcagtaca acttcatcgc agatgtggtg gagaagacag cacctgccgt 
     6481 ggtctatatc gagatcctgg accggcaccc tttcttgggc cgcgaggtcc ctatctcgaa 
     6541 cggctcagga ttcgtggtgg ctgccgatgg gctcattgtc accaacgccc atgtggtggc 
     6601 tgatcggcgc agagtccgtg tgagactgct aagcggcgac acgtatgagg ccgtggtcac 
     6661 agctgtggat cccgtggcag acatcgcaac gctgaggatt cagactaagg agcctctccc 
     6721 cacgctgcct ctgggacgct cagctgatgt ccggcaaggg gagtttgttg ttgccatggg 
     6781 aagtcccttt gcactgcaga acacgatcac atccggcatt gttagctctg ctcagcgtcc 
     6841 agccagagac ctgggactcc cccaaaccaa tgtggaatac attcaaactg atgcagctat 
     6901 tgattttgga aacgccggcg gtcccctggt taacctggat ggggaggtga ttggagtgaa 
     6961 caccatgaag gtcacagctg gaatctcctt tgccatccct tctgatcgtc ttcgagagtt 
     7021 tctgcatcgt ggggaaaaga agaattcctc ctccggaatc agtgggtccc agcggcgcta 
     7081 cattggggtg atgatgctga ccctgagtcc cagcatcctt gctgaactac agcttcgaga 
     7141 accaagcttt cccgatgttc agcatggtgt actcatccat aaagtcatcc tgggctcccc 
     7201 tgcacaccgg gctggtctgc ggcctggtga tgtgattttg gccattgggg agcagatggt 
     7261 acaaaatgct gaagatgttt atgaagctgt tcgaacccaa tcccagttgg cagtgcagat 
     7321 ccggcgggga cgagaaacac tgaccttata tgtgacccct gaggtcacag aatgaggcca 
     7381 tggaggcccc ggggatccgt cgacctgcag ccaagctaat tccgggcgaa tttcttatga 
     7441 tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt 
     7501 gactcttagg ttttaaaacg aaaattcttg ttcttgagta actctttcct gtaggtcagg 
     7561 ttgctttctc aggtatagca tgaggtcgct cttattgacc acacctctac cggcatgcaa 
     7621 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 
     7681 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgaggt 
     7741 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 
     7801 agctggatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 
     7861 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 
     7921 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 
     7981 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 
     8041 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 
     8101 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 
     8161 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 
     8221 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 
     8281 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 
     8341 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 
     8401 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 
     8461 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 
     8521 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 
     8581 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 
     8641 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 
     8701 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 
     8761 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 
     8821 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 
     8881 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 
     8941 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 
     9001 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 
     9061 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 
     9121 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 
     9181 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 
     9241 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 
     9301 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 
     9361 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 
     9421 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 
     9481 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 
     9541 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 
     9601 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 
     9661 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 
     9721 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 
     9781 tgccacct 
//
