ID pCAGGS-GFP standard; circular DNA; ; 5712 BP. DE LMBP 4800. CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium). CC Last modified: 24-OCT-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS 24..737 FT /vntifkey="4" FT /label=GFPm FT /note="mutated A. victoria GFP coding sequence" FT terminator 1002..1093 FT /vntifkey="43" FT /label=ß-globin\polyA FT /note="3' UTR of the rabbit ß-globin gene, incl. polyA" FT misc_feature 1094..1450 FT /vntifkey="21" FT /label=3'\FR\of\ß-globin FT /note="3' flanking region of the rabbit ß-globin gene" FT promoter complement(1528..1610) FT /vntifkey="30" FT /label=lac\promoter FT /note="E. coli lac operon promoter" FT rep_origin 1752..1827 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT terminator 1858..1932 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(1858..1932) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT rep_origin complement(2047..2448) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(2989..3849) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" FT promoter 3985..4362 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT /note="human CMV-IE enhancer" FT promoter 4368..5709 FT /vntifkey="29" FT /label=AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid promoter" FT intron 4737..5661 FT /vntifkey="15" FT /label=intron\AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid intron" SQ Sequence 5712 BP; 1300 A; 1462 C; 1514 G; 1422 t; 14 other; ttcctcgaca tgannnnnnn nnnatgggta aaggagaaga acttttcact ggagttgtcc 60 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg 120 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac 180 tacctgttcc atggccaaca cttgtcacta ctttcacnta tggtgttcaa tgcttttcaa 240 gatacccaga tcatatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg 300 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca 360 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag 420 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca 480 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag 540 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg 600 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg 660 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca 720 tggatgaact atacaagtcc ggactcagat ctcgagctca agcttcgaat tctgcagtcg 780 acggtaccgc gggcccggga tccaccggat ctagataact gatcataatc agccatacca 840 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac 900 ataaaatgaