LOCUS       pAS2-AVPS-hOMI-S306A        9392 bp    DNA     circular     11-JAN-2008
DEFINITION  LMBP 4579.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4579
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 11-JAN-2008
            
COMMENT     VNTDATE|457787697|
COMMENT     VNTNAME|pAS2-AVPS-hOMI-S306A|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAS2-AVPS-hOMI-S306A" 0 9392 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 21 22 62 29 30 32 33 43) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "BglI" "EcoRI" "EcoRV" "NdeI" "NheI" "SalI" "SfiI" "SpeI" 
COMMENT         "SphI" "StuI" "XbaI" "Csp45I" "NaeI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 9392 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 3 3134 0 6988 0 8596 0 
COMMENT            0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 3 3951 0 5946 0 6647 0 0 
COMMENT            "") 
COMMENT        #2=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 3 2169 0 3563 0 4769 0 0 
COMMENT            "") #3=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 5998 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 5982 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 7004 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "SfiI" "ggccnnnnnggcc" 9 0 1 6988 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "SpeI" "actagt" 2 0 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SphI" "gcatgc" 6 0 3 5077 0 5533 0 7222 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "StuI" "aggcct" 4 0 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "XbaI" "tctaga" 2 0 3 981 0 1968 0 4763 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "Csp45I" "ttcgaa" 3 0 1 6896 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "NaeI" "gccggc" 4 0 2 2989 0 6521 0 0 "")) 
COMMENT       (CObList 
COMMENT        #13=(CFSignal (CObList) "FLP terminator" 43 0 1 1060 1164 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #14=(CFSignal (CObList) "CYC1 terminator" 43 0 1 3312 3586 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3312..3586)") 
COMMENT        #15=(CFSignal (CObList) "ADH1 promoter" 29 0 0 5075 5478 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5075..5478") 
COMMENT        #16=(CFSignal (CObList) "GALbd" 4 0 0 5501 5941 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5501..5941") 
COMMENT        #17=(CFSignal (CObList) "HA epitope" 22 0 0 5957 5983 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5957..5983") 
COMMENT        #18=(CFSignal (CObList) "2mu ori" 33 0 1 2 982 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2..982)") 
COMMENT        #19=(CFSignal (CObList) "TRP1" 4 0 0 1884 2558 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1884..2558") 
COMMENT        #20=(CFSignal (CObList) "f1 ori" 33 0 1 2661 3116 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2661..3116)") 
COMMENT        #21=(CFSignal (CObList) "CYH2" 4 0 1 3855 4304 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3855..4304)") 
COMMENT        #22=(CFSignal (CObList) "mature hOMI mutant" 4 0 0 5999 6979 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5999..6979") 
COMMENT        #23=(CFSignal (CObList) "lac promoter" 30 0 1 7284 7367 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #24=(CFSignal (CObList) "ADH1 terminator" 43 0 0 7030 7131 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "6051..6152") 
COMMENT        #25=(CFSignal (CObList) "pMB1 ori" 33 0 1 7459 7861 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(6480..6882)") 
COMMENT        #26=(CFSignal (CObList) "ampicillin" 4 0 1 8402 9262 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(7423..8283)") 
COMMENT        #27=(CFSignal (CObList) "S306A" 62 0 0 6518 6523 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
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COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #29=(CFSignal (CObList) "Last modified: 11-JAN-2008" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pAS2-AVPS-hOMI-S306A'" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4579" 
COMMENT                          1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 09/18/07 11:14" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 01/11/08 03:46" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 9392 bp" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #43=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 18-SEP-2007" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #44=(CRefLinePar 
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COMMENT                   0 0) 
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COMMENT                                 "" 1))) "LMBP 4579") 
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COMMENT                                 "mature hOMI mutant") 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(7423..8283)" 1))) 
COMMENT                                 "ampicillin"))) "CDS (5 total)") 
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COMMENT                                        "Start: 5957  End: 5983" 1) 
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COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5957..5983" 1))) "HA epitope"))) 
COMMENT                          "Misc. Marker (1 total)") 
COMMENT                     #77=(CFolderPar 
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COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5075..5478" 1))) "ADH1 promoter"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7284  End: 7367 (Complementary)" 
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COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                                  (CObjectList 
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COMMENT                                        "Start: 2     End: 982  (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #88=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2..982)" 1))) "2mu ori") 
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COMMENT                                   #92=(CLinePar 
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COMMENT                                        "   complement(2661..3116)" 1))) 
COMMENT                                 "f1 ori") 
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COMMENT                                   #97=(CLinePar 
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COMMENT                                        "   complement(6480..6882)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (3 total)") 
