ID pdcDNA-FLAGMyc standard; circular DNA; UNA; 7087 BP. DE LMBP 4705. CC Accession number: LMBP 4705 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 15-JUN-2012 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 27..610 FT /vntifkey="29" FT /label=hCMV-IE\promoter\and\enhancer FT promoter 655..671 FT /vntifkey="30" FT /label=T7\promoter FT misc_marker 696..719 FT /vntifkey="22" FT /label=FLAG\epitope FT attenuator complement(729..853) FT /vntifkey="0" FT /label=attR1 FT promoter 861..913 FT /vntifkey="30" FT /label=lacUV5\promoter FT CDS 962..1621 FT /vntifkey="4" FT /label=chloramphenicol\resistance FT CDS 1963..2268 FT /vntifkey="4" FT /label=ccdB FT attenuator 2309..2433 FT /vntifkey="0" FT /label=attR2 FT misc_marker 2438..2467 FT /vntifkey="22" FT /label=Myc\epitope FT promoter complement(2506..2522) FT /vntifkey="30" FT /label=SP6\promoter FT terminator 2548..2772 FT /vntifkey="43" FT /label=BGH\polyA FT rep_origin 2818..3246 FT /vntifkey="33" FT /label=f1\ori FT promoter 3251..3460 FT /vntifkey="29" FT /label=SV40\early\promoter FT rep_origin 3417..3492 FT /vntifkey="33" FT /label=SV40\ori FT CDS 3584..4378 FT /vntifkey="4" FT /label=Tn5\neomycin\resistance FT terminator 4552..4626 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(4552..4626) FT /vntifkey="43" FT /label=SV40\polyA\late FT promoter complement(4765..4848) FT /vntifkey="30" FT /label=lac\promoter FT rep_origin complement(4940..5342) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(5883..6743) FT /vntifkey="4" FT /label=ampicillin\resistance SQ Sequence 7087 BP; 1729 A; 1743 C; 1772 G; 1843 t; cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc 60 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt 120 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 180 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg 240 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 300 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt 360 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg 420 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc 480 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg 540 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat 600 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac 660 gactcactat agggagaccc aagcttacca ccatggacac taaggatgac gatgacaaag 720 cggccatcac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca 780 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata 840 tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc 900 gtataatgtg tggattttga gttaggatcc ggcgagattt tcaggagcta aggaagctaa 960 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga 1020 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga 1080 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat 1140 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg 1200 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga 1260 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata 1320 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga 1380 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt 1440 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg 1500 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg atggcttcca 1560 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta 1620 aacgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt 1680 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtgtgct 1740 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat 1800 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc 1860 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg 1920 aaatgaacgg ctcttttgct gacgagaaca gggactggtg aaatgcagtt taaggtttac 1980 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac 2040 acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc 2100 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc 2160 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc 2220 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc 2280 cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta 2340 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta 2400 cgtttctcgt tcagctttct tgtacaaagt ggtgatcgaa cagaagctga tatcggaaga 2460 ggacctgaac tagtaactcg agcatgcatc tagagggccc tattctatag tgtcacctaa 2520 atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt 2580 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat 2640 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg 2700 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg 2760 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg 2820 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 2880 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 2940 tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg 3000 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 3060 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 3120 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 3180 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 3240 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc 3300 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac 3360 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 3420 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta 3480 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc 3540 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg 3600 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 3660 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 3720 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg 3780 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 3840 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 3900 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 3960 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 4020 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 4080 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt 4140 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 4200 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 4260 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 4320 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc 4380 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc 4440 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc 4500 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt 4560 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 4620 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 4680 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg 4740 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 4800 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 4860 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 4920 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 4980 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 5040 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 5100 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 5160 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 5220 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 5280 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc 5340 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 5400 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 5460 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 5520 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 5580 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 5640 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 5700 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 5760 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 5820 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 5880 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 5940 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 6000 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 6060 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 6120 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 6180 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 6240 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 6300 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 6360 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 6420 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 6480 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 6540 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 6600 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 6660 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 6720 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 6780 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 6840 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg 6900 atcccctatg gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc 6960 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa 7020 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc 7080 tgcttcg 7087 //