LOCUS       pCAGGS-EhRac1-DN        5471 bp    DNA     circular     24-OCT-2007
DEFINITION  LMBP 4659.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4659
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 24-OCT-2007
COMMENT     VNTDATE|429793234|
COMMENT     VNTNAME|pCAGGS-EhRac1-DN|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-EhRac1-DN" 0 5471 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 21 22 62 29 30 32 33 43) 
COMMENT        (CWordArray) (CStringList "BamHI" "Eco52I" "EcoRI" "NotI" "XmaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 100 120 1 5471 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 3 673 0 1411 0 1748 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "Eco52I" "cggccg" 2 0 2 57 0 4756 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 4 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 647 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "E-tag" 22 0 0 16 54 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "16..54") 
COMMENT        #6=(CFSignal (CObList) "intron" 15 0 0 4496 5420 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4496..5420") 
COMMENT        #7=(CFSignal (CObList) "AG promoter" 29 0 0 4127 5468 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4127..5468") 
COMMENT        #8=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 3744 4121 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "3744..4121") 
COMMENT        #9=(CFSignal (CObList) "ampicillin" 4 0 1 2748 3608 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2748..3608)") 
COMMENT        #10=(CFSignal (CObList) "pMB1 ori" 33 0 1 1806 2207 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1806..2207)") 
COMMENT        #11=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1617 1691 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(1617..1691)") 
COMMENT        #12=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1617 1691 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1617..1691") 
COMMENT        #13=(CFSignal (CObList) "SV40 ori" 33 0 0 1511 1586 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1511..1586") 
COMMENT        #14=(CFSignal (CObList) "lac promoter" 30 0 1 1287 1369 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1287..1369)") 
COMMENT        #15=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 853 1209 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "853..1209") 
COMMENT        #16=(CFSignal (CObList) "\337-globin polyA" 43 0 0 761 852 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "761..852") 
COMMENT        #17=(CFSignal (CObList) "hRac1-DN" 4 0 0 64 639 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "64..639")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList 
COMMENT        #18=(CFSignal (CObList) "LMBP 4659" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #19=(CFSignal (CObList) "Last modified: 24-OCT-2007" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pCAGGS-EhRac1-DN'" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4659." 
COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 12/14/06 11:00" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 10/24/07 10:14" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5471 bp" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
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COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 14-DEC-2006" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                   "Annotations") 
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COMMENT                                        "Start: 64    End: 639 " 1) 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "human small GTP binding protein Rac1 cDNA; dominant-negative T17N mutant" 
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COMMENT                                   #53=(CLinePar 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
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COMMENT                                 "ampicillin"))) "CDS (2 total)") 
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COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid promoter intron" 
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COMMENT                          "Intron (1 total)") 
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COMMENT                                        "Start: 853   End: 1209" 1) 
COMMENT                                   #65=(CLinePar 
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COMMENT                                        "3' flanking region of the rabbit \337-globin gene" 
COMMENT                                        1) 
COMMENT                                   #66=(CLinePar 
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COMMENT                                 "3' FR of \337-globin"))) 
COMMENT                          "Misc. Feature (1 total)") 
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COMMENT                                   #73=(CLinePar 
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COMMENT                                        "   16..54" 1))) "E-tag"))) 
COMMENT                          "Misc. Marker (1 total)") 
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COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
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COMMENT                                        "Start: 3744  End: 4121" 1) 
COMMENT                                   #77=(CLinePar 
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COMMENT                                        "human cytomegalovirus immediate early promoter (CMV-IE); enhancer only" 
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COMMENT                                   #78=(CLinePar 
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COMMENT                                   #79=(CLinePar 
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COMMENT                                        "Start: 4127  End: 5468" 1) 
COMMENT                                   #82=(CLinePar 
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COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid promoter" 
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COMMENT                                   #84=(CLinePar 
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COMMENT                                        "   4127..5468" 1))) "AG promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                   #88=(CLinePar 
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COMMENT                                        "E. coli lac operon promoter" 1) 
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COMMENT                                        "   complement(1287..1369)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                   "Feature Map") 
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FEATURES             Location/Qualifiers
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
                     /note="E-tag, N-terminal"
     intron          4496..5420
                     /vntifkey="15"
                     /label=intron
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter intron"
     promoter        4127..5468
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        3744..4121
