LOCUS       pCAGGS-EhCdc42-DN        5462 bp    DNA     circular      9-OCT-2008
DEFINITION  LMBP 4661.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4661
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 9-OCT-2008
            
            
            
            
COMMENT     VNTDATE|430139488|
COMMENT     VNTNAME|pCAGGS-EhCdc42-DN|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-EhCdc42-DN" 0 5462 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 21 22 62 29 30 32 33 43) 
COMMENT        (CWordArray) (CStringList "XhoI" "Eco52I" "BamHI" "NcoI" "NotI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 4947 4946 1 5462 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "Eco52I" "cggccg" 2 0 2 57 0 4747 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 3 664 0 1402 0 1739 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 12 0 1495 0 4111 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "E-tag" 22 0 0 16 54 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "16..54") 
COMMENT        #6=(CFSignal (CObList) "intron" 15 0 0 4487 5411 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4487..5411") 
COMMENT        #7=(CFSignal (CObList) "AG promoter" 29 0 0 4118 5459 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4118..5459") 
COMMENT        #8=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 3735 4112 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "3735..4112") 
COMMENT        #9=(CFSignal (CObList) "ampicillin resistance" 4 0 1 2739 3599 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(2739..3599)") 
COMMENT        #10=(CFSignal (CObList) "pMB1 ori" 33 0 1 1797 2198 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1797..2198)") 
COMMENT        #11=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1608 1682 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(1608..1682)") 
COMMENT        #12=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1608 1682 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1608..1682") 
COMMENT        #13=(CFSignal (CObList) "SV40 ori" 33 0 0 1502 1577 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1502..1577") 
COMMENT        #14=(CFSignal (CObList) "lac promoter" 30 0 1 1278 1360 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1278..1360)") 
COMMENT        #15=(CFSignal (CObList) "human CDC42-DN" 4 0 0 64 636 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "64..636") 
COMMENT        #16=(CFSignal (CObList) "3' UTR of rabbit \337-globin, incl. polyA" 43 0 
COMMENT             0 752 843 0 (CStringList) (CStringList) 1 1 1 1 "752..843") 
COMMENT        #17=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 
COMMENT             0 844 1200 0 (CStringList) (CStringList) 1 1 1 1 "844..1200")) 
COMMENT       (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #18=(CFSignal (CObList) "LMBP 4661" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #19=(CFSignal (CObList) "Last modified: 9-OCT-2008" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #20=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #21=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) 
COMMENT                    "pCAGGS-EhCdc42-DN" 2) 5 "" 0 4) 
COMMENT              #22=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pCAGGS-EhCdc42-DN'" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4661." 
COMMENT                          1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 12/18/06 11:11" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 10/09/08 01:01" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5462 bp" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #31=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 9-OCT-2008" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #34=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "BCCM/GeneCorner Plasmid Collection" 1))) "Original Author") 
COMMENT              #36=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #37=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #38=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #39=(CLinePar (CParagraph 0 (15 #18# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 4661") 
COMMENT                     #40=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #41=(CLinePar (CParagraph 0 (15 #19# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 9-OCT-2008"))) 
COMMENT                   "Annotations") 
COMMENT              #42=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #43=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #44=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 64 (3 #15# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #45=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 64    End: 636 " 1) 
COMMENT                                   #46=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   64..636" 1))) "human CDC42-DN") 
COMMENT                            #48=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2739 (3 #9# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2739  End: 3599 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2739..3599)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (2 total)") 
COMMENT                     #52=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 15 (7 15 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #53=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4487 (3 #6# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4487  End: 5411" 1) 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4487..5411" 1))) "intron"))) 
COMMENT                          "Intron (1 total)") 
COMMENT                     #57=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 2 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #58=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 844 (3 #17# 0) 1 2 2 1 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 844   End: 1200" 1) 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   844..1200" 1))) 
