LOCUS       pAT153MIL2F2            4664 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 4731.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pAT153MIL2F2|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAT153MIL2F2" 0 4664 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
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COMMENT        #6=(CFSignal (CObList) "mIL2" 4 0 1 3333 3842 0 (CStringList) 
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COMMENT        #7=(CFSignal (CObList) "5UTR" 21 0 1 3843 3889 0 (CStringList) 
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COMMENT                         "DNA 'pAT153MIL2F2'" 1) 
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COMMENT                         "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                         1) 
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COMMENT                         "length: 4664 bp" 1) 
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COMMENT                                       "antibiotic resistance gene; coding sequence" 
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COMMENT                                       "Start: 3333  End: 3842 (Complementary)" 
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COMMENT                                       "mouse interleukin 2 coding sequence" 1) 
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FEATURES             Location/Qualifiers
     CDS             86..1276
                     /vntifkey="4"
                     /label=TET
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      complement(1647..2049)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     misc_feature    complement(2951..3332)
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of the mouse interleukin 2 gene"
     CDS             complement(3333..3842)
                     /vntifkey="4"
                     /label=mIL2
                     /note="mouse interleukin 2 coding sequence"
     misc_feature    complement(3843..3889)
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR of the mouse interleukin 2 gene"
BASE COUNT     1090 a      1206 c      1176 g      1192 t 
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 
      181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 
      241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 
      301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 
      361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac 
      421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca 
      481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg 
      541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct 
      601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat 
      661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt 
      721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct 
      781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct 
      841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa 
      901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt 
      961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc 
     1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca 
     1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc 
     1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat 
     1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg 
     1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga 
     1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac 
     1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg 
     1441 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg 
     1501 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc 
     1561 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg 
     1621 taaagtctgg aaacgcggaa gtcagcgctc ttccgcttcc tcgctcactg actcgctgcg 
     1681 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 
     1741 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 
     1801 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 
     1861 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 
     1921 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 
     1981 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 
     2041 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 
     2101 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 
     2161 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 
     2221 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 
     2281 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 
     2341 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 
     2401 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 
     2461 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 
     2521 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 
     2581 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 
     2641 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 
     2701 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 
     2761 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 
     2821 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 
     2881 tttgcgcaac gttgttgcca ttgctgcagg gggggggggg gggggggggt tttttttttt 
     2941 tttttttttt agaggagagc tttatttctt gaaaacactg attaacatag agttcacagg 
     3001 aataactgag aagttatttt agcgcttact ttgtgctgtc ctaaaaatga cagacatctg 
     3061 agcttattta tatttgaatc atctaaatac ttttattaat gagtctacct acacatgata 
     3121 tttaacaatt caatataata aataatttca gataaatagt ttaaaacatt tttgagccct 
     3181 tggggcttac aaaaagaatc tttaaagatc catatttatc atctgaagac tagtagttac 
     3241 aaaagatagt aaacaataca tccaaaaata aattaaagtt aaatatttaa ataaatagag 
     3301 agccttatgt gttgtaagca ggaggtacat agttattgag ggcttgttga gatgatgctt 
     3361 tgacagaagg ctatccatct cctcagaaag tccaccacag ttgctgactc atcatcgaat 
     3421 tggcactcaa atgtgttgtc agagcccttt agttttacaa cagttactct gatattgctg 
     3481 atgaaattct cagcatcttc caattgaaag cttttgcttt gagtcaaatc cagaacatgc 
     3541 cgcagaggtc caagttcatc ttctaggcac tgaagatctt tcaattctgt ggcctgcttg 
     3601 ggcaagtaaa atttgaaggt gagcatcctg gggagtttca ggttcctgta attctccatc 
     3661 ctgctcagga gctcctgtag gtccatcaac agctgctcca ggtgctgctg ctgctgctgc 
     3721 tgctgctgct gctgctgctg tgcttccgct gtagagcttg aagtggagct tgaagtgggt 
     3781 gcgctgttga caaggagcac aagtgtcaat gtgacacagg atgcgagctg catgctgtac 
     3841 atgcctgcag gacttgaggt cactgtgagg agtgattagc aagggtgatc cccccccccc 
     3901 cccccccccc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 
     3961 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 
     4021 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 
     4081 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 
     4141 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 
     4201 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 
     4261 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 
     4321 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 
     4381 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 
     4441 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 
     4501 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 
     4561 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 
     4621 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaa 
//
