ID pCAGGS-EhRac1-DP standard; circular DNA; ; 5471 BP. DE LMBP 4660. CC Accession number: LMBP 4660 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 24-OCT-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_marker 16..54 FT /vntifkey="22" FT /label=E-tag FT /note="E-tag, N-terminal" FT intron 4496..5420 FT /vntifkey="15" FT /label=intron FT /note="chicken ß-actin/rabbit ß-globin hybrid promoter intron" FT promoter 4127..5468 FT /vntifkey="29" FT /label=AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid promoter" FT promoter 3744..4121 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT /note="human cytomegalovirus immediate early promoter (CMV-IE); enhancer only" FT CDS complement(2748..3608) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" FT rep_origin complement(1806..2207) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT terminator complement(1617..1691) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT terminator 1617..1691 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT rep_origin 1511..1586 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT promoter complement(1287..1369) FT /vntifkey="30" FT /label=lac\promoter FT /note="E. coli lac operon promoter" FT misc_feature 853..1209 FT /vntifkey="21" FT /label=3'\FR\of\ß-globin FT /note="3' flanking region of the rabbit ß-globin gene" FT terminator 761..852 FT /vntifkey="43" FT /label=ß-globin\polyA FT /note="3' UTR of the rabbit ß-globin gene, incl. polyA" FT CDS 64..639 FT /vntifkey="4" FT /label=hRac1-DP FT /note="human small GTP binding protein Rac1 cDNA; constitutive-active G12V mutant" SQ Sequence 5471 BP; 1175 A; 1437 C; 1513 G; 1346 t; ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 60 gctcaggcca tcaagtgtgt ggtggtggga gacgtagctg taggtaaaac ttgcctactg 120 atcagttaca caaccaatgc atttcctgga gaatatatcc ctactgtctt tgacaattat 180 tctgccaatg ttatggtaga tggaaaaccg gtgaatctgg gcttatggga tacagctgga 240 caagaagatt atgacagatt acgcccccta tcctatccgc aaacagatgt gttcttaatt 300 tgcttttccc ttgtgagtcc tgcatcattt gaaaatgtcc gtgcaaagtg gtatcctgag 360 gtgcggcacc actgtcccaa cactcccatc atcctagtgg gaactaaact tgatcttagg 420 gatgataaag acacgatcga gaaactgaag gagaagaagc tgactcccat cacctatccg 480 cagggtctag ccatggctaa ggagattggt gctgtaaaat acctggagtg ctcggcgctc 540 acacagcgag gcctcaagac agtgtttgac gaagcgatcc gagcagtcct ctgcccgcct 600 cccgtgaaga agaggaagag aaaatgcctg ctgttgtgac tcgagcccgg gacattaaac 660 gcgtgggtac cggatccggc aattcactcc tcaggtgcag gctgcctatc agaaggtggt 720 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa 780 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt 840 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg 900 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc 960 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca 1020 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt 1080 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt 1140 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt 1200 atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg 1260 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 1320 aagcctgggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 1380 tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata 1440 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 1500 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 1560 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact 1620 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 1680 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 1740 atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 1800 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 1860 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag ggataacgca 1920 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 1980 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 2040 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 2100 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 2160 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 2220 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 2280 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 2340 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 2400 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag 2460 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 2520 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 2580 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 2640 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 2700 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 2760 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 2820 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 2880 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 2940 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 3000 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 3060 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 3120 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 3180 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 3240 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 3300 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 3360 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 3420 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 3480 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 3540 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 3600 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 3660 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 3720 ccccgaaaag tgccacctgg gtcgacattg attattgact agttattaat agtaatcaat 3780 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 3840 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 3900 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 3960 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 4020 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 4080 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac 4140 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat 4200 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcccc ccccaggcgg 4260 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca 4320 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa 4380 aaagcgaagc gcgcggcggg cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc 4440 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag 4500 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt 4560 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg 4620 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc 4680 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt 4740 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa 4800 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt 4860 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg 4920 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca 4980 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaaggggc 5040 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt 5100 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga 5160 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg 5220 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc 5280 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg 5340 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg 5400 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt 5460 ttggcaaaga a 5471 //