COMMENT                                 "3' flanking region of rabbit \337-globin"))) 
COMMENT                          "Misc. Feature (1 total)") 
COMMENT                     #62=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #63=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 16 (3 #5# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 16    End: 54  " 1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   16..54" 1))) "E-tag"))) 
COMMENT                          "Misc. Marker (1 total)") 
COMMENT                     #67=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #68=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3735 (3 #8# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3735  End: 4112" 1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3735..4112" 1))) "hCMV-IE enhancer") 
COMMENT                            #72=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4118 (3 #7# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4118  End: 5459" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4118..5459" 1))) "AG promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #76=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #77=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1278 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1278  End: 1360 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1278..1360)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
COMMENT                     #81=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #82=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1502 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1502  End: 1577" 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1502..1577" 1))) "SV40 ori") 
COMMENT                            #86=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1797 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1797  End: 2198 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1797..2198)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (2 total)") 
COMMENT                     #90=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #91=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 752 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 752   End: 843 " 1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   752..843" 1))) 
COMMENT                                 "3' UTR of rabbit \337-globin, incl. polyA") 
COMMENT                            #95=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1608 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1608  End: 1682 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1608..1682)" 1))) 
COMMENT                                 "SV40 polyA late") 
COMMENT                            #99=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1608 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Start: 1608  End: 1682" 1) 
COMMENT                                   #101=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Original Location Description:" 1) 
COMMENT                                   #102=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   1608..1682" 1))) "SV40 polyA early"))) 
COMMENT                          "Terminator (3 total)"))) "Feature Map") 
COMMENT              #103=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #104=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 27334920 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #105=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #106=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 664 " 1) 
COMMENT                                      #107=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1402 " 1) 
COMMENT                                      #108=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 1739 " 1))))) "BamHI: 3 sites") 
COMMENT                            1 5 "GGATCC" "CCTAGG") 
COMMENT                      #109=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 27285848 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #110=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #111=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 57 " 1) 
COMMENT                                      #112=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 4747 " 1))))) 
COMMENT                             "Eco52I: 2 sites") 1 5 "CGGCCG" "GCCGGC") 
COMMENT                      #113=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 27868328 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #114=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #115=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 12 " 1) 
COMMENT                                      #116=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1495 " 1) 
COMMENT                                      #117=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 4111 " 1))))) "NcoI: 3 sites") 
COMMENT                            1 5 "CCATGG" "GGTACC") 
COMMENT                      #118=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 27196488 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #119=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #120=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 57 " 1))))) "NotI: 1 site") 2 
COMMENT                            6 "GCGGCCGC" "CGCCGGCG") 
COMMENT                      #121=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 27286784 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "XhoI: 0 sites") 1 5 "CTCGAG" 
COMMENT                            "GAGCTC"))) "Restriction/Methylation Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #122=(CWidgetStyle "~Scrap" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #123=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #124=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #125=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #126=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #127=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.64 0 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #128=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #129=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #130=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #131=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #132=(CWidgetStyle "Molecule Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #133=(CWidgetStyle "Molecule Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 "@N" 0) 
