LOCUS       pCAGGS-EYopE            5546 bp    DNA     circular     18-SEP-2007
DEFINITION  LMBP 4664.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4664
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 18-SEP-2007
COMMENT     VNTDATE|445018582|
COMMENT     VNTNAME|pCAGGS-EYopE|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-EYopE" 0 5546 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "EcoRI" "NotI" "XmaI" "MfeI" "BamHI" "NcoI" "PstI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5546 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 4 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 722 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "MfeI" "caattg" 2 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 3 748 0 1486 0 1823 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 12 0 1579 0 4195 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 450 0 1297 0 0 "")) 
COMMENT       (CObList 
COMMENT        #7=(CFSignal (CObList) "E-tag" 22 0 0 16 54 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "16..54") 
COMMENT        #8=(CFSignal (CObList) "pMB1 ori" 33 0 1 1881 2282 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1881..2282)") 
COMMENT        #9=(CFSignal (CObList) "ampicillin" 4 0 1 2823 3683 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2823..3683)") 
COMMENT        #10=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 3819 4196 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3819..4196") 
COMMENT        #11=(CFSignal (CObList) "intron AG promoter" 15 0 0 4571 5495 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4571..5495") 
COMMENT        #12=(CFSignal (CObList) "AG promoter" 29 0 0 4202 5543 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4202..5543") 
COMMENT        #13=(CFSignal (CObList) "YopE" 4 0 0 64 720 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "64..720") 
COMMENT        #14=(CFSignal (CObList) "\337-globin polyA" 43 0 0 836 927 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "836..927") 
COMMENT        #15=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 928 1284 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "928..1284") 
COMMENT        #16=(CFSignal (CObList) "SV40 ori" 33 0 0 1586 1661 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1586..1661") 
COMMENT        #17=(CFSignal (CObList) "lac promoter" 30 0 1 1362 1444 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1362..1483)") 
COMMENT        #18=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1692 1766 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1691..1766") 
COMMENT        #19=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1692 1766 0 
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COMMENT        #20=(CFSignal (CObList) "LMBP 4664" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #21=(CFSignal (CObList) "Last modified: 18-SEP-2007" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pCAGGS-EYopE'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Created: 04/30/07 04:16" 1) 
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COMMENT                          "Last Modified: 09/18/07 12:24" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5546 bp" 1) 
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COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 30-APR-2007" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "Promoter Eukaryotic (2 total)") 
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FEATURES             Location/Qualifiers
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
                     /note="E-tag"
     CDS             64..720
                     /vntifkey="4"
                     /label=YopE
     terminator      836..927
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit  ß-globin gene, incl. polyA"
     misc_feature    928..1284
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit  ß-globin gene"
     promoter        complement(1362..1444)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      1586..1661
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     terminator      1692..1766
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(1692..1766)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(1881..2282)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(2823..3683)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     promoter        3819..4196
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only"
     promoter        4202..5543
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     intron          4571..5495
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="intron of the chicken ß-actin/rabbit ß-globin hybrid promoter"
BASE COUNT     1184 a      1464 c      1544 g      1354 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gctaaaatat catcatttat ttctacatca ctgcccctgc cggcatcagt gtcaggatct 
      121 agcagcgtag gagaaatgtc tgggcgctca gtctcacagc aaaaaagtga tcaatatgca 
      181 aacaatctgg ccgggcgcac tgaaagccct cagggttcca gcttagccag ccgtatcatt 
      241 gagaggttat catcaatggc ccactctgtg attggattta tccaacgcat gttctcggag 
      301 gggagccata aaccggtggt gacaccagca ctcacgcctg cacaaatgcc aagccctacg 
      361 tctttcagtg atagtatcaa gcaacttgct gctgagacgc tgccaaaata catgcagcag 
      421 ttgagtagct tggatgcaga gacgctgcag aaaaatcatg accagttcgc cacgggcagc 
      481 ggccctcttc gtggcagtat cactcaatgc caagggctga tgcagttttg tggtggggaa 
      541 ttgcaagctg aggccagtgc cattttaaac acgcctgttt gtggtattcc cttctcgcag 
      601 tggggaactg ttggtggggc ggccagcgcg tacgtcgcca gtggcgttga tctaacgcag 
      661 gcagcaaatg agatcaaagg gctggggcaa cagatgcagc aattactgtc attgatgtga 
      721 cccgggacat taaacgcgtg ggtaccggat ccggcaattc actcctcagg tgcaggctgc 
      781 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct 
      841 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg 
      901 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact 
      961 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag 
     1021 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca 
     1081 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg 
     1141 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt 
     1201 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc 
     1261 tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt 
     1321 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 
     1381 gaagcataaa gtgtaaagcc tgggtgccta atgagtgagc taactcacat taattgcgtt 
     1441 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat 
     1501 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 
     1561 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 
     1621 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 
     1681 tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 
     1741 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 
     1801 tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag 
     1861 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 
     1921 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 
     1981 atcagggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 
     2041 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 
     2101 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 
     2161 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 
     2221 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 
     2281 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 
     2341 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 
     2401 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 
     2461 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 
     2521 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 
     2581 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 
     2641 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 
     2701 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 
     2761 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 
     2821 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 
     2881 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 
     2941 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 
     3001 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 
     3061 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 
     3121 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 
     3181 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 
     3241 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 
     3301 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 
     3361 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 
     3421 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 
     3481 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 
     3541 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 
     3601 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 
     3661 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 
     3721 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
     3781 gttccgcgca catttccccg aaaagtgcca cctgggtcga cattgattat tgactagtta 
     3841 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac 
     3901 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc 
     3961 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt 
     4021 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac 
     4081 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac 
     4141 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt 
     4201 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat 
     4261 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg 
     4321 gcccccccca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggaaaggtgc 
     4381 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg 
     4441 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgttgcgc gctgccttcc 
     4501 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt 
     4561 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt 
     4621 ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg 
     4681 ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc 
     4741 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg 
     4801 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg 
     4861 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg 
     4921 tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca 
     4981 cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg 
     5041 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg 
     5101 ctcgggggaa ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc 
     5161 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa 
     5221 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga 
     5281 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc 
     5341 gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg 
     5401 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg 
     5461 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg 
     5521 tgctgtctca tcattttggc aaagaa 
//