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="human cytomegalovirus immediate early promoter (CMV-IE); enhancer only"
     CDS             complement(2748..3608)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      complement(1806..2207)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     terminator      complement(1617..1691)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     terminator      1617..1691
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     rep_origin      1511..1586
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     promoter        complement(1287..1369)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     misc_feature    853..1209
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit ß-globin gene"
     terminator      761..852
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     CDS             64..639
                     /vntifkey="4"
                     /label=hRac1-DN
                     /note="human small GTP binding protein Rac1 cDNA; dominant-negative T17N mutant"
BASE COUNT     1176 a      1436 c      1514 g      1345 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gctcaggcca tcaagtgtgt ggtggtggga gacggagctg taggtaaaaa ttgcctactg 
      121 atcagttaca caaccaatgc atttcctgga gaatatatcc ctactgtctt tgacaattat 
      181 tctgccaatg ttatggtaga tggaaaaccg gtgaatctgg gcttatggga tacagctgga 
      241 caagaagatt atgacagatt acgcccccta tcctatccgc aaacagatgt gttcttaatt 
      301 tgcttttccc ttgtgagtcc tgcatcattt gaaaatgtcc gtgcaaagtg gtatcctgag 
      361 gtgcggcacc actgtcccaa cactcccatc atcctagtgg gaactaaact tgatcttagg 
      421 gatgataaag acacgatcga gaaactgaag gagaagaagc tgactcccat cacctatccg 
      481 cagggtctag ccatggctaa ggagattggt gctgtaaaat acctggagtg ctcggcgctc 
      541 acacagcgag gcctcaagac agtgtttgac gaagcgatcc gagcagtcct ctgcccgcct 
      601 cccgtgaaga agaggaagag aaaatgcctg ctgttgtgac tcgagcccgg gacattaaac 
      661 gcgtgggtac cggatccggc aattcactcc tcaggtgcag gctgcctatc agaaggtggt 
      721 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa 
      781 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt 
      841 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg 
      901 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc 
      961 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca 
     1021 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt 
     1081 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt 
     1141 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt 
     1201 atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg 
     1261 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 
     1321 aagcctgggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 
     1381 tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata 
     1441 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 
     1501 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 
     1561 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact 
     1621 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 
     1681 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 
     1741 atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 
     1801 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 
     1861 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag ggataacgca 
     1921 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 
     1981 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 
     2041 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 
     2101 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 
     2161 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 
     2221 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 
     2281 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 
     2341 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 
     2401 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag 
     2461 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 
     2521 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 
     2581 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 
     2641 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 
     2701 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 
     2761 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 
     2821 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 
     2881 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 
     2941 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 
     3001 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 
     3061 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 
     3121 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 
     3181 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 
     3241 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 
     3301 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 
     3361 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 
     3421 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 
     3481 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 
     3541 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 
     3601 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 
     3661 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 
     3721 ccccgaaaag tgccacctgg gtcgacattg attattgact agttattaat agtaatcaat 
     3781 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 
     3841 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 
     3901 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 
     3961 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 
     4021 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 
     4081 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac 
     4141 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat 
     4201 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcccc ccccaggcgg 
     4261 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca 
     4321 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa 
     4381 aaagcgaagc gcgcggcggg cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc 
     4441 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag 
     4501 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt 
     4561 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg 
     4621 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc 
     4681 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt 
     4741 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa 
     4801 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt 
     4861 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg 
     4921 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca 
     4981 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaaggggc 
     5041 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt 
     5101 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga 
     5161 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg 
     5221 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc 
     5281 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg 
     5341 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg 
     5401 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt 
     5461 ttggcaaaga a 
//
