LOCUS       pBlueBacmsGCB1-H105F        6936 bp    DNA     circular     24-NOV-2015
DEFINITION  LMBP 4643.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4643
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 24-NOV-2015
            
            
COMMENT     VNTNAME|pBlueBacmsGCB1-H105F|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBlueBacmsGCB1-H105F" 0 6936 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList 
COMMENT         #0=(CFragment 3 1 0 0 0 "" 0 "" "" "" "" "" "" "pBlueBachsGCA1His" "" 0 
COMMENT             0 1 138 1 138 1 138 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 4 0 0 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "BamHI" "" 1 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 3 1 0 0 0 "" 0 "" "" "" "" "" "" "msGCB1-H105F" "" 0 0 1 
COMMENT             1955 139 2093 2 1956 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "BamHI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "HindIII" "" 1 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #2=(CFragment 3 1 0 2 0 "" 0 "" "" "" "" "" "" "pBlueBachsGCA1His" "" 0 
COMMENT             0 1 4843 2094 6936 2274 7116 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "HindIII" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 4 1 0 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BglII" "EcoRV" "HaeII" "HindIII" "SacI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 6936 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #3=(CRSite (CStringList) "BglII" "agatct" 2 0 2 152 0 773 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 4 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2094 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 149 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 6 1424 0 2468 0 5162 0 
COMMENT            5743 0 6180 0 6474 0 0 "")) 
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COMMENT            (CStringList) 1 1 1 1 "506..508") 
COMMENT        #9=(CFSignal (CObList) "mGucy1b3 mutant" 4 0 0 194 2053 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "194..2053") 
COMMENT        #10=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2198 2272 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2198..2272") 
COMMENT        #11=(CFSignal (CObList) "V5 epitope" 22 0 0 2105 2146 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2105..2146") 
COMMENT        #12=(CFSignal (CObList) "His-tag" 22 0 0 2156 2173 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2156..2173") 
COMMENT        #13=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2198 2272 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2198..2272)") 
COMMENT        #14=(CFSignal (CObList) "ampicillin resistance" 4 0 0 3726 4586 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3726..4586") 
COMMENT        #15=(CFSignal (CObList) "pMB1 ori" 33 0 0 5127 5521 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5127..5521") 
COMMENT        #16=(CFSignal (CObList) "lacZ fragment" 4 0 1 5522 6634 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5522..6634)") 
COMMENT        #17=(CFSignal (CObList) "AcNPV-ETL promoter" 29 0 1 6635 6909 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(6635..6909)") 
COMMENT        #18=(CFSignal (CObList) "AcNPV-PH promoter" 29 0 0 7 95 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "7..95") 
COMMENT        #19=(CFSignal (CObList) "AcNPV ORF 1629 fragment" 4 0 1 2460 3338 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2460..3338)")) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                          "Last Modified: 11/24/15 09:44" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6936 bp" 1) 
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COMMENT                          "storage type: Constructed" 1) 
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COMMENT                          "Modification Date in the Original DB: 23-NOV-2015" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                 "" 1))) "LMBP 4643") 
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COMMENT                                 "ampicillin resistance") 
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FEATURES             Location/Qualifiers
     mutation        506..508
                     /vntifkey="62"
                     /label=H105F
                     /note="WT: CAT"
     CDS             194..2053
                     /vntifkey="4"
                     /label=mGucy1b3\mutant
     terminator      2198..2272
                     /vntifkey="43"
                     /label=SV40\polyA\early
     misc_marker     2105..2146
                     /vntifkey="22"