COMMENT          #134=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #135=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 255 0) 0 
COMMENT                0 0) 
COMMENT          #136=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #137=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 0 0 0) 
COMMENT          #138=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) 
COMMENT          #139=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH 0 12632256 0) 
COMMENT                0 0 0) #140=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #141=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #142=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #143=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #144=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #145=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #146=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #147=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #148=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0) 
COMMENT          #149=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #150=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #151=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #152=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #153=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #154=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #155=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #156=(CWidgetStyle "Label 0" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711935 1 5 "@N (@S)" 0) 
COMMENT          #157=(CWidgetStyle "Label 1" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #158=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 32768 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #159=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 16711680 
COMMENT                1 5 "@N (@S)" 0) 
COMMENT          #160=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #161=(CWidgetStyle "Label 5" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #162=(CWidgetStyle "Intron" 1 (LOGPEN 0 0 16711680) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #163=(CWidgetStyle "Label 6" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #164=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 3342336) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #165=(CWidgetStyle "Label 7" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #166=(CWidgetStyle "Label 8" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #167=(CWidgetStyle "Label 9" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #168=(CWidgetStyle "Label 10" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #169=(CWidgetStyle "Label 11" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #170=(CWidgetStyle "3'UTR Label" 1 (LOGPEN 0 0 13107) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #171=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #172=(CWidgetStyle "Enhancer Label" 1 (LOGPEN 0 0 13107) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #173=(CWidgetStyle "Intron Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #174=(CWidgetStyle "Misc_Feature Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #175=(CWidgetStyle "Marker Label" 1 (LOGPEN 0 0 0) 3 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #176=(CWidgetStyle "Promoter_E Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #177=(CWidgetStyle "Promoter_P Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #178=(CWidgetStyle "Rep_Origin Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #179=(CWidgetStyle "Terminator Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #180=(CWidgetStyle "Label 12" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #181=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.888889 0 1 
COMMENT                65535 "@N" 0) 
COMMENT          #182=(CWidgetStyle "oriT Label" 1 (LOGPEN 0 0 13107) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #183=(CWidgetStyle "Uniq RSite" 1 (LOGPEN 0 0 16750899) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #184=(CWidgetStyle "Label 13" 1 (LOGPEN 0 0 16763904) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #185=(CWidgetStyle "Label 14" 1 (LOGPEN 0 0 39168) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #186=(CWidgetStyle "Label 15" 1 (LOGPEN 0 0 16750848) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #187=(CWidgetStyle "Marker" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #188=(CWidgetStyle "Shape 6" 1 (LOGPEN 0 0 0) 3 1 (LOGBRUSH 0 128 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #189=(CWidgetStyle "Shape 7" 1 (LOGPEN 0 0 16711680) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #190=(CWidgetStyle "Shape 8" 1 (LOGPEN 0 0 3381504) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #191=(CWidgetStyle "Label 16" 1 (LOGPEN 0 0 16724787) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #192=(CWidgetStyle "Shape 9" 1 (LOGPEN 0 0 16737792) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #193=(CWidgetStyle "Label 17" 1 (LOGPEN 0 0 16737792) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #194=(CWidgetStyle "Label 18" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #195=(CWidgetStyle "Promoter_E" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #196=(CWidgetStyle "Shape 10" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #197=(CWidgetStyle "Label 19" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #198=(CWidgetStyle "Shape 11" 1 (LOGPEN 0 0 153) 3 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #199=(CWidgetStyle "Shape 12" 1 (LOGPEN 0 0 10040064) 4 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #200=(CWidgetStyle "Shape 13" 1 (LOGPEN 0 0 10040064) 6 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #201=(CWidgetStyle "Label 20" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #202=(CWidgetStyle "Label 21" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #203=(CWidgetStyle "RBS Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #204=(CWidgetStyle "Label 22" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 32768 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #205=(CWidgetStyle "Label 23" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #206=(CWidgetStyle "Shape 14" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #207=(CWidgetStyle "Attenuator" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #208=(CWidgetStyle "Shape 15" 1 (LOGPEN 0 0 6684774) 4 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #209=(CWidgetStyle "Promoter_P" 1 (LOGPEN 0 0 6684774) 8 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #210=(CWidgetStyle "Shape 16" 1 (LOGPEN 0 0 6684774) 7 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #211=(CWidgetStyle "Shape 17" 1 (LOGPEN 0 0 0) 7 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #212=(CWidgetStyle "Shape 18" 1 (LOGPEN 0 0 0) 2 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #213=(CWidgetStyle "Label 24" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #214=(CWidgetStyle "Label 25" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #215=(CWidgetStyle "Label 26" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #216=(CWidgetStyle "Attenuator Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #217=(CWidgetStyle "Shape 19" 1 (LOGPEN 0 0 6723891) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #218=(CWidgetStyle "Shape 20" 1 (LOGPEN 0 0 6723891) 1 1 
COMMENT                (LOGBRUSH 0 6723891 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #219=(CWidgetStyle "Shape 21" 1 (LOGPEN 0 0 3368448) 1 1 
COMMENT                (LOGBRUSH 0 6723891 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #220=(CWidgetStyle "Shape 22" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 6723891 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #221=(CWidgetStyle "Shape 23" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 52479 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #222=(CWidgetStyle "Shape 24" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 3381759 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #223=(CWidgetStyle "Shape 25" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 10066329 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #224=(CWidgetStyle "Shape 26" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 52275 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #225=(CWidgetStyle "Shape 27" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16763904 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #226=(CWidgetStyle "Shape 28" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 13395456 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #227=(CWidgetStyle "Shape 29" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 13408563 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #228=(CWidgetStyle "Shape 30" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16750848 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)) 
COMMENT         0.164644 1.74233 0.164644 2.53336 
COMMENT         (15 (CShapeMapEntry 0 "Shape 5" 1 "Label 20") 9 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Position Label") 4 
COMMENT          (CShapeMapEntry 0 "Shape 3" 1 "Label 20") 0 
COMMENT          (CShapeMapEntry 8 "Shape 30" 1 "Attenuator Label") 30 
COMMENT          (CShapeMapEntry 0 "Shape 18" 1 "Label 20") 29 
COMMENT          (CShapeMapEntry 0 "Promoter_E" 1 "Label 20") 26 
COMMENT          (CShapeMapEntry 0 "Line 0" 1 "Position Label") 25 
COMMENT          (CShapeMapEntry 0 "Line 4" 1 "Position Label") 22 
COMMENT          (CShapeMapEntry 1 "Shape 13" 1 "Label 20") 21 
COMMENT          (CShapeMapEntry 5 "Line 2" 1 "Label 20") 43 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 20") 33 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Label 20") 32 
COMMENT          (CShapeMapEntry 0 "Line 3" 1 "Label 20") 59 
COMMENT          (CShapeMapEntry 0 "Shape 10" 1 "Signal Label") 52 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Position Label") 70 
COMMENT          (CShapeMapEntry 0 "Shape 9" 1 "Label 16") 68 
COMMENT          (CShapeMapEntry 0 "Shape 8" 1 "Label 23") 86 
COMMENT          (CShapeMapEntry 8 "Shape 1" 1 "Label 26") 85 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Label 10")) 44.1479 44.1479 39 39 0.1 
COMMENT         -11477) 1 1 0 1 1 
COMMENT        (mapper: 21.5216 -16.0626 44.1479 44.1479 0.01 10 -1 5462 5462 1 0 0) 
COMMENT        #229=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 1701670255 100) 
COMMENT              (CObjectList 
COMMENT               #230=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -1041 100) 
COMMENT                     (CObjectList 
COMMENT                      #231=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #139# 21876804 0) 
COMMENT                             (LOGPEN 0 0 0) 2 (LOGBRUSH 0 12632256 0) 1 6.27533 
COMMENT                             6.27333 1 0.0214037) 0.114851) 
COMMENT                      #232=(CLabel 
COMMENT                            (CWidget 1001 (0 0) 1 2 0 0 #133# 1096045635 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 69 26 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.91 0 "pCAGGS-EhCdc42-DN" "@N" 1 0 0.871165 
COMMENT                            0 -10 13.624 1.56571 Nil) 
COMMENT                      #233=(CLabel (CWidget 1002 (0 0) 1 2 0 0 #132# 0 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 80 30 0 0 400 0 0 0 0 3 2 1 49 
COMMENT                             "Courier New") 1.74233 1.05 0 "5462 bp" "@L bp" 16 