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COMMENT                                     #112=(CLinePar 
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COMMENT                                           "WT: TCT.GGA" 1))) "S306A"))) 
COMMENT                           "Mutation (1 total)"))) "Feature Map") 
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COMMENT                                            " N1: 5998 " 1))))) "NdeI: 1 site") 
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COMMENT                                            " N1: 6988 " 1))))) "SfiI: 1 site") 
COMMENT                            8 5 "GGCCNNNNNGGCC" "CCGGNNNNNCCGG") 
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COMMENT                            3 3 "GCCGGC" "CGGCCG") 
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FEATURES             Location/Qualifiers
     terminator      complement(1060..1164)
                     /vntifkey="43"
                     /label=FLP\terminator
     terminator      complement(3312..3586)
                     /vntifkey="43"
                     /label=CYC1\terminator
     promoter        5075..5478
                     /vntifkey="29"
                     /label=ADH1\promoter
     CDS             5501..5941
                     /vntifkey="4"
                     /label=GALbd
     misc_marker     5957..5983
                     /vntifkey="22"
                     /label=HA\epitope
     rep_origin      complement(2..982)
                     /vntifkey="33"
                     /label=2mu\ori
     CDS             1884..2558
                     /vntifkey="4"
                     /label=TRP1
     rep_origin      complement(2661..3116)
                     /vntifkey="33"
                     /label=f1\ori
     CDS             complement(3855..4304)
                     /vntifkey="4"
                     /label=CYH2
     CDS             5999..6979
                     /vntifkey="4"
                     /label=mature\hOMI\mutant
     promoter        complement(7284..7367)
                     /vntifkey="30"
                     /label=lac\promoter
     terminator      7030..7131
                     /vntifkey="43"
                     /label=ADH1\terminator
     rep_origin      complement(7459..7861)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(8402..9262)
                     /vntifkey="4"
                     /label=ampicillin
     mutation        6518..6523
                     /vntifkey="62"
                     /label=S306A
                     /note="WT: TCT.GGA"
BASE COUNT     2561 a      2028 c      2158 g      2645 t 
ORIGIN
        1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 
       61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 
      121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 
      181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 
      241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 
      301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 
      361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 
      421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 
      481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 
      541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 
      601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 
      661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 
      721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 
      781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 
      841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 
      901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 
      961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 
     1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 
     1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 
     1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 
     1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 
     1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 
     1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 
     1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 
     1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 
     1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 
     1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 
     1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 
     1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 
     1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 
     1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 
     1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 
     1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 
     1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 
     2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 
     2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 
     2161 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg 
     2221 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag 
     2281 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg 
     2341 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct 
     2401 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa 
     2461 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta 
     2521 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat 
     2581 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa 
     2641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 
     2701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 
     2761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 
     2821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 
     2881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 
     2941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 
     3001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 
     3061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg 
     3121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 
     3181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 
     3241 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga 
     3301 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt 
     3361 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata 
     3421 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag 
     3481 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc 
     3541 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt 
     3601 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca 
