LOCUS       pCAGGS-GFP              5712 bp    DNA     circular     24-OCT-2007
DEFINITION  LMBP 4800.
SOURCE      
  ORGANISM  
COMMENT     LMBP 4800
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 24-OCT-2007
            
COMMENT     VNTNAME|pCAGGS-GFP|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-GFP" 0 5712 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "BalI" "BamHI" "EcoRI" "HindII" "HindIII" "NcoI" "NheI" 
COMMENT         "XhoI") (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 
COMMENT        0 (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5712 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BalI" "tggcca" 4 0 2 195 0 973 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 3 799 0 1652 0 1989 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 3 768 0 922 0 5711 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HindII" "gtyrac" 4 0 3 513 0 780 0 3985 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 761 0 1468 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 190 0 1745 0 4361 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NheI" "gctagc" 2 0 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 752 0 0 "")) 
COMMENT       (CObList 
COMMENT        #8=(CFSignal (CObList) "GFPm" 4 0 0 24 737 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "24..737") 
COMMENT        #9=(CFSignal (CObList) "\337-globin polyA" 43 0 0 1002 1093 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1002..1093") 
COMMENT        #10=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 1094 1450 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1094..1450") 
COMMENT        #11=(CFSignal (CObList) "lac promoter" 30 0 1 1528 1610 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1528..1610)") 
COMMENT        #12=(CFSignal (CObList) "SV40 ori" 33 0 0 1752 1827 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1752..1827") 
COMMENT        #13=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1858 1932 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1858..1932") 
COMMENT        #14=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1858 1932 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(1858..1932)") 
COMMENT        #15=(CFSignal (CObList) "pMB1 ori" 33 0 1 2047 2448 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2047..2448)") 
COMMENT        #16=(CFSignal (CObList) "ampicillin" 4 0 1 2989 3849 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2989..3849)") 
COMMENT        #17=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 3985 4362 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3985..4362") 
COMMENT        #18=(CFSignal (CObList) "AG promoter" 29 0 0 4368 5709 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4368..5709") 
COMMENT        #19=(CFSignal (CObList) "intron AG promoter" 15 0 0 4737 5661 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4737..5661")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #20=(CFSignal (CObList) "LMBP 4800" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #21=(CFSignal (CObList) "Last modified: 24-OCT-2007" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #22=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #23=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pCAGGS-GFP" 2) 5 
COMMENT                   "" 0 4) 
COMMENT              #24=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pCAGGS-GFP'" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4800." 
COMMENT                          1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 10/24/07 12:47" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5712 bp" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                    (CObjectList 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #35=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
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COMMENT                     #37=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                 "" 1))) "LMBP 4800") 
COMMENT                     #39=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #40=(CLinePar (CParagraph 0 (15 #21# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 24-OCT-2007"))) 
COMMENT                   "Annotations") 
COMMENT              #41=(CFolderPar 
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COMMENT                    (CObjectList 
COMMENT                     #42=(CFolderPar 
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COMMENT                           (CObjectList 
COMMENT                            #43=(CFolderPar 
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COMMENT                                   #44=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 24    End: 737 " 1) 
COMMENT                                   #45=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "mutated A. victoria GFP coding sequence" 
COMMENT                                        1) 
COMMENT                                   #46=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   24..737" 1))) "GFPm") 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2989  End: 3849 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2989..3849)" 1))) 
COMMENT                                 "ampicillin"))) "CDS (2 total)") 
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COMMENT                           (CObjectList 
COMMENT                            #54=(CFolderPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4737  End: 5661" 1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid intron" 
COMMENT                                        1) 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4737..5661" 1))) 
COMMENT                                 "intron AG promoter"))) "Intron (1 total)") 
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COMMENT                            #60=(CFolderPar 
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COMMENT                                        "Start: 1094  End: 1450" 1) 
COMMENT                                   #62=(CLinePar 
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COMMENT                                        "3' flanking region of the rabbit \337-globin gene" 
COMMENT                                        1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #64=(CLinePar 
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COMMENT                                        "   1094..1450" 1))) 
COMMENT                                 "3' FR of \337-globin"))) 
COMMENT                          "Misc. Feature (1 total)") 
COMMENT                     #65=(CFolderPar 
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COMMENT                            #66=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3985  End: 4362" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "human CMV-IE enhancer" 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3985..4362" 1))) "hCMV-IE enhancer") 
COMMENT                            #71=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4368  End: 5709" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid promoter" 
COMMENT                                        1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4368..5709" 1))) "AG promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #76=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #77=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1528 (3 #11# 0) 1 2 2 0 194) 
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COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1528  End: 1610 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "E. coli lac operon promoter" 1) 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1528..1610)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
COMMENT                     #82=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #83=(CFolderPar 
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COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1752  End: 1827" 1) 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "bidirectional origin of SV40" 1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1752..1827" 1))) "SV40 ori") 
COMMENT                            #88=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2047 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2047  End: 2448 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "origin plasmid" 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2047..2448)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (2 total)") 
COMMENT                     #93=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                        "Start: 1002  End: 1093" 1) 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "3' UTR of the rabbit \337-globin gene, incl. polyA" 
COMMENT                                        1) 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1002..1093" 1))) "\337-globin polyA") 
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COMMENT                                         "Start: 1858  End: 1932" 1) 
COMMENT                                   #101=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "polyA signal of the SV40 early region" 
COMMENT                                         1) 
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COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Original Location Description:" 1) 
COMMENT                                   #103=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   1858..1932" 1))) "SV40 polyA early") 
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COMMENT                                          "Start: 1858  End: 1932 (Complementary)" 
COMMENT                                          1) 
COMMENT                                    #106=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "polyA signal of the SV40 late region" 
COMMENT                                          1) 
COMMENT                                    #107=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #108=(CLinePar 
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COMMENT                                          "   complement(1858..1932)" 1))) 
COMMENT                                  "SV40 polyA late"))) "Terminator (3 total)"))) 
COMMENT                   "Feature Map") 
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COMMENT                     (CObjectList 
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COMMENT                                            (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 195 " 1) 
