ID pAS2hBID standard; circular DNA; ; 8988 BP. DE LMBP 4756. CC Accession number: LMBP 4756 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 23-OCT-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS complement(3855..4304) FT /vntifkey="4" FT /label=CYH2 FT rep_origin complement(2661..3116) FT /vntifkey="33" FT /label=f1\ori FT CDS 1884..2558 FT /vntifkey="4" FT /label=TRP1 FT misc_marker 5957..5983 FT /vntifkey="22" FT /label=HA\epitope FT CDS 5501..5941 FT /vntifkey="4" FT /label=GALbd FT promoter 5075..5478 FT /vntifkey="29" FT /label=ADH1\promoter FT CDS 6011..6598 FT /vntifkey="4" FT /label=hBID FT CDS complement(7998..8858) FT /vntifkey="4" FT /label=ampicillin FT rep_origin complement(7055..7457) FT /vntifkey="33" FT /label=pMB1\ori FT terminator 6626..6727 FT /vntifkey="43" FT /label=ADH1\terminator FT terminator complement(3312..3586) FT /vntifkey="43" FT /label=CYC1\terminator FT rep_origin complement(2..982) FT /vntifkey="33" FT /label=2mu\ori FT /note="2µ origin, incl. region conferring stability (STB)" FT promoter complement(6880..6963) FT /vntifkey="30" FT /label=lac\promoter FT terminator complement(1060..1164) FT /vntifkey="43" FT /label=FLP\terminator SQ Sequence 8988 BP; 2493 A; 1920 C; 2050 G; 2525 t; gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 60 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 120 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 180 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 240 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 300 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 360 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 420 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 480 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 540 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 600 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 660 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 720 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 780 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 840 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 900 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 960 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 1020 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 1080 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 1140 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 1200 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 1260 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 1320 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 1380 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 1440 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 1500 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 1560 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 1620 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 1680 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 1740 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 1800 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 1860 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 1920 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 1980 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 2040 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 2100 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 2160 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg 2220 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag 2280 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg 2340 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct 2400 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa 2460 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta 2520 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat 2580 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa 2640 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 2700 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 2760 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 2820 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 2880 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 2940 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 3000 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 3060 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg 3120 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 3180 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 3240 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga 3300 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt 3360 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata 3420 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag 3480 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc 3540 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt 3600 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca 3660 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 3720 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga 3780 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct 3840 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct 3900 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg aattctaccc 3960 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta 4020 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc 4080 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc 4140 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg 4200 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag 4260 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat 4320 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag 4380 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata 4440 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg 4500 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat 4560 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt 4620 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc 4680 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca 4740 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg 4800 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt 4860 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca 4920 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa 4980 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt 5040 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt 5100 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac 5160 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 5220 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc 5280 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt 5340 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat 5400 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag 5460 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat 5520 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa 5580 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc 5640 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct 5700 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca 5760 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt 5820 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat 5880 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc 5940 gccggaattc atggcttacc catacgatgt tccagattac gctagcttgg gtggtcatat 6000 ggccatggcg atggactgtg aggtcaacaa cggttccagc ctcagggatg agtgcatcac 6060 aaacctactg gtgtttggct tcctccaaag ctgttctgac aacagcttcc gcagagagct 6120 ggacgcactg ggccacgagc tgccagtgct ggctccccag tgggagggct acgatgagct 6180 gcagactgat ggcaaccgca gcagccactc ccgcttggga agaatagagg cagattctga 6240 aagtcaagaa gacatcatcc ggaatattgc caggcacctc gcccaggtcg gggacagcat 6300 ggaccgtagc atccctccgg gcctggtgaa cggcctggcc ctgcagctca ggaacaccag 6360 ccggtcggag gaggaccgga acagggacct ggccactgcc ctggagcagc tgctgcaggc 6420 ctaccctaga gacatggaga aggagaagac catgctggtg ctggccctgc tgctggccaa 6480 gaaggtggcc agtcacacgc cgtccttgct ccgtgatgtc tttcacacaa cagtgaattt 6540 tattaaccag aacctacgca cctacgtgag gagcttagcc agaaatggga tggactgagt 6600 cgacctgcag ccaagctaat tccgggcgaa tttcttatga tttatgattt ttattattaa 6660 ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg 6720 aaaattcttg ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagca 6780 tgaggtcgct cttattgacc acacctctac cggcatgcaa gcttggcgta atcatggtca 6840 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 6900 agcataaagt gtaaagcctg gggtgcctaa tgagtgaggt aactcacatt aattgcgttg 6960 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctggatta atgaatcggc 7020 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 7080 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 7140 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 7200 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 7260 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 7320 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 7380 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 7440 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 7500 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 7560 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 7620 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 7680 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 7740 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 7800 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 7860 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 7920 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 7980 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 8040 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 8100 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 8160 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 8220 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 8280 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 8340 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 8400 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 8460 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 8520 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 8580 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 8640 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 8700 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 8760 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 8820 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 8880 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 8940 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacct 8988 //