ID pCAGGS-EYopE-R144A standard; circular DNA; ; 5546 BP. DE LMBP 4665. CC Accession number: LMBP 4665 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 25-NOV-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_marker 16..54 FT /vntifkey="22" FT /label=E-tag FT /note="E-tag" FT CDS 64..720 FT /vntifkey="4" FT /label=YopE-R144A FT /note="YopE mutant" FT terminator 836..927 FT /vntifkey="43" FT /label=ß-globin\polyA FT /note="3' UTR of the rabbit ß-globin gene, incl. polyA" FT misc_feature 928..1284 FT /vntifkey="21" FT /label=3'\FR\of\ß-globin FT /note="3' flanking region of the rabbit ß-globin gene" FT promoter complement(1362..1444) FT /vntifkey="30" FT /label=lac\promoter FT rep_origin 1586..1661 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT terminator 1692..1766 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(1692..1766) FT /vntifkey="43" FT /label=SV40\polyA\late FT rep_origin complement(1881..2282) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(2823..3683) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" FT promoter 3819..4196 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT /note="Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only" FT promoter 4202..5543 FT /vntifkey="29" FT /label=AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid promoter" FT intron 4571..5495 FT /vntifkey="15" FT /label=intron\AG\promoter FT /note="intron of the chicken ß-actin/rabbit ß-globin hybrid promoter" SQ Sequence 5546 BP; 1184 A; 1465 C; 1544 G; 1353 t; ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 60 gctaaaatat catcatttat ttctacatca ctgcccctgc cggcatcagt gtcaggatct 120 agcagcgtag gagaaatgtc tgggcgctca gtctcacagc aaaaaagtga tcaatatgca 180 aacaatctgg ccgggcgcac tgaaagccct cagggttcca gcttagccag ccgtatcatt 240 gagaggttat catcaatggc ccactctgtg attggattta tccaacgcat gttctcggag 300 gggagccata aaccggtggt gacaccagca ctcacgcctg cacaaatgcc aagccctacg 360 tctttcagtg atagtatcaa gcaacttgct gctgagacgc tgccaaaata catgcagcag 420 ttgagtagct tggatgcaga gacgctgcag aaaaatcatg accagttcgc cacgggcagc 480 ggccctcttg ccggcagtat cactcaatgc caagggctga tgcagttttg tggtggggaa 540 ttgcaagctg aggccagtgc cattttaaac acgcctgttt gtggtattcc cttctcgcag 600 tggggaactg ttggtggggc ggccagcgcg tacgtcgcca gtggcgttga tctaacgcag 660 gcagcaaatg agatcaaagg gctggggcaa cagatgcagc aattactgtc attgatgtga 720 cccgggacat taaacgcgtg ggtaccggat ccggcaattc actcctcagg tgcaggctgc 780 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct 840 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg 900 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact 960 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag 1020 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca 1080 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg 1140 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt 1200 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc 1260 tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt 1320 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 1380 gaagcataaa gtgtaaagcc tgggtgccta atgagtgagc taactcacat taattgcgtt 1440 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat 1500 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 1560 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 1620 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 1680 tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 1740 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 1800 tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag 1860 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 1920 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 1980 atcagggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 2040 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 2100 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 2160 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 2220 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 2280 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 2340 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 2400 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 2460 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 2520 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 2580 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 2640 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 2700 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 2760 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 2820 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 2880 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 2940 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 3000 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 3060 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 3120 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 3180 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 3240 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 3300 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 3360 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 3420 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 3480 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 3540 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 3600 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 3660 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 3720 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 3780 gttccgcgca catttccccg aaaagtgcca cctgggtcga cattgattat tgactagtta 3840 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac 3900 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc 3960 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt 4020 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac 4080 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac 4140 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt 4200 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat 4260 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg 4320 gcccccccca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggaaaggtgc 4380 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg 4440 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgttgcgc gctgccttcc 4500 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt 4560 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt 4620 ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg 4680 ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc 4740 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg 4800 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg 4860 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg 4920 tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca 4980 cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg 5040 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg 5100 ctcgggggaa ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc 5160 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa 5220 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga 5280 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc 5340 gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg 5400 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg 5460 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg 5520 tgctgtctca tcattttggc aaagaa 5546 //