LOCUS       pAS2-1htBID             8781 bp    DNA     circular     23-OCT-2007
DEFINITION  LMBP 4759.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4759
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 23-OCT-2007
            
COMMENT     VNTNAME|pAS2.1htBID|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAS2.1htBID" 0 8781 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "Alw44I" "BglI" "BglII" "Eco81I" "EcoRI" "NcoI" "NheI" 
COMMENT         "PvuII" "SalI" "SspI" "XhoI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 8781 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #3=(CRSite (CStringList) "Alw44I" "gtgcac" 2 0 4 1590 0 2595 0 7286 0 
COMMENT            8532 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 2 3134 0 7985 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "BglII" "agatct" 2 0 1 3969 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "Eco81I" "cctnagg" 3 0 1 2126 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 5977 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "NheI" "gctagc" 2 0 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "PvuII" "cagctg" 4 0 3 3191 0 6203 0 6796 0 0 
COMMENT             "") #11=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 6393 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "SspI" "aatatt" 4 0 5 880 0 2678 0 5443 0 6059 
COMMENT             0 8669 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 3297 0 5719 0 0 "")) 
COMMENT       (CObList 
COMMENT        #14=(CFSignal (CObList) "GALbd" 4 0 0 5501 5941 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5501..5941") 
COMMENT        #15=(CFSignal (CObList) "CYH2" 4 0 1 3855 4304 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3855..4304)") 
COMMENT        #16=(CFSignal (CObList) "CYC1 terminator" 43 0 1 3312 3586 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3312..3586)") 
COMMENT        #17=(CFSignal (CObList) "f1 ori" 33 0 1 2661 3116 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2661..3116)") 
COMMENT        #18=(CFSignal (CObList) "TRP1" 4 0 0 1884 2558 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1884..2558") 
COMMENT        #19=(CFSignal (CObList) "ADH1 terminator" 43 0 0 6419 6520 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "6419..6520") 
COMMENT        #20=(CFSignal (CObList) "pMB1 ori" 33 0 1 6848 7250 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(6848..7250)") 
COMMENT        #21=(CFSignal (CObList) "ampicillin" 4 0 1 7791 8651 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(7791..8651)") 
COMMENT        #22=(CFSignal (CObList) "2mu ori" 33 0 1 2 982 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2..982)") 
COMMENT        #23=(CFSignal (CObList) "htBID" 4 0 0 5984 6391 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5984..6391") 
COMMENT        #24=(CFSignal (CObList) "FLP terminator" 43 0 1 1060 1164 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #25=(CFSignal (CObList) "lac promoter" 30 0 1 6673 6756 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #26=(CFSignal (CObList) "ADH1 promoter" 29 0 0 5075 5478 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5075..5478")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList 
COMMENT        #27=(CFSignal (CObList) "LMBP 4759" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #28=(CFSignal (CObList) "Last modified: 23-OCT-2007" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
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COMMENT              #30=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pAS2.1htBID" 2) 5 
COMMENT                   "" 0 4) 
COMMENT              #31=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 1 178) 
COMMENT                    (CObjectList 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pAS2.1htBID'" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4759" 
COMMENT                          1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 10/23/07 04:54" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 8781 bp" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #40=(CFolderPar 
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COMMENT                    (CObjectList 
COMMENT                     #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #42=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #43=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
COMMENT                          1))) "Original Author") 
COMMENT              #45=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #46=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #47=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #48=(CLinePar (CParagraph 0 (15 #27# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 4759") 
COMMENT                     #49=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #50=(CLinePar (CParagraph 0 (15 #28# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 23-OCT-2007"))) 
COMMENT                   "Annotations") 
COMMENT              #51=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #52=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #53=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1884 (3 #18# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1884  End: 2558" 1) 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1884..2558" 1))) "TRP1") 
COMMENT                            #57=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3855 (3 #15# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #58=(CLinePar 
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COMMENT                                        "Start: 3855  End: 4304 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3855..4304)" 1))) "CYH2") 
COMMENT                            #61=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5501 (3 #14# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5501  End: 5941" 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5501..5941" 1))) "GALbd") 
COMMENT                            #65=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5984 (3 #23# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5984  End: 6391" 1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5984..6391" 1))) "htBID") 
COMMENT                            #69=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 7791 (3 #21# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7791  End: 8651 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(7791..8651)" 1))) 
COMMENT                                 "ampicillin"))) "CDS (5 total)") 
COMMENT                     #73=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 1 178) 
COMMENT                           (CObjectList 
COMMENT                            #74=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5075 (3 #26# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5075  End: 5478" 1) 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5075..5478" 1))) "ADH1 promoter"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
COMMENT                     #78=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #79=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 6673 (3 #25# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6673  End: 6756 (Complementary)" 
COMMENT                                        1))) "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
