LOCUS       pCAGGS-EYopT-C139S        5855 bp    DNA     circular     25-NOV-2008
DEFINITION  LMBP 4667.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4667
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 25-NOV-2008
            
COMMENT     VNTDATE|465734833|
COMMENT     VNTNAME|pCAGGS-EYopT-C139S|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-EYopT-C139S" 0 5855 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) (CStringList "BamHI" "EcoRI" "NotI" "XmaI" "NcoI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 5855 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 3 1057 0 1795 0 2132 0 0 
COMMENT            "") #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 4 0 896 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 1031 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 12 0 1888 0 4504 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "E-tag" 22 0 0 16 54 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "16..54") 
COMMENT        #6=(CFSignal (CObList) "YopT-C139S" 4 0 0 64 1029 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "64..1029") 
COMMENT        #7=(CFSignal (CObList) "\337-globin polyA" 43 0 0 1145 1236 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1145..1236") 
COMMENT        #8=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 1237 1593 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1237..1593") 
COMMENT        #9=(CFSignal (CObList) "lac promoter" 30 0 1 1671 1753 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1671..1753)") 
COMMENT        #10=(CFSignal (CObList) "SV40 ori" 33 0 0 1895 1970 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1895..1970") 
COMMENT        #11=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2001 2075 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2001..2075") 
COMMENT        #12=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2001 2075 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2001..2075)") 
COMMENT        #13=(CFSignal (CObList) "pMB1 ori" 33 0 1 2190 2591 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2190..2591)") 
COMMENT        #14=(CFSignal (CObList) "ampicillin" 4 0 1 3132 3992 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3132..3992)") 
COMMENT        #15=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4128 4505 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4128..4505") 
COMMENT        #16=(CFSignal (CObList) "AG promoter" 29 0 0 4511 5852 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4511..5852") 
COMMENT        #17=(CFSignal (CObList) "intron AG promoter" 15 0 0 4880 5804 0 
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COMMENT       (CObList) (CObList) (CObList) 
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COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #19=(CFSignal (CObList) "Last modified: 25-NOV-2008" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
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COMMENT                          "DNA Plasmid 'pCAGGS-EYopT-C139S'" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4667" 
COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 12/14/07 10:47" 1) 
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COMMENT                          "Last Modified: 11/25/08 04:10" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5855 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "Original Source Database: GenBank" 1) 
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COMMENT                          "Modification Date in the Original DB: 14-DEC-2007" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                   #73=(CLinePar 
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COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                    "Restriction/Methylation Map")))) 
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FEATURES             Location/Qualifiers
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
                     /note="E-tag"
     CDS             64..1029
                     /vntifkey="4"
                     /label=YopT-C139S
                     /note="mutated Yersinia enterocolitica protein coding sequence"
     terminator      1145..1236
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     misc_feature    1237..1593
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit ß-globin gene"
     promoter        complement(1671..1753)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      1895..1970
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     terminator      2001..2075
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(2001..2075)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(2190..2591)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(3132..3992)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     promoter        4128..4505
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only"
     promoter        4511..5852
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     intron          4880..5804
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="intron of chicken ß-actin/rabbit ß-globin hybrid promoter"
BASE COUNT     1310 a      1500 c      1586 g      1459 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gctgacagta ttcacggaca ctaccatatc caactatcga attattctgc cggtgaaaac 
      121 cttcaatcag ctacactcac cgaaggggtg attggcgcac accgagtgaa agtggaaaca 
      181 gcactgtcac actcaaaccg gcagaaaaag ttatcagcta ccataaaaca taaccagtca 
      241 agccgttcta tgctggatag aaagttgacc agcgacggca aagctaacca acgaagcagc 
      301 tttaccttca gtatgattat gtatcgcatg atacattttg tactcagtac tcgtgtgccc 
      361 gcggtgagag agtctgttgc aaattacgga ggtaacatca atttcaagtt tgctcagacc 
      421 aaaggggctt ttcttcatca aataataaaa cattcagaca ccgctagcgg tgtcagtgag 
      481 gctttatgtg cacattggat ttggagtcat gcacagggcc aaagcttatt tgaccagctc 
      541 tatgttggcg ggcgtaaagg gaaattccag atcgatacac tttactcaat taaacagttg 
      601 caaatagatg gttgtaaagc agacgttgat caagatgagg taacactaga ttggttcaag 
      661 aaaaagggca tatcagaacg tatgattgaa cggcattgct tactgcgtcc agttgatgtt 
      721 actggtacga cggaatcaga agggccggat caattattaa acgctatcct tgatactcat 
      781 gggataggtt acggttataa aaaaatatat ctctcaggcc aaatgtcagc ccacgccata 
      841 gcggcgtatg tcaacgaaaa gagtggtgtt actttcttcg atcccaattt cggtgaattc 
      901 cacttttctg ataaggaaaa gttccgcaaa tggtttacta actcattctg ggaaaattct 
      961 atgtatcatt atcctctggg ggtggggcag cgttttagcg tgttaacatt tgactccaag 
     1021 gaggtttgac ccgggacatt aaacgcgtgg gtaccggatc cggcaattca ctcctcaggt 
     1081 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca 
     1141 ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct 
     1201 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg 
     1261 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt 
     1321 ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata 
     1381 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc 
     1441 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc 
     1501 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc 
     1561 agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt 
     1621 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 
     1681 tacgagccgg aagcataaag tgtaaagcct gggtgcctaa tgagtgagct aactcacatt 
     1741 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg 
     1801 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 
     1861 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 
     1921 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 
     1981 ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac aaataaagca 
     2041 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 
     2101 ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa tcggccaacg 
     2161 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 
     2221 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 
     2281 atccacagaa tcagggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 
     2341 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 
     2401 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 
     2461 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 
     2521 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 
     2581 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 
     2641 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 
     2701 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 
     2761 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 
     2821 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 
     2881 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 
     2941 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 
     3001 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 
     3061 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 
     3121 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 
     3181 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 
     3241 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 
     3301 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 
     3361 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 
     3421 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 
     3481 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 
     3541 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 
     3601 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 
     3661 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 
     3721 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 
     3781 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 
     3841 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 
     3901 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 
     3961 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 
     4021 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 
     4081 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtcgac attgattatt 
     4141 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt 
     4201 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc 
     4261 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg 
     4321 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat 
     4381 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca 
     4441 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat 
     4501 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc 
     4561 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg 
     4621 gggggggggg ccccccccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg 
     4681 gaaaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag 
     4741 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgttgcgcg 
     4801 ctgccttccc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact 
     4861 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta 
     4921 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct 
     4981 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt 
     5041 ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc 
     5101 ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg 
     5161 gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg 
     5221 agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt 
     5281 tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc 
     5341 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc 
     5401 cggggagggc tcgggggaag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg 
     5461 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt 
     5521 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc 
     5581 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt 
     5641 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct 
     5701 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta 
     5761 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc 
     5821 tggttattgt gctgtctcat cattttggca aagaa 
//