     3661 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 
     3721 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga 
     3781 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct 
     3841 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct 
     3901 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg aattctaccc 
     3961 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta 
     4021 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc 
     4081 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc 
     4141 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg 
     4201 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag 
     4261 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat 
     4321 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag 
     4381 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata 
     4441 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg 
     4501 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat 
     4561 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt 
     4621 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc 
     4681 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca 
     4741 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg 
     4801 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt 
     4861 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca 
     4921 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa 
     4981 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt 
     5041 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt 
     5101 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac 
     5161 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 
     5221 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc 
     5281 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt 
     5341 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat 
     5401 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag 
     5461 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat 
     5521 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa 
     5581 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc 
     5641 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct 
     5701 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca 
     5761 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt 
     5821 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat 
     5881 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc 
     5941 gccggaattc atggcttacc catacgatgt tccagattac gctagcttgg gtggtcatat 
     6001 ggccgtccct agcccgccgc ccgcttctcc ccggagtcag tacaacttca tcgcagatgt 
     6061 ggtggagaag acagcacctg ccgtggtcta tatcgagatc ctggaccggc accctttctt 
     6121 gggccgcgag gtccctatct cgaacggctc aggattcgtg gtggctgccg atgggctcat 
     6181 tgtcaccaac gcccatgtgg tggctgatcg gcgcagagtc cgtgtgagac tgctaagcgg 
     6241 cgacacgtat gaggccgtgg tcacagctgt ggatcccgtg gcagacatcg caacgctgag 
     6301 gattcagact aaggagcctc tccccacgct gcctctggga cgctcagctg atgtccggca 
     6361 aggggagttt gttgttgcca tgggaagtcc ctttgcactg cagaacacga tcacatccgg 
     6421 cattgttagc tctgctcagc gtccagccag agacctggga ctcccccaaa ccaatgtgga 
     6481 atacattcaa actgatgcag ctattgattt tggaaacgcc ggcggtcccc tggttaacct 
     6541 ggatggggag gtgattggag tgaacaccat gaaggtcaca gctggaatct cctttgccat 
     6601 cccttctgat cgtcttcgag agtttctgca tcgtggggaa aagaagaatt cctcctccgg 
     6661 aatcagtggg tcccagcggc gctacattgg ggtgatgatg ctgaccctga gtcccagcat 
     6721 ccttgctgaa ctacagcttc gagaaccaag ctttcccgat gttcagcatg gtgtactcat 
     6781 ccataaagtc atcctgggct cccctgcaca ccgggctggt ctgcggcctg gtgatgtgat 
     6841 tttggccatt ggggagcaga tggtacaaaa tgctgaagat gtttatgaag ctgttcgaac 
     6901 ccaatcccag ttggcagtgc agatccggcg gggacgagaa acactgacct tatatgtgac 
     6961 ccctgaggtc acagaatgag gccatggagg ccccggggat ccgtcgacct gcagccaagc 
     7021 taattccggg cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa 
     7081 ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt cttgttcttg 
     7141 agtaactctt tcctgtaggt caggttgctt tctcaggtat agcatgaggt cgctcttatt 
     7201 gaccacacct ctaccggcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt 
     7261 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 
     7321 cctggggtgc ctaatgagtg aggtaactca cattaattgc gttgcgctca ctgcccgctt 
     7381 tccagtcggg aaacctgtcg tgccagctgg attaatgaat cggccaacgc gcggggagag 
     7441 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 
     7501 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 
     7561 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 
     7621 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 
     7681 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 
     7741 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 
     7801 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 
     7861 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 
     7921 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 
     7981 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 
     8041 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 
     8101 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 
     8161 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 
     8221 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 
     8281 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 
     8341 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 
     8401 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 
     8461 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 
     8521 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 
     8581 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 
     8641 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 
     8701 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 
     8761 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 
     8821 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 
     8881 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 
     8941 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 
     9001 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 
     9061 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 
     9121 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 
     9181 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 
     9241 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 
     9301 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 
     9361 ttccgcgcac atttccccga aaagtgccac ct 
//