COMMENT                            0 -0.871165 0 -10.8 5.00092 1.75266 Nil)) 
COMMENT                     (CObjectList)) 
COMMENT               #234=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -964 100) 
COMMENT                     (CObjectList 
COMMENT                      #235=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -349 100) 
COMMENT                            (CObjectList 
COMMENT                             #236=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #9# 0) 1 2 0 0 #135# 
COMMENT                                     2081903016 100) (LOGPEN 0 0 0) 1 
COMMENT                                    (LOGBRUSH 0 255 0) 1 2.13968 3.12855 1 
COMMENT                                    0.082322) 0.8 1.8 1) 
COMMENT                             #237=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #201# 2081903016 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 19 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "ampicillin resistance" "@N" 1 -10.8903 
COMMENT                                   -10.454 0 -10 8.41277 1.14507 #236#) 
COMMENT                             #238=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #15# 0) 1 2 0 0 #135# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    5.54273 6.20083 1 0.082322) 0.8 1.8 0) 
COMMENT                             #239=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #201# 27340172 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 19 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "human CDC42-DN" 
COMMENT                                   "@N" 1 14.2217 6.96932 0 -10 7.80518 1.14507 
COMMENT                                   #238#)) (CObjectList)) 
COMMENT                      #240=(CGroupWidget 
COMMENT                            (CWidget 15 (7 15 0) 1 2 0 0 Nil -734 100) 
COMMENT                            (CObjectList 
COMMENT                             #241=(CLine 
COMMENT                                   (CWidget 0 (3 #6# 0) 1 2 0 0 #164# 1918985504 
COMMENT                                    100) (LOGPEN 0 0 3342336) 1 0.835356 
COMMENT                                   0.0585742 1.12095) 
COMMENT                             #242=(CLabel (CWidget 0 (0 0) 1 2 0 0 #201# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 19 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "intron" "@N" 1 
COMMENT                                   -8.581 10.454 0 -10 2.33688 1.14507 #241#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #243=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil -426 100) 
COMMENT                            (CObjectList 
COMMENT                             #244=(CLine 
COMMENT                                   (CWidget 0 (3 #17# 0) 1 2 0 0 #143# 0 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.835356 4.89497 
COMMENT                                   5.30499) 
COMMENT                             #245=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #201# 21506228 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 19 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' flanking region of rabbit \337-globin" 
COMMENT                                   "@N" 1 19.409 1.74233 0 -10 14.3017 1.14507 
COMMENT                                   #244#)) (CObjectList)) 
COMMENT                      #246=(CGroupWidget 
COMMENT                            (CWidget 22 (7 22 0) 1 2 0 0 Nil -1272 100) 
COMMENT                            (CObjectList 
COMMENT                             #247=(CScratch 
COMMENT                                   (CWidget 0 (3 #5# 0) 1 2 0 0 #200# 0 100) 
COMMENT                                   (LOGPEN 0 4 10040064) 6 1 6.23414 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #248=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #201# 36935480 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 19 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "E-tag" "@N" 1 
COMMENT                                   2.58146 12.1963 0 -10 2.2434 1.14507 #247#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #249=(CGroupWidget 
COMMENT                            (CWidget 29 (7 29 0) 1 2 0 0 Nil -811 100) 
COMMENT                            (CObjectList 
COMMENT                             #250=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #7# 0) 1 2 0 0 #195# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 
COMMENT                                    1.01039 0.00344554 1.54475 1 0.082322) 0.8 
COMMENT                                   1.8 0) 
COMMENT                             #251=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #201# 14352944 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 19 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "AG promoter" 
COMMENT                                   "@N" 1 -11.6701 8.71165 0 -10 5.35145 1.14507 
COMMENT                                   #250#) 
COMMENT                             #252=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #8# 0) 1 2 0 0 #195# 
COMMENT                                     2081903016 100) (LOGPEN 0 0 0) 1 
COMMENT                                    (LOGBRUSH 0 8388736 0) 1 1.55049 1.98463 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #253=(CLabel (CWidget 0 (0 0) 1 2 0 0 #201# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 19 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "hCMV-IE enhancer" "@N" 1 -16.0376 -1.74233 0 
COMMENT                                   -10 7.94539 1.14507 #252#)) (CObjectList)) 
COMMENT                      #254=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil -272 100) 
COMMENT                            (CObjectList 
COMMENT                             #255=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #14# 0) 1 2 0 0 #212# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 2 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    4.71121 4.80653 1 0.082322) 0.8 1.8 1) 
