LOCUS       pCAGGS-E-hCASP-1 6048 bp dsDNACircular
DEFINITION  LMBP 4715.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     misc_difference 11..49
                     /note="E-tag"
                     /label=E-tag
     CDS             62..1276
                     /note="human caspase-1 coding sequence"
                     /label=hCASP-1
     CDS             complement(3320..4180)
                     /note="antibiotic resistance gene; coding sequence"
                     /label=AMP
     promoter        4316..4693
                     /note="human CMV-IE enhancer"
                     /label=hCMV\enhancer
     promoter        complement(1859..1941)
                     /note="E. coli lac operon promoter"
                     /label=lac\promoter
     rep_origin      2083..2158
                     /note="bidirectional origin of SV40"
                     /label=SV40\ori
     terminator      2189..2263
                     /note="polyA signal of the SV40 early region"
                     /label=SV40\polyA\early
     terminator      complement(2189..2263)
                     /note="polyA signal of the SV40 late region"
                     /label=SV40\polyA\late
     rep_origin      complement(2378..2779)
                     /note="origin plasmid"
                     /label=pMB1\ori
     terminator      1333..1424
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
                     /label=ß-globin\polyA
     misc_feature    1425..1781
                     /note="3' flanking region of the rabbit ß-globin gene"
                     /label=3FR\of\ß-globin
     promoter        4699..6040
                     /note="chicken ß-actin/ rabbit ß-globin hybrid promoter"
                     /label=AG\promoter
     intron          5068..5992
                     /note="chicken ß-actin/ rabbit ß-globin hybrid intron"
                     /label=intron\AG\promoter
ORIGIN
        1 ttccaccatg ggtgcgccgg tgccgtatcc ggacccgctg gaaccgcgtg cggccgcgcc 
       61 gatggccgac aaggtcctga aggagaagag aaagctgttt atccgttcca tgggtgaagg 
      121 tacaataaat ggcttactgg atgaattatt acagacaagg gtgctgaaca aggaagagat 
      181 ggagaaagta aaacgtgaaa atgctacagt tatggataag acccgagctt tgattgactc 
      241 cgttattccg aaaggggcac aggcatgcca aatttgcatc acatacattt gtgaagaaga 
      301 cagttacctg gcagggacgc tgggactctc agcagatcaa acatctggaa attaccttaa 
      361 tatgcaagac tctcaaggag tactttcttc ctttccagct cctcaggcag tgcaggacaa 
      421 cccagctatg cccacatcct caggctcaga agggaatgtc aagctttgct ccctagaaga 
      481 agctcaaagg atatggaaac aaaagtcggc agagatttat ccaataatgg acaagtcaag 
      541 ccgcacacgt cttgctctca ttatctgcaa tgaagaattt gacagtattc ctagaagaac 
      601 tggagctgag gttgacatca caggcatgac aatgctgcta caaaatctgg ggtacagcgt 
      661 agatgtgaaa aaaaatctca ctgcttcgga catgactaca gagctggagg catttgcaca 
      721 ccgcccagag cacaagacct ctgacagcac gttcctggtg ttcatgtctc atggtattcg 
      781 ggaaggcatt tgtgggaaga aacactctga gcaagtccca gatatactac aactcaatgc 
      841 aatctttaac atgttgaata ccaagaactg cccaagtttg aaggacaaac cgaaggtgat 
      901 catcatccag gcctgccgtg gtgacagccc tggtgtggtg tggtttaaag attcagtagg 
      961 agtttctgga aacctatctt taccaactac agaagagttt gaggatgatg ctattaagaa 
     1021 agcccacata gagaaggatt ttatcgcttt ctgctcttcc acaccagata atgtttcttg 
     1081 gagacatccc acaatgggct ctgtttttat tggaagactc attgaacata tgcaagaata 
     1141 tgcctgttcc tgtgatgtgg aggaaatttt ccgcaaggtt cgattttcat ttgagcagcc 
     1201 agatggtaga gcgcagatgc ccaccactga aagagtgact ttgacaagat gtttctacct 
     1261 cttcccagga cattaaaata aggaaactgt atgaatgtct gtgggcagga agtgaagaga 
     1321 tcgttctgta aagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt 
     1381 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat 
     1441 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat 
     1501 gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt 
     1561 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat 
     1621 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct 
     1681 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga 
     1741 ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag 
     1801 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 
     1861 acacaacata cgagccggaa gcataaagtg taaagcctgg gtgcctaatg agtgagctaa 
     1921 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 
     1981 cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 
     2041 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 
     2101 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 
     2161 ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa 
     2221 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 
     2281 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc 
     2341 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 
     2401 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 
     2461 atacggttat ccacagaatc agggataacg caggaaagaa catgtgagca aaaggccagc 
     2521 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 
     2581 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 
     2641 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 
     2701 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 
     2761 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 
     2821 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 
     2881 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 
     2941 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 
     3001 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 
     3061 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 
     3121 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 
     3181 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 
     3241 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 
     3301 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 
     3361 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 
     3421 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 
     3481 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 
     3541 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 
     3601 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 
     3661 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 
     3721 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 
     3781 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 
     3841 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 
     3901 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 
     3961 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 
     4021 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 
     4081 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 
     4141 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 
     4201 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 
     4261 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcgacat 
     4321 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat 
     4381 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 
     4441 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 
     4501 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg 
     4561 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 
     4621 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 
     4681 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc 
     4741 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg 
     4801 gcgggggggg ggggggggcc ccccccaggc ggggcggggc ggggcgaggg gcggggcggg 
     4861 gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt 
     4921 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc 
     4981 gttgcgcgct gccttccccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg 
     5041 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc 
     5101 tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt 
     5161 gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt 
     5221 gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg 
     5281 cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt 
     5341 gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg 
     5401 ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct 
     5461 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg 
     5521 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg 
     5581 cctcgggccg gggagggctc gggggaaggg gcgcggcggc ccccggagcg ccggcggctg 
     5641 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg 
     5701 acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc 
     5761 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct 
     5821 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg 
     5881 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg 
     5941 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg 
     6001 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgaa 
//
