ID pCAGGS-EYopT-C139S standard; circular DNA; ; 5855 BP. DE LMBP 4667. CC Accession number: LMBP 4667 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 25-NOV-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_marker 16..54 FT /vntifkey="22" FT /label=E-tag FT /note="E-tag" FT CDS 64..1029 FT /vntifkey="4" FT /label=YopT-C139S FT /note="mutated Yersinia enterocolitica protein coding sequence" FT terminator 1145..1236 FT /vntifkey="43" FT /label=ß-globin\polyA FT /note="3' UTR of the rabbit ß-globin gene, incl. polyA" FT misc_feature 1237..1593 FT /vntifkey="21" FT /label=3'\FR\of\ß-globin FT /note="3' flanking region of the rabbit ß-globin gene" FT promoter complement(1671..1753) FT /vntifkey="30" FT /label=lac\promoter FT rep_origin 1895..1970 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT terminator 2001..2075 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(2001..2075) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT rep_origin complement(2190..2591) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(3132..3992) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" FT promoter 4128..4505 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT /note="Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only" FT promoter 4511..5852 FT /vntifkey="29" FT /label=AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid promoter" FT intron 4880..5804 FT /vntifkey="15" FT /label=intron\AG\promoter FT /note="intron of chicken ß-actin/rabbit ß-globin hybrid promoter" SQ Sequence 5855 BP; 1310 A; 1500 C; 1586 G; 1459 t; ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 60 gctgacagta ttcacggaca ctaccatatc caactatcga attattctgc cggtgaaaac 120 cttcaatcag ctacactcac cgaaggggtg attggcgcac accgagtgaa agtggaaaca 180 gcactgtcac actcaaaccg gcagaaaaag ttatcagcta ccataaaaca taaccagtca 240 agccgttcta tgctggatag aaagttgacc agcgacggca aagctaacca acgaagcagc 300 tttaccttca gtatgattat gtatcgcatg atacattttg tactcagtac tcgtgtgccc 360 gcggtgagag agtctgttgc aaattacgga ggtaacatca atttcaagtt tgctcagacc 420 aaaggggctt ttcttcatca aataataaaa cattcagaca ccgctagcgg tgtcagtgag 480 gctttatgtg cacattggat ttggagtcat gcacagggcc aaagcttatt tgaccagctc 540 tatgttggcg ggcgtaaagg gaaattccag atcgatacac tttactcaat taaacagttg 600 caaatagatg gttgtaaagc agacgttgat caagatgagg taacactaga ttggttcaag 660 aaaaagggca tatcagaacg tatgattgaa cggcattgct tactgcgtcc agttgatgtt 720 actggtacga cggaatcaga agggccggat caattattaa acgctatcct tgatactcat 780 gggataggtt acggttataa aaaaatatat ctctcaggcc aaatgtcagc ccacgccata 840 gcggcgtatg tcaacgaaaa gagtggtgtt actttcttcg atcccaattt cggtgaattc 900 cacttttctg ataaggaaaa gttccgcaaa tggtttacta actcattctg ggaaaattct 960 atgtatcatt atcctctggg ggtggggcag cgttttagcg tgttaacatt tgactccaag 1020 gaggtttgac ccgggacatt aaacgcgtgg gtaccggatc cggcaattca ctcctcaggt 1080 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca 1140 ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct 1200 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg 1260 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt 1320 ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata 1380 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc 1440 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc 1500 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc 1560 agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt 1620 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 1680 tacgagccgg aagcataaag tgtaaagcct gggtgcctaa tgagtgagct aactcacatt 1740 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg 1800 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 1860 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 1920 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 1980 ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac aaataaagca 2040 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 2100 ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa tcggccaacg 2160 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 2220 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 2280 atccacagaa tcagggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 2340 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 2400 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 2460 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 2520 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 2580 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 2640 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 2700 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 2760 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 2820 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 2880 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 2940 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 3000 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 3060 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 3120 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 3180 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 3240 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 3300 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 3360 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 3420 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 3480 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 3540 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 3600 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 3660 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 3720 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 3780 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 3840 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 3900 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 3960 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 4020 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 4080 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtcgac attgattatt 4140 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt 4200 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc 4260 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg 4320 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat 4380 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca 4440 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat 4500 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc 4560 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg 4620 gggggggggg ccccccccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg 4680 gaaaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag 4740 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgttgcgcg 4800 ctgccttccc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact 4860 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta 4920 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct 4980 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt 5040 ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc 5100 ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg 5160 gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg 5220 agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt 5280 tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc 5340 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc 5400 cggggagggc tcgggggaag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg 5460 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt 5520 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc 5580 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt 5640 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct 5700 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta 5760 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc 5820 tggttattgt gctgtctcat cattttggca aagaa 5855 //