COMMENT                     #81=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #82=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2     End: 982  (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "2\265 origin, incl. region conferring stability (STB)" 
COMMENT                                        1) 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2..982)" 1))) "2mu ori") 
COMMENT                            #87=(CFolderPar 
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COMMENT                                        "Start: 2661  End: 3116 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2661..3116)" 1))) 
COMMENT                                 "f1 ori") 
COMMENT                            #91=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #92=(CLinePar 
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COMMENT                                        "Start: 6848  End: 7250 (Complementary)" 
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COMMENT                                   #93=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(6848..7250)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (3 total)") 
COMMENT                     #95=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                        "Start: 1060  End: 1164 (Complementary)" 
COMMENT                                        1))) "FLP terminator") 
COMMENT                            #98=(CFolderPar 
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COMMENT                                   #100=(CLinePar 
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COMMENT                                   #101=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   complement(3312..3586)" 1))) 
COMMENT                                 "CYC1 terminator") 
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COMMENT                                    #103=(CLinePar 
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COMMENT                                          "Start: 6419  End: 6520" 1) 
COMMENT                                    #104=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                                    #105=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   6419..6520" 1))) "ADH1 terminator"))) 
COMMENT                          "Terminator (3 total)"))) "Feature Map") 
COMMENT              #106=(CFolderPar 
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COMMENT                     (CObjectList 
COMMENT                      #107=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "EcoRI: 0 sites") 1 5 "GAATTC" 
COMMENT                            "CTTAAG") 
COMMENT                      #108=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "NheI: 0 sites") 1 5 "GCTAGC" 
COMMENT                            "CGATCG") 
COMMENT                      #109=(CRSFolderPar 
COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3969 " 1))))) "BglII: 1 site") 
COMMENT                            1 5 "AGATCT" "TCTAGA") 
COMMENT                      #112=(CRSFolderPar 
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COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
COMMENT                                      #114=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2126 " 1))))) "Eco81I: 1 site") 
COMMENT                            2 5 "CCTNAGG" "GGANTCC") 
COMMENT                      #115=(CRSFolderPar 
COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
COMMENT                                      #117=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #8# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 5977 " 1))))) "NcoI: 1 site") 
COMMENT                            1 5 "CCATGG" "GGTACC") 
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FEATURES             Location/Qualifiers
     CDS             5501..5941
                     /vntifkey="4"
                     /label=GALbd
     promoter        5075..5478
                     /vntifkey="29"
                     /label=ADH1\promoter
     CDS             complement(3855..4304)
                     /vntifkey="4"
                     /label=CYH2
     terminator      complement(3312..3586)
                     /vntifkey="43"
                     /label=CYC1\terminator
     rep_origin      complement(2661..3116)
                     /vntifkey="33"
                     /label=f1\ori
     CDS             1884..2558
                     /vntifkey="4"
                     /label=TRP1
     terminator      6419..6520
                     /vntifkey="43"
                     /label=ADH1\terminator
     rep_origin      complement(6848..7250)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(7791..8651)
                     /vntifkey="4"
                     /label=ampicillin
     rep_origin      complement(2..982)
                     /vntifkey="33"
                     /label=2mu\ori
                     /note="2µ origin, incl. region conferring stability (STB)"
     CDS             5984..6391
                     /vntifkey="4"
                     /label=htBID
     terminator      complement(1060..1164)
                     /vntifkey="43"
                     /label=FLP\terminator
     promoter        complement(6673..6756)
                     /vntifkey="30"
                     /label=lac\promoter
BASE COUNT     2448 a      1866 c      1986 g      2481 t 
ORIGIN
        1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 
       61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 
      121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 
      181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 
      241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 
      301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 
      361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 
      421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 
      481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 
      541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 
      601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 
      661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 
      721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 
      781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 
      841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 
      901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 
      961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 
     1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 
     1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 
     1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 
     1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 
     1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 
     1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 
     1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 
     1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 
     1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 
     1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 
     1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 
     1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 
     1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 
     1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 
     1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 
     1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 
     1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 
     2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 
     2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 
