ID pAS2-AVPS-hOMI-S306A standard; circular DNA; ; 9392 BP. DE LMBP 4579. CC Accession number: LMBP 4579 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 11-JAN-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT terminator complement(1060..1164) FT /vntifkey="43" FT /label=FLP\terminator FT terminator complement(3312..3586) FT /vntifkey="43" FT /label=CYC1\terminator FT promoter 5075..5478 FT /vntifkey="29" FT /label=ADH1\promoter FT CDS 5501..5941 FT /vntifkey="4" FT /label=GALbd FT misc_marker 5957..5983 FT /vntifkey="22" FT /label=HA\epitope FT rep_origin complement(2..982) FT /vntifkey="33" FT /label=2mu\ori FT CDS 1884..2558 FT /vntifkey="4" FT /label=TRP1 FT rep_origin complement(2661..3116) FT /vntifkey="33" FT /label=f1\ori FT CDS complement(3855..4304) FT /vntifkey="4" FT /label=CYH2 FT CDS 5999..6979 FT /vntifkey="4" FT /label=mature\hOMI\mutant FT promoter complement(7284..7367) FT /vntifkey="30" FT /label=lac\promoter FT terminator 7030..7131 FT /vntifkey="43" FT /label=ADH1\terminator FT rep_origin complement(7459..7861) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(8402..9262) FT /vntifkey="4" FT /label=ampicillin FT mutation 6518..6523 FT /vntifkey="62" FT /label=S306A FT /note="WT: TCT.GGA" SQ Sequence 9392 BP; 2561 A; 2028 C; 2158 G; 2645 t; gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 60 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 120 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 180 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 240 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 300 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 360 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 420 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 480 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 540 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 600 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 660 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 720 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 780 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 840 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 900 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 960 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 1020 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 1080 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 1140 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 1200 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 1260 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 1320 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 1380 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 1440 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 1500 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 1560 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 1620 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 1680 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 1740 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 1800 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 1860 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 1920 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 1980 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 2040 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 2100 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 2160 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg 2220 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag 2280 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg 2340 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct 2400 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa 2460 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta 2520 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat 2580 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa 2640 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 2700 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 2760 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 2820 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 2880 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 2940 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 3000 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 3060 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg 3120 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 3180 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 3240 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga 3300 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt 3360 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata 3420 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag 3480 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc 3540 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt 3600 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca 3660 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 3720 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga 3780 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct 3840 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct 3900 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg aattctaccc 3960 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta 4020 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc 4080 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc 4140 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg 4200 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag 4260 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat 4320 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag 4380 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata 4440 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg 4500 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat 4560 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt 4620 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc 4680 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca 4740 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg 4800 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt 4860 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca 4920 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa 4980 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt 5040 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt 5100 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac 5160 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 5220 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc 5280 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt 5340 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat 5400 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag 5460 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat 5520 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa 5580 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc 5640 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct 5700 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca 5760 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt 5820 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat 5880 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc 5940 gccggaattc atggcttacc catacgatgt tccagattac gctagcttgg gtggtcatat 6000 ggccgtccct agcccgccgc ccgcttctcc ccggagtcag tacaacttca tcgcagatgt 6060 ggtggagaag acagcacctg ccgtggtcta tatcgagatc ctggaccggc accctttctt 6120 gggccgcgag gtccctatct cgaacggctc aggattcgtg gtggctgccg atgggctcat 6180 tgtcaccaac gcccatgtgg tggctgatcg gcgcagagtc cgtgtgagac tgctaagcgg 6240 cgacacgtat gaggccgtgg tcacagctgt ggatcccgtg gcagacatcg caacgctgag 6300 gattcagact aaggagcctc tccccacgct gcctctggga cgctcagctg atgtccggca 6360 aggggagttt gttgttgcca tgggaagtcc ctttgcactg cagaacacga tcacatccgg 6420 cattgttagc tctgctcagc gtccagccag agacctggga ctcccccaaa ccaatgtgga 6480 atacattcaa actgatgcag ctattgattt tggaaacgcc ggcggtcccc tggttaacct 6540 ggatggggag gtgattggag tgaacaccat gaaggtcaca gctggaatct cctttgccat 6600 cccttctgat cgtcttcgag agtttctgca tcgtggggaa aagaagaatt cctcctccgg 6660 aatcagtggg tcccagcggc gctacattgg ggtgatgatg ctgaccctga gtcccagcat 6720 ccttgctgaa ctacagcttc gagaaccaag ctttcccgat gttcagcatg gtgtactcat 6780 ccataaagtc atcctgggct cccctgcaca ccgggctggt ctgcggcctg gtgatgtgat 6840 tttggccatt ggggagcaga tggtacaaaa tgctgaagat gtttatgaag ctgttcgaac 6900 ccaatcccag ttggcagtgc agatccggcg gggacgagaa acactgacct tatatgtgac 6960 ccctgaggtc acagaatgag gccatggagg ccccggggat ccgtcgacct gcagccaagc 7020 taattccggg cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa 7080 ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt cttgttcttg 7140 agtaactctt tcctgtaggt caggttgctt tctcaggtat agcatgaggt cgctcttatt 7200 gaccacacct ctaccggcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt 7260 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 7320 cctggggtgc ctaatgagtg aggtaactca cattaattgc gttgcgctca ctgcccgctt 7380 tccagtcggg aaacctgtcg tgccagctgg attaatgaat cggccaacgc gcggggagag 7440 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 7500 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 7560 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 7620 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 7680 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 7740 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 7800 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 7860 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 7920 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 7980 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 8040 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 8100 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 8160 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 8220 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 8280 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 8340 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 8400 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 8460 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 8520 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 8580 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 8640 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 8700 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 8760 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 8820 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 8880 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 8940 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 9000 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 9060 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 9120 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 9180 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 9240 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 9300 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 9360 ttccgcgcac atttccccga aaagtgccac ct 9392 //