COMMENT                             #256=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #201# 21562036 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 19 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "lac promoter" 
COMMENT                                   "@N" 1 14.7199 0 0 -10 5.18787 1.14507 #255#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #257=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil -195 100) 
COMMENT                            (CObjectList 
COMMENT                             #258=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #10# 0) 1 2 0 0 #134# 
COMMENT                                     2081903016 100) (LOGPEN 0 0 0) 1 
COMMENT                                    (LOGBRUSH 2 8388608 5) 1 3.74875 4.21045 1 
COMMENT                                    0.082322) 0.8 1.8 1) 
COMMENT                             #259=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #201# 160593936 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 19 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" 
COMMENT                                   1 11.6888 -12.1963 0 -10 3.73901 1.14507 
COMMENT                                   #258#) 
COMMENT                             #260=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #13# 0) 1 2 0 0 #134# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    4.46198 4.54927 1 0.082322) 0.8 1.8 0) 
COMMENT                             #261=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #201# 27658012 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 19 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "SV40 ori" "@N" 
COMMENT                                   1 14.6747 -5.15904 0 -10 3.59879 1.14507 
COMMENT                                   #260#)) (CObjectList)) 
COMMENT                      #262=(CGroupWidget 
COMMENT                            (CWidget 43 (7 43 0) 1 2 0 0 Nil -657 100) 
COMMENT                            (CObjectList 
COMMENT                             #263=(CLine 
COMMENT                                   (CWidget 0 (3 #11# 0) 1 2 0 0 #143# 
COMMENT                                    1129600084 100) (LOGPEN 0 6 16711680) 8 
COMMENT                                   0.835356 4.34138 4.42752) 
COMMENT                             #264=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #201# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 19 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 polyA late" "@N" 1 15.1669 -6.96932 0 
COMMENT                                   -10 6.68348 1.14507 #263#) 
COMMENT                             #265=(CLine 
COMMENT                                   (CWidget 0 (3 #12# 0) 1 2 0 0 #143# 
COMMENT                                    1952541728 100) (LOGPEN 0 6 16711680) 8 
COMMENT                                   0.670712 4.34138 4.42752) 
COMMENT                             #266=(CLabel (CWidget 0 (0 0) 1 2 0 0 #201# 1 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 19 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 polyA early" "@N" 1 15.5853 -8.71165 0 
COMMENT                                   -10 7.22096 1.14507 #265#) 
COMMENT                             #267=(CLine 
COMMENT                                   (CWidget 0 (3 #16# 0) 1 2 0 0 #143# 
COMMENT                                    1869422700 100) (LOGPEN 0 6 16711680) 8 
COMMENT                                   0.835356 5.30499 5.41065) 
COMMENT                             #268=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #201# 21603572 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 19 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' UTR of rabbit \337-globin, incl. polyA" 
COMMENT                                   "@N" 1 19.4216 3.48466 0 -10 14.956 1.14507 
COMMENT                                   #267#)) (CObjectList))) (CObjectList)) 
COMMENT               #269=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -1195 100) 
COMMENT                     (CObjectList 
COMMENT                      #270=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #4# 0) 1 2 0 0 Nil -1222 100) 
COMMENT                            (CObjectList 
COMMENT                             #271=(CScratch 
COMMENT                                   (CWidget 1 (1 #4# 1) 1 2 0 0 #192# 0 100) 
COMMENT                                   (LOGPEN 0 0 16737792) 1 1 6.20887 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #272=(CLabel (CWidget 0 (0 0) 1 2 0 0 #191# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Not}I (57)" "@N (@S)" 5 8.40749 10.454 
COMMENT                                   0 -10 3.43521 1.0516 #271#)) (CObjectList)) 
COMMENT                      #273=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #1# 0) 1 2 0 0 Nil -786 100) 
COMMENT                            (CObjectList 
COMMENT                             #274=(CScratch 
COMMENT                                   (CWidget 1 (1 #1# 1) 1 2 0 0 #190# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 6.20887 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #275=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #205# 14352952 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "{\\i Eco}52I (57)" "@N (@S)" 5 11.1846 
COMMENT                                   8.71165 0 -10 4.39333 1.0516 #274#) 
COMMENT                             #276=(CScratch 
COMMENT                                   (CWidget 2 (1 #1# 2) 1 2 0 0 #190# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 0.822336 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #277=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #205# 1668238368 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "{\\i Eco}52I (4747)" "@N (@S)" 5 -13.0543 
COMMENT                                   6.96932 0 -10 5.30472 1.0516 #276#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #278=(CGroupWidget 
COMMENT                            (CWidget 3 (10 #2# 0) 1 2 0 0 Nil -704 100) 
COMMENT                            (CObjectList 
COMMENT                             #279=(CScratch 