                     /label=V5\epitope
     misc_marker     2156..2173
                     /vntifkey="22"
                     /label=His-tag
     terminator      complement(2198..2272)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     CDS             complement(2460..3338)
                     /vntifkey="4"
                     /label=AcNPV\ORF\1629\fragment
     CDS             3726..4586
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      5127..5521
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(5522..6634)
                     /vntifkey="4"
                     /label=lacZ\fragment
     promoter        complement(6635..6909)
                     /vntifkey="29"
                     /label=AcNPV-ETL\promoter
     promoter        7..95
                     /vntifkey="29"
                     /label=AcNPV-PH\promoter
BASE COUNT     1789 a      1656 c      1697 g      1794 t 
ORIGIN
        1 gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt 
       61 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca 
      121 ccatcgggcg tgctagcgga tccgagctcg agatctgcag ctggtacccg ggtctagaat 
      181 tgcccttgac accatgtacg gtttcgtgaa ccatgccctg gagctgctgg tgatccgcaa 
      241 ttatggtccc gaggtgtggg aagacatcaa aaaagaggca cagctggatg aagaaggcca 
      301 gtttcttgtc agaataatat acgatgattc caaaacatat gacctggtgg ctgctgcaag 
      361 caaagtcctc aacctcaatg ctggcgaaat cctgcagatg tttgggaaga tgtttttcgt 
      421 cttctgccag gagtctggct atgataccat cttgcgtgtc ctgggatcta atgtcagaga 
      481 gtttttgcag aacctcgatg ccctcttcga tcatctggcc accatttacc ccgggatgcg 
      541 cgcgccttcc ttcaggtgca ccgatgcgga gaaaggcaaa gggctcatcc tgcactacta 
      601 ctcggaaaga gaggggcttc aggacatcgt gatcgggatt atcaagactg ttgctcaaca 
      661 gatacacggc actgagatag acatgaaggt gattcagcaa agaaatgaag aatgtgatca 
      721 cacccagttt ttaattgaag aaaaggaatc aaaagaagag gatttttatg aagatctgga 
      781 taggtttgaa gagaatggta cccaggaatc acgtatcagc ccttacacct tctgcaaagc 
      841 atttcctttt cacatcatat ttgaccggaa cctagtggtc actcagtgtg gcaatgccat 
      901 ctacagagtg ctcccccagc tccagcctgg gaactgcagc cttctgtctg tcttctctct 
      961 ggtccgccct cacatcgaca tcagtttcca tgggattctt tcacacatca atacagtctt 
     1021 tgtactgaga agcaaggaag ggttgctgga tgtggagaaa cttgagtgtg aggatgaact 
     1081 gactggagca gagattagct gcttacgtct caaaggccaa atgatctact taccagaagc 
     1141 agatagcatc ctcttcctct gttcaccaag tgtgatgaac ctggacgacc taacaagaag 
     1201 aggcctgtat ctgagtgaca tccctctcca cgatgctacc cgagacctgg ttcttttggg 
     1261 agaacagttc cgggaggagt acaaactgac acaagagctg gaaatcctca ccgacaggct 
     1321 gcagctcaca ctgagagcct tggaggatga gaagaaaaag acagacacat tgctgtattc 
     1381 tgtccttcct ccatctgttg ccaatgagct gagacacaag cgcccagtgc ctgccaaaag 
     1441 atacgacaat gtgaccatcc tcttcagcgg cattgtgggc ttcaatgctt tctgtagcaa 
     1501 gcatgcatct ggagaagggg ccatgaagat tgtcaatctc ctcaacgatc tctacacccg 
     1561 atttgacaca ctgactgatt cacgaaaaaa cccatttgtt tacaaggtgg aaacagttgg 
     1621 tgacaagtat atgacagtga gtggcttgcc agaaccttgt atccaccatg cacggtccat 
     1681 ttgccacctg gctttagaca tgatggaaat tgctggtcaa gttcaagtag atggtgaatc 
     1741 tgttcagata acaatcggga tccataccgg ggaggtggtg acaggtgtga ttggacagcg 
     1801 gatgcctcgg tattgtctct ttgggaatac cgtcaacctc acaagcagga cagaaaccac 
     1861 aggagaaaag ggaaagataa atgtttccga atatacatac aggtgtctca tgtctccaga 
     1921 aaactcggat ccactgttcc atttggagca cagaggccca gtgtctatga agggcaagaa 
     1981 ggaaccaatg caagtctggt tcctatccag gaaaaataca ggcacggagg aaacaaatga 
     2041 ggaggatgaa aactgagtgt caggttgtgg aatctagagc aagggcaatt cgaagcttag 
     2101 gcctggtaag cctatcccta accctctcct cggtctcgat tctacgcgta ccggtcatca 
     2161 tcaccatcac cattgacgtc tctagcttgg agtcgacaac ttgtttattg cagcttataa 
     2221 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 
     2281 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catctctcga ctctgctgaa 
     2341 gaggaggaaa ttctccttga agtttccctg gtgttcaaag taaaggagtt tgcaccagac 
     2401 gcacctctgt tcactggtcc ggcgtattaa aacacgatac attgttatta gtacatttat 
     2461 taagcgctag attctgtgcg ttgttgattt acagacaatt gttgtacgta ttttaataat 
     2521 tcattaaatt tataatcttt agggtggtat gttagagcga aaatcaaatg attttcagcg 
     2581 tctttatatc tgaatttaaa tattaaatcc tcaatagatt tgtaaaatag gtttcgatta 
     2641 gtttcaaaca agggttgttt ttccgaaccg atggctggac tatctaatgg attttcgctc 
     2701 aacgccacaa aacttgccaa atcttgtagc agcaatctag ctttgtcgat attcgtttgt 
     2761 gttttgtttt gtaataaagg ttcgacgtcg ttcaaaatat tatgcgcttt tgtatttctt 
     2821 tcatcactgt cgttagtgta caattgactc gacgtaaaca cgttaaataa agcctggaca 
     2881 tatttaacat cgggcgtgtt agctttatta ggccgattat cgtcgtcgtc ccaaccctcg 
