LOCUS       pCAGGS-EhRhoA-DN        5468 bp    DNA     circular     24-OCT-2007
DEFINITION  LMBP 4657.
SOURCE      
  ORGANISM  
COMMENT     LMBP 4657
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 24-OCT-2007
            
COMMENT     VNTDATE|427722766|
COMMENT     VNTNAME|pCAGGS-EhRhoA-DN|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-EhRhoA-DN" 0 5468 
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COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 21 22 62 29 30 32 33 43) 
COMMENT        (CWordArray) (CStringList "BamHI" "Eco52I" "EcoRI" "NotI" "XmaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5468 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 3 670 0 1408 0 1745 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "Eco52I" "cggccg" 2 0 2 57 0 4753 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 4 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 644 0 0 "")) 
COMMENT       (CObList 
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COMMENT        #6=(CFSignal (CObList) "intron" 15 0 0 4493 5417 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4493..5417") 
COMMENT        #7=(CFSignal (CObList) "AG promoter" 29 0 0 4124 5465 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4124..5465") 
COMMENT        #8=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 3741 4118 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "3741..4118") 
COMMENT        #9=(CFSignal (CObList) "ampicillin" 4 0 1 2745 3605 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2745..3605)") 
COMMENT        #10=(CFSignal (CObList) "pMB1 ori" 33 0 1 1803 2204 0 (CStringList) 
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COMMENT        #11=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1614 1688 0 
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COMMENT        #13=(CFSignal (CObList) "SV40 ori" 33 0 0 1508 1583 0 (CStringList) 
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COMMENT        #14=(CFSignal (CObList) "lac promoter" 30 0 1 1284 1366 0 (CStringList) 
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COMMENT        #15=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 850 1206 0 
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COMMENT        #16=(CFSignal (CObList) "\337-globin polyA" 43 0 0 758 849 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "758..849") 
COMMENT        #17=(CFSignal (CObList) "hRhoA-DN" 4 0 0 64 642 0 (CStringList) 
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COMMENT                          "Created: 11/22/06 11:52" 1) 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
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FEATURES             Location/Qualifiers
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
                     /note="E-tag, N-terminal"
     intron          4493..5417
                     /vntifkey="15"
                     /label=intron
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter intron"
     promoter        4124..5465
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        3741..4118
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="human cytomegalovirus immediate early promoter (CMV-IE); enhancer only"
     CDS             complement(2745..3605)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      complement(1803..2204)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     terminator      complement(1614..1688)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     terminator      1614..1688
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     rep_origin      1508..1583
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     promoter        complement(1284..1366)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     misc_feature    850..1206
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit ß-globin gene"
     terminator      758..849
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     CDS             64..642
                     /vntifkey="4"
                     /label=hRhoA-DN
BASE COUNT     1178 a      1415 c      1539 g      1336 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gctgctgcca tccggaagaa actggtgatt gttggtgatg gagcctgtgg aaagaactgc 
      121 ttgctcatag tcttcagcaa ggaccagttc ccagaggtgt atgtgcccac agtgtttgag 
      181 aactatgtgg cagatatcga ggtggatgga aagcaggtag agttggcttt gtgggacaca 
      241 gctgggcagg aagattatga tcgcctgagg cccctctcct acccagatac cgatgttata 
      301 ctgatgtgtt tttccatcga cagccctgat agtttagaaa acatcccaga aaagtggacc 
      361 ccagaagtca agcatttctg tcccaacgtg cccatcatcc tggttgggaa taagaaggat 
      421 cttcggaatg atgagcacac aaggcgggag ctagccaaga tgaagcagga gccggtgaaa 
      481 cctgaagaag gcagagatat ggcaaacagg attggcgctt ttgggtacat ggagtgttca 
      541 gcaaagacca aagatggagt gagagaggtt tttgaaatgg ctacgagagc tgctctgcaa 
      601 gctagacgtg ggaagaaaaa atctggttgc cttgtcttgt gacccgggac attaaacgcg 
      661 tgggtaccgg atccggcaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc 
      721 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa 
      781 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat 
      841 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag 
      901 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca 
      961 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc 
     1021 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat 
     1081 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact 
     1141 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg 
     1201 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt 
     1261 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 
     1321 cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 
     1381 ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc 
     1441 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 
     1501 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 
     1561 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt 
     1621 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 
     1681 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 
     1741 tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 
     1801 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 
     1861 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga 
     1921 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 
     1981 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 
     2041 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 
     2101 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 
     2161 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 
     2221 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 
     2281 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 
     2341 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 
     2401 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 
     2461 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 
     2521 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 
     2581 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 
     2641 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 
     2701 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 
     2761 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 
     2821 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 
     2881 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 
     2941 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 
     3001 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 
     3061 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 
     3121 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 
     3181 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 
     3241 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 
     3301 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 
     3361 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 
     3421 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 
     3481 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 
     3541 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 
     3601 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 
     3661 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 
     3721 cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac 
     3781 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg 
     3841 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc 
     3901 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac 
     3961 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa 
     4021 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac 
     4081 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt 
     4141 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt 
     4201 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc 
     4261 ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag 
     4321 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa 
     4381 gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc 
     4441 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg 
     4501 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc 
     4561 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag 
     4621 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct 
     4681 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg 
     4741 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa 
     4801 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg 
     4861 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc 
     4921 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt 
     4981 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg 
     5041 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat 
     5101 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat 
     5161 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca 
     5221 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc 
     5281 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg 
     5341 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct 
     5401 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg 
     5461 gcaaagaa 
//