COMMENT                                      #113=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 973 " 1))))) "BalI: 2 sites") 
COMMENT                            3 3 "TGGCCA" "ACCGGT") 
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COMMENT                                            " N1: 799 " 1) 
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COMMENT                                            " N2: 1652 " 1) 
COMMENT                                      #118=(CLinePar 
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COMMENT                                            " N3: 1989 " 1))))) "BamHI: 3 sites") 
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COMMENT                                            " N1: 768 " 1) 
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COMMENT                                            " N2: 922 " 1) 
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COMMENT                            1 5 "GAATTC" "CTTAAG") 
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COMMENT                                            " N1: 513 " 1) 
COMMENT                                      #127=(CLinePar 
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COMMENT                                            " N2: 780 " 1) 
COMMENT                                      #128=(CLinePar 
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COMMENT                                            " N3: 3985 " 1))))) 
COMMENT                             "HindII: 3 sites") 3 3 "GTYRAC" "CARYTG") 
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COMMENT                                            " N1: 761 " 1) 
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COMMENT                                            " N2: 1468 " 1))))) 
COMMENT                             "HindIII: 2 sites") 1 5 "AAGCTT" "TTCGAA") 
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COMMENT                                            " N1: 190 " 1) 
COMMENT                                      #136=(CLinePar 
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COMMENT                                            " N2: 1745 " 1) 
COMMENT                                      #137=(CLinePar 
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COMMENT                                            " N3: 4361 " 1))))) "NcoI: 3 sites") 
COMMENT                            1 5 "CCATGG" "GGTACC") 
COMMENT                      #138=(CRSFolderPar 
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COMMENT                             (CGroupPar (CParagraph 27734072 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "NheI: 0 sites") 1 5 "GCTAGC" 
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FEATURES             Location/Qualifiers
     CDS             24..737
                     /vntifkey="4"
                     /label=GFPm
                     /note="mutated A. victoria GFP coding sequence"
     terminator      1002..1093
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     misc_feature    1094..1450
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit ß-globin gene"
     promoter        complement(1528..1610)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     rep_origin      1752..1827
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     terminator      1858..1932
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(1858..1932)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(2047..2448)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(2989..3849)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     promoter        3985..4362
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="human CMV-IE enhancer"
     promoter        4368..5709
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     intron          4737..5661
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid intron"
BASE COUNT     1300 a      1462 c      1514 g      1422 t        14 others
ORIGIN
        1 ttcctcgaca tgannnnnnn nnnatgggta aaggagaaga acttttcact ggagttgtcc 
       61 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg 
      121 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac 
      181 tacctgttcc atggccaaca cttgtcacta ctttcacnta tggtgttcaa tgcttttcaa 
      241 gatacccaga tcatatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg 
      301 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca 
      361 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag 
      421 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca 
      481 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag 
      541 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg 
      601 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg 
      661 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca 
      721 tggatgaact atacaagtcc ggactcagat ctcgagctca agcttcgaat tctgcagtcg 
      781 acggtaccgc gggcccggga tccaccggat ctagataact gatcataatc agccatacca 
      841 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac 
      901 ataaaatgaa tgnnntcgag gaattcactc ctcaggtgca ggctgcctat cagaaggtgg 
      961 tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca 
     1021 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat 
     1081 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg 
     1141 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg 
     1201 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac 
     1261 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt 
     1321 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt 
     1381 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct 
     1441 tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct 
     1501 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 
     1561 aaagcctggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 
     1621 ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat 
     1681 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 
     1741 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 
     1801 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac 
     1861 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 
     1921 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 
     1981 catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 
     2041 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 
     2101 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca gggataacgc 
     2161 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 
     2221 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 
     2281 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 
     2341 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 
     2401 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 
     2461 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 
     2521 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 
     2581 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 
     2641 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 
     2701 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 
     2761 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 
     2821 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 
     2881 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 
     2941 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 
     3001 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 
     3061 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 
     3121 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 
     3181 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 
     3241 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 
     3301 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 
     3361 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 
     3421 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 
     3481 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 
     3541 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 
     3601 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 
     3661 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 
     3721 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 
     3781 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 
     3841 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 
     3901 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 
     3961 tccccgaaaa gtgccacctg ggtcgacatt gattattgac tagttattaa tagtaatcaa 
     4021 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 
     4081 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     4141 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     4201 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     4261 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 
     4321 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca 
     4381 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta 
     4441 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggccc cccccaggcg 
     4501 gggcggggcg gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc 
     4561 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata 
     4621 aaaagcgaag cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct 
     4681 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga 
     4741 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg 
     4801 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg 
     4861 ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg 
     4921 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg 
     4981 tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga 
     5041 acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg 
     5101 tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg 
     5161 gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc 
     5221 aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaagggg 
     5281 cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt 
     5341 ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg 
     5401 aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc 
     5461 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc 
     5521 cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg 
     5581 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat 
     5641 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat 
     5701 tttggcaaag aa 
//