COMMENT                                   (CWidget 1 (1 #2# 1) 1 2 0 0 #190# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 5.51172 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #280=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #205# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "{\\i Bam}HI (664)" "@N (@S)" 5 13.701 
COMMENT                                   5.22699 0 -10 4.86071 1.0516 #279#) 
COMMENT                             #281=(CScratch 
COMMENT                                   (CWidget 2 (1 #2# 2) 1 2 0 0 #190# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 4.66412 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #282=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #205# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "{\\i Bam}HI (1402)" "@N (@S)" 5 14.9489 
COMMENT                                   -1.74233 0 -10 5.30472 1.0516 #281#) 
COMMENT                             #283=(CScratch 
COMMENT                                   (CWidget 3 (1 #2# 3) 1 2 0 0 #190# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 4.27707 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #284=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #205# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "{\\i Bam}HI (1739)" "@N (@S)" 5 14.4607 
COMMENT                                   -10.454 0 -10 5.30472 1.0516 #283#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #285=(CGroupWidget 
COMMENT                            (CWidget 3 (10 #3# 0) 1 2 0 0 Nil -349 100) 
COMMENT                            (CObjectList 
COMMENT                             #286=(CScratch 
COMMENT                                   (CWidget 1 (1 #3# 1) 1 2 0 0 #190# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 6.26055 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #287=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #205# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "{\\i Nco}I (12)" "@N (@S)" 5 3.17379 13.9386 
COMMENT                                   0 -10 3.55206 1.0516 #286#) 
COMMENT                             #288=(CScratch 
COMMENT                                   (CWidget 2 (1 #3# 2) 1 2 0 0 #190# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 4.5573 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #289=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #205# 27683228 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "{\\i Nco}I (1495)" "@N (@S)" 5 14.4297 
COMMENT                                   -3.48466 0 -10 4.46344 1.0516 #288#) 
COMMENT                             #290=(CScratch 
COMMENT                                   (CWidget 3 (1 #3# 3) 1 2 0 0 #190# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 1.55279 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #291=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #205# 1094796097 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "{\\i Nco}I (4111)" "@N (@S)" 5 -14.476 0 0 
COMMENT                                   -10 4.46344 1.0516 #290#)) (CObjectList))) 
COMMENT                     (CObjectList)) 
COMMENT               #292=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -173 100) 
COMMENT                     (CObjectList 
COMMENT                      #293=(CLabel (CWidget 0 (15 #18# 0) 1 2 0 0 #123# 707 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 69 26 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                             "Times New Roman") 1.74233 0.91 0 "LMBP 4661" 
COMMENT                            "LMBP 4661" 65535 0.226511 -2.53693 0 -10 7.03401 
COMMENT                            1.54234 Nil) 
COMMENT                      #294=(CLabel 
COMMENT                            (CWidget 0 (15 #19# 0) 1 2 0 0 #123# 1096045635 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 46 17 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.611111 0 "Last modified: 9-OCT-2008" 
COMMENT                            "Last modified: 9-OCT-2008" 65535 0.135907 -4.25841 
COMMENT                            0 -10 10.6562 1.0516 Nil)) (CObjectList)) 
COMMENT               #295=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -928 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 589826 
COMMENT       (CStringList) 524290 21103184 (CObList)))
FEATURES             Location/Qualifiers
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
                     /note="E-tag, N-terminal"
     intron          4487..5411
                     /vntifkey="15"
                     /label=intron
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter intron"
     promoter        4118..5459
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        3735..4112
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="human cytomegalovirus immediate early promoter (CMV-IE); enhancer only"
     CDS             complement(2739..3599)
                     /vntifkey="4"
                     /label=ampicillin\resistance
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      complement(1797..2198)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     terminator      complement(1608..1682)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     terminator      1608..1682
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     rep_origin      1502..1577
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     promoter        complement(1278..1360)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     misc_feature    844..1200
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     terminator      752..843
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     CDS             64..636
                     /vntifkey="4"
                     /label=human\CDC42-DN
BASE COUNT     1182 a      1422 c      1499 g      1359 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gctcagacaa ttaagtgtgt tgttgtgggc gatggtgctg ttggtaaaaa ctgtctcctg 
      121 atatcctaca caacaaacaa atttccatcg gaatatgtac cgactgtttt tgacaactat 
