ID pAS2-hOMI-S306A standard; circular DNA; ; 9788 BP. DE LMBP 4578. CC Accession number: LMBP 4578 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium). CC Last modified: 11-JAN-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT terminator complement(1060..1164) FT /vntifkey="43" FT /label=FLP\terminator FT terminator complement(3312..3586) FT /vntifkey="43" FT /label=CYC1\terminator FT promoter 5075..5478 FT /vntifkey="29" FT /label=ADH1\promoter FT CDS 5501..5941 FT /vntifkey="4" FT /label=GALbd FT misc_marker 5957..5983 FT /vntifkey="22" FT /label=HA\epitope FT rep_origin complement(2..982) FT /vntifkey="33" FT /label=2mu\ori FT CDS 1884..2558 FT /vntifkey="4" FT /label=TRP1 FT rep_origin complement(2661..3116) FT /vntifkey="33" FT /label=f1\ori FT CDS complement(3855..4304) FT /vntifkey="4" FT /label=CYH2 FT CDS 5999..7375 FT /vntifkey="4" FT /label=hOMI\mutant FT promoter complement(7680..7763) FT /vntifkey="30" FT /label=lac\promoter FT terminator 7426..7527 FT /vntifkey="43" FT /label=ADH1\terminator FT rep_origin complement(7855..8257) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(8798..9658) FT /vntifkey="4" FT /label=ampicillin FT mutation 6914..6919 FT /vntifkey="62" FT /label=S306A FT /note="WT: TCT.GGA" SQ Sequence 9788 BP; 2606 A; 2151 C; 2315 G; 2716 t; gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 60 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 120 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 180 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 240 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 300 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 360 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 420 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 480 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 540 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 600 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 660 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 720 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 780 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 840 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 900 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 960 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 1020 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 1080 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 1140 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 1200 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 1260 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 1320 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 1380 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 1440 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 1500 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 1560 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 1620 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 1680 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 1740 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 1800 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 1860 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 1920 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 1980 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 2040 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 2100 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 2160 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg 2220 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag 2280 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg 2340 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct 2400 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa 2460 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta 2520 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat 2580 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa 2640 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 2700 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 2760 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 2820 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 2880 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 2940 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 3000 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 3060 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg 3120 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 3180 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 3240 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga 3300 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt 3360 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata 3420 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag 3480 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc 3540 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt 3600 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca 3660 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 3720 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga 3780 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct 3840 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct 3900 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg aattctaccc 3960 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta 4020 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc 4080 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc 4140 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg 4200 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag 4260 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat 4320 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag 4380 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata 4440 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg 4500 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat 4560 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt 4620 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc 4680 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca 4740 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg 4800 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt 4860 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca 4920 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa 4980 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt 5040 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt 5100 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac 5160 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 5220 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc 5280 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt 5340 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat 5400 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag 5460 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat 5520 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa 5580 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc 5640 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct 5700 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca 5760 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt 5820 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat 5880 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc 5940 gccggaattc atggcttacc catacgatgt tccagattac gctagcttgg gtggtcatat 6000 ggctgcgccg agggcggggc ggggtgcagg ctggagcctt cgggcatggc gggctttggg 6060 gggcattcgc tgggggagga gaccccgttt gacccctgac ctccgggccc tgctgacgtc 6120 aggaacttct gacccccggg cccgagtgac ttatgggacc cccagtctct gggcccggtt 6180 gtctgttggg gtcactgaac cccgagcatg cctgacgtct gggaccccgg gtccccgggc 6240 acaactgact gcggtgaccc cagataccag gacccgggag gcctcagaga actctggaac 6300 ccgttcgcgc gcgtggctgg cggtggcgct gggcgctggg ggggcagtgc tgttgttgtt 6360 gtggggcggg ggtcggggtc ctccggccgt cctcgccgcc gtccctagcc cgccgcccgc 6420 ttctccccgg agtcagtaca acttcatcgc agatgtggtg gagaagacag cacctgccgt 6480 ggtctatatc gagatcctgg accggcaccc tttcttgggc cgcgaggtcc ctatctcgaa 6540 cggctcagga ttcgtggtgg ctgccgatgg gctcattgtc accaacgccc atgtggtggc 6600 tgatcggcgc agagtccgtg tgagactgct aagcggcgac acgtatgagg ccgtggtcac 6660 agctgtggat cccgtggcag acatcgcaac gctgaggatt cagactaagg agcctctccc 6720 cacgctgcct ctgggacgct cagctgatgt ccggcaaggg gagtttgttg ttgccatggg 6780 aagtcccttt gcactgcaga acacgatcac atccggcatt gttagctctg ctcagcgtcc 6840 agccagagac ctgggactcc cccaaaccaa tgtggaatac attcaaactg atgcagctat 6900 tgattttgga aacgccggcg gtcccctggt taacctggat ggggaggtga ttggagtgaa 6960 caccatgaag gtcacagctg gaatctcctt tgccatccct tctgatcgtc ttcgagagtt 7020 tctgcatcgt ggggaaaaga agaattcctc ctccggaatc agtgggtccc agcggcgcta 7080 cattggggtg atgatgctga ccctgagtcc cagcatcctt gctgaactac agcttcgaga 7140 accaagcttt cccgatgttc agcatggtgt actcatccat aaagtcatcc tgggctcccc 7200 tgcacaccgg gctggtctgc ggcctggtga tgtgattttg gccattgggg agcagatggt 7260 acaaaatgct gaagatgttt atgaagctgt tcgaacccaa tcccagttgg cagtgcagat 7320 ccggcgggga cgagaaacac tgaccttata tgtgacccct gaggtcacag aatgaggcca 7380 tggaggcccc ggggatccgt cgacctgcag ccaagctaat tccgggcgaa tttcttatga 7440 tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt 7500 gactcttagg ttttaaaacg aaaattcttg ttcttgagta actctttcct gtaggtcagg 7560 ttgctttctc aggtatagca tgaggtcgct cttattgacc acacctctac cggcatgcaa 7620 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 7680 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgaggt 7740 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 7800 agctggatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 7860 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 7920 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 7980 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 8040 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 8100 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 8160 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 8220 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 8280 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 8340 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 8400 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 8460 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 8520 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 8580 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 8640 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 8700 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 8760 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 8820 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 8880 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 8940 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 9000 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 9060 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 9120 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 9180 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 9240 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 9300 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 9360 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 9420 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 9480 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 9540 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 9600 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 9660 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 9720 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 9780 tgccacct 9788 //