     2941 tcgttagaag ttgcttccga agacgatttt gccatagcca cacgacgcct attaattgtg 
     3001 tcggctaaca cgtccgcgat caaatttgta gttgagcttt ttggaattat ttctgattgc 
     3061 gggcgttttt gggcgggttt caatctaact gtgcccgatt ttaattcaga caacacgtta 
     3121 gaaagcgatg gtgcaggcgg tggtaacatt tcagacggca aatctactaa tggcggcggt 
     3181 ggtggagctg atgataaatc taccatcggt ggaggcgcag gcggggctgg cggcggaggc 
     3241 ggaggcggag gtggtggcgg tgatgcagac ggcggtttag gctcaaatgt ctctttaggc 
     3301 aacacagtcg gcacctcaac tattgtactg gtttcgggcg atggtgcact ctcagtacaa 
     3361 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc 
     3421 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga 
     3481 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg 
     3541 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 
     3601 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 
     3661 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 
     3721 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 
     3781 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 
     3841 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 
     3901 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 
     3961 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 
     4021 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 
     4081 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 
     4141 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 
     4201 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 
     4261 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 
     4321 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 
     4381 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 
     4441 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 
     4501 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 
     4561 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 
     4621 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 
     4681 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 
     4741 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 
     4801 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 
     4861 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 
     4921 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 
     4981 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 
     5041 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 
     5101 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 
     5161 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 
     5221 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 
     5281 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 
     5341 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 
     5401 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 
     5461 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 
     5521 catcctgcac catcgtctgc tcatccatga cctgaccatg cagaggatga tgctcgtgac 
     5581 ggttaacgcc tcgaatcagc aacggcttgc cgttcagcag cagcagacca ttttcaatcc 
     5641 gcacctcgcg gaaaccgaca tcgcaggctt ctgcttcaat cagcgtgccg tcggcggtgt 
     5701 gcagttcaac caccgcacga tagagattcg ggatttcggc gctccacagt ttcgggtttt 
     5761 cgacgttcag acgtagtgtg acgcgatcgg cataaccacc acgctcatcg ataatttcac 
     5821 cgccgaaagg cgcggtgccg ctggcgacct gcgtttcacc ctgccataaa gaaactgtta 
     5881 cccgtaggta gtcacgcaac tcgccgcaca tctgaacttc agcctccagt acagcgcggc 
     5941 tgaaatcatc attaaagcga gtggcaacat ggaaatcgct gatttgtgta gtcggtttat 
     6001 gcagcaacga gacgtcacgg aaaatgccgc tcatccgcca catatcctga tcttccagat 
     6061 aactgccgtc actccagcgc agcaccatca ccgcgaggcg gttttctccg gcgcgtaaaa 
     6121 atgcgctcag gtcaaattca gacggcaaac gactgtcctg gccgtaaccg acccagcgcc 
     6181 cgttgcacca cagatgaaac gccgagttaa cgccatcaaa aataattcgc gtctggcctt 
     6241 cctgtagcca gctttcatca acattaaatg tgagcgagta acaacccgtc ggattctccg 
     6301 tgggaacaaa cggcggattg accgtaatgg gataggtcac gttggtgtag atgggcgcat 
     6361 cgtaaccgtg catctgccag tttgagggga cgacgacagt atcggcctca ggaagatcgc 
     6421 actccagcca gctttccggc accgcttctg gtgccggaaa ccaggcaaag cgccattcgc 
     6481 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc 
     6541 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc 
     6601 agtcacgacg ttgtaaaacg acgggatcta tcatttttag cagtgattct aattgcagct 
     6661 gctctttgat acaactaatt ttacgacgac gatgcgagct tttattcaac cgagcgtgca 
     6721 tgtttgcaat cgtgcaagcg ttatcaattt ttcattatcg tattgttgca catcaacagg 
     6781 ctggacacca cgttgaactc gccgcagttt tgcggcaagt tggacccgcc gcgcatccaa 
     6841 tgcaaacttt ccgacattct gttgcctacg aacgattgat tctttgtcca ttgatcgaag 
     6901 cgagtgcctt cgactttttc gtgtccagtg tggctt 
//