     2161 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg 
     2221 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag 
     2281 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg 
     2341 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct 
     2401 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa 
     2461 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta 
     2521 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat 
     2581 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa 
     2641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 
     2701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 
     2761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 
     2821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 
     2881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 
     2941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 
     3001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 
     3061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg 
     3121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 
     3181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 
     3241 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga 
     3301 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt 
     3361 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata 
     3421 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag 
     3481 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc 
     3541 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt 
     3601 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca 
     3661 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 
     3721 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga 
     3781 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct 
     3841 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct 
     3901 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg gattctaccc 
     3961 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta 
     4021 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc 
     4081 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc 
     4141 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg 
     4201 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag 
     4261 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat 
     4321 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag 
     4381 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata 
     4441 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg 
     4501 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat 
     4561 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt 
     4621 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc 
     4681 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca 
     4741 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg 
     4801 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt 
     4861 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca 
     4921 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa 
     4981 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt 
     5041 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt 
     5101 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac 
     5161 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 
     5221 tgaggggtat cttcgaacac acgaaacttt ttccttcctt cattcacgca cactactctc 
     5281 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt 
     5341 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat 
     5401 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag 
     5461 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat 
     5521 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa 
     5581 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc 
     5641 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct 
     5701 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca 
     5761 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt 
     5821 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat 
     5881 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc 
     5941 gccggtattg caatacccag ctttgactca tatggccatg gcgggcaacc gcagcagcca 
     6001 ctcccgcttg ggaagaatag aggcagattc tgaaagtcaa gaagacatca tccggaatat 
     6061 tgccaggcac ctcgcccagg tcggggacag catggaccgt agcatccctc cgggcctggt 
     6121 gaacggcctg gccctgcagc tcaggaacac cagccggtcg gaggaggacc ggaacaggga 
     6181 cctggccact gccctggagc agctgctgca ggcctaccct agagacatgg agaaggagaa 
     6241 gaccatgctg gtgctggccc tgctgctggc caagaaggtg gccagtcaca cgccgtcctt 
     6301 gctccgtgat gtctttcaca caacagtgaa ttttattaac cagaacctac gcacctacgt 
     6361 gaggagctta gccagaaatg ggatggactg agtcgacctg cagccaagct aattccgggc 
     6421 gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata 
     6481 caaattttaa agtgactctt aggttttaaa acgaaaattc ttgttcttga gtaactcttt 
     6541 cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc gctcttattg accacacctc 
     6601 taccggcatg caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 
     6661 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 
     6721 taatgagtga ggtaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 
     6781 aacctgtcgt gccagctgga ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 
     6841 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 
     6901 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 
     6961 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 
     7021 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 
     7081 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 
     7141 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 
     7201 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 
     7261 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 
     7321 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 
     7381 gcagccacta gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 
     7441 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 
     7501 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 
     7561 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 
     7621 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 
     7681 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 
     7741 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 
     7801 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 
     7861 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 
     7921 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 
     7981 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 
     8041 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 
     8101 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 
     8161 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 
     8221 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 
     8281 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 
     8341 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 
     8401 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 
     8461 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 
     8521 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 
     8581 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 
     8641 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 
     8701 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 
     8761 tttccccgaa aagtgccacc t 
//
