LOCUS       pCAGGS-E-mBNIP3         5339 bp    DNA     circular     24-OCT-2007
DEFINITION  LMBP 4773.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4773
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 24-OCT-2007
            
COMMENT     VNTNAME|pCAGGS-E-mBNIP3|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT             "pCAGGS-E-hABIN-2" "" 0 0 1 63 1 63 1 63 0 "" "" "" "" "" "" 
COMMENT             (CObList) (CTerm 4 0 0 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 2 1 63 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 3 1 0 1098082632 0 "" 0 "" "" "" "" "" "" 
COMMENT             "pCAGGS-E-mBNIP3-seqXavier" "" 0 0 1 565 64 628 55 619 0 "" "" "" 
COMMENT             "" "" "" (CObList) (CTerm 2 0 55 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 2 1 619 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #2=(CFragment 3 1 0 11707196 0 "" 0 "" "" "" "" "" "" 
COMMENT             "pCAGGS-E-hABIN-2" "" 0 0 1 4711 629 5339 1847 6557 0 "" "" "" "" 
COMMENT             "" "" (CObList) (CTerm 2 0 1847 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 4 1 0 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 21 22 62 29 30 32 33 43) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "BamHI" "BglII" "NcoI" "KpnI" "PstI" "TaqI" "EcoRI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #4=(CRSite (CStringList) "BglII" "agatct" 2 0 1 629 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 1372 0 3988 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 63 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 93 0 1090 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "TaqI" "tcga" 2 0 8 6 0 409 0 1081 0 1897 0 
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COMMENT        #9=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 53 0 5338 0 0 "")) 
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COMMENT        #12=(CFSignal (CObList) "AG promoter" 29 0 0 3995 5336 0 (CStringList) 
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COMMENT        #13=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 3612 3989 0 
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COMMENT        #14=(CFSignal (CObList) "intron AG promoter" 15 0 0 4364 5288 0 
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COMMENT        #15=(CFSignal (CObList) "lac promoter" 30 0 1 1155 1237 0 (CStringList) 
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COMMENT        #16=(CFSignal (CObList) "SV40 ori" 33 0 0 1379 1454 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1379..1454") 
COMMENT        #17=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1485 1559 0 
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COMMENT        #18=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1485 1559 0 
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COMMENT        #19=(CFSignal (CObList) "pMB1 ori" 33 0 1 1674 2075 0 (CStringList) 
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COMMENT        #20=(CFSignal (CObList) "ampicillin" 4 0 1 2616 3476 0 (CStringList) 
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COMMENT        #21=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 721 1077 0 
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COMMENT                          "DNA Plasmid 'pCAGGS-E-mBNIP3'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5339 bp" 1) 
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COMMENT                          "#1: FRAGMENT of <a href=\"vnti:dnarna/pCAGGS-E-hABIN-2?sel=1-63\">pCAGGS-E-hABIN-2</a> ") 
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COMMENT                   "Component Fragments") 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
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COMMENT                                        "intron of the chicken \337-actin/ rabbit \337-globin hybrid promoter" 
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COMMENT                                 "intron AG promoter"))) "Intron (1 total)") 
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COMMENT                                   "{\\i Taq}I (3341)" "@N (@S)" 5 -10.848 
COMMENT                                   -8.89286 0 -10 4.28106 1.0646 #362#) 
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FEATURES             Location/Qualifiers
     misc_marker     13..51
                     /vntifkey="22"
                     /label=E-tag
                     /note="E-tag"
     CDS             64..627
                     /vntifkey="4"
                     /label=mBNIP3
                     /note="Mouse Bcl2/adenovirus E1B 19kDa interacting protein 3; coding sequence"
     promoter        3995..5336
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        3612..3989
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="human CMV-IE enhancer"
     intron          4364..5288
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="intron of the chicken ß-actin/ rabbit ß-globin hybrid promoter"
     promoter        complement(1155..1237)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     rep_origin      1379..1454
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     terminator      1485..1559
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(1485..1559)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(1674..2075)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(2616..3476)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     misc_feature    721..1077
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit ß-globin gene"
     terminator      629..720
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
BASE COUNT     1161 a      1398 c      1469 g      1311 t 
ORIGIN
        1 ttcctcgaga tgggtgcgcc ggtgccgtat ccggatccgc tggaaccgcg tgaattcggt 
       61 accatgtcgc agagcgggga ggagaacctg cagggctcct gggtagaact gcacttcagc 
      121 aatggcaatg ggagcagcgt tccagcctcc gtctctattt ataatggtga catggaaaaa 
      181 atactgttgg atgcccagca tgaatctgga cgaagtagct ccaagagttc tcactgtgac 
      241 agcccacctc gctcccagac accacaagat accaacagag ctgaaataga cagccacagc 
      301 tttggcgaga aaaacagcac tctgtctgag gaagattata ttgagagaag aagagaagtt 
      361 gaaagtatcc tgaagaaaaa ctcagattgg atatgggatt ggtcaagtcg accagaaaat 
      421 attcccccca aggagttcct ttttaaacac ccgaagcgca cagctactct cagcatgaga 
      481 aacacaagcg ttatgaagaa agggggaatt ttctcagcag actttctgaa ggttttcctt 
      541 ccatctctgt tactgtctca tctgctggcc attggcttgg ggatctacat tggaaggcgt 
      601 ctgacaactt ccactagcac cttctgaaga tctttttccc tctgccaaaa attatgggga 
      661 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc 
      721 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca 
      781 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc 
      841 tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt 
      901 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt 
      961 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt 
     1021 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct 
     1081 cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 
     1141 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctgggtgc 
     1201 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 
     1261 aaacctgtcg tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta 
     1321 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 
     1381 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 
     1441 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag 
     1501 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 
     1561 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc 
     1621 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 
     1681 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 
     1741 tcactcaaag gcggtaatac ggttatccac agaatcaggg ataacgcagg aaagaacatg 
     1801 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 
     1861 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 
     1921 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 
     1981 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 
     2041 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 
     2101 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 
     2161 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 
     2221 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 
     2281 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc 
     2341 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 
     2401 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 
     2461 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 
     2521 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 
     2581 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 
     2641 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 
     2701 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 
     2761 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 
     2821 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 
     2881 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 
     2941 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 
     3001 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 
     3061 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 
     3121 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 
     3181 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 
     3241 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 
     3301 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 
     3361 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 
     3421 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 
     3481 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 
     3541 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 
     3601 ccacctgggt cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt 
     3661 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg 
     3721 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac 
     3781 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt 
     3841 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa 
     3901 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta 
     3961 catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac 
     4021 tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt 
     4081 ttgtgcagcg atgggggcgg gggggggggg ggggcccccc ccaggcgggg cggggcgggg 
     4141 cgaggggcgg ggcggggcga ggcggaaagg tgcggcggca gccaatcaga gcggcgcgct 
     4201 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc 
     4261 gcggcgggcg ggagtcgttg cgcgctgcct tccccccgtg ccccgctccg ccgccgcctc 
     4321 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg 
     4381 cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg 
     4441 gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg 
     4501 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg 
     4561 ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag 
     4621 cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt 
     4681 gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc 
     4741 ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg 
     4801 tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc 
     4861 gggcggggcg gggccgcctc gggccgggga gggctcgggg gaaggggcgc ggcggccccc 
     4921 ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt 
     4981 gcgagagggc gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc 
     5041 gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa 
     5101 tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc 
     5161 ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct 
     5221 tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt 
     5281 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaa 
//