      181 gcagtcacag ttatgattgg tggagaacca tatactcttg gactttttga tactgcaggg 
      241 caagaggatt atgacagatt acgaccgctg agttatccac aaacagatgt atttctagtc 
      301 tgtttttcag tggtctctcc atcttcattt gaaaacgtga aagaaaagtg ggtgcctgag 
      361 ataactcacc actgtccaaa gactcctttc ttgcttgttg ggactcaaat tgatctcaga 
      421 gatgacccct ctactattga gaaacttgcc aagaacaaac agaagcctat cactccagag 
      481 actgctgaaa agctggcccg tgacctgaag gctgtcaagt atgtggagtg ttctgcactt 
      541 acacagaaag gcctaaagaa tgtatttgac gaagcaatat tggctgccct ggagcctcca 
      601 gaaccgaaga agagccgcag gtgtgtggtg ctatgacccg ggacattaaa cgcgtgggta 
      661 ccggatccgg caattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt 
      721 ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg 
      781 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat 
      841 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa 
      901 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct 
      961 ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc 
     1021 tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg 
     1081 ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag 
     1141 atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc 
     1201 cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 
     1261 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 
     1321 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 
     1381 gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc 
     1441 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 
     1501 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag 
     1561 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg 
     1621 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 
     1681 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga 
     1741 tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 
     1801 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 
     1861 agctcactca aaggcggtaa tacggttatc cacagaatca gggataacgc aggaaagaac 
     1921 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 
     1981 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 
     2041 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 
     2101 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 
     2161 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 
     2221 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 
     2281 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 
     2341 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 
     2401 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 
     2461 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 
     2521 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 
     2581 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 
     2641 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 
     2701 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 
     2761 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 
     2821 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 
     2881 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 
     2941 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 
     3001 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 
     3061 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 
     3121 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 
     3181 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 
     3241 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 
     3301 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 
     3361 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 
     3421 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 
     3481 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 
     3541 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 
     3601 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 
     3661 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 
     3721 gtgccacctg ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc 
     3781 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc 
     3841 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt 
     3901 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca 
     3961 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg 
     4021 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca 
     4081 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt 
     4141 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt 
     4201 attttgtgca gcgatggggg cggggggggg gggggggccc cccccaggcg gggcggggcg 
     4261 gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc 
     4321 gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag 
     4381 cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc 
     4441 ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga 
     4501 cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct 
     4561 gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc 
     4621 ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg 
     4681 cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg 
     4741 gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg 
     4801 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca 
     4861 accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct 
     4921 ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt 
     4981 gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc 
     5041 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat 
     5101 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga 
     5161 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg 
     5221 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc 
     5281 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg 
     5341 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct 
     5401 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag 
     5461 aa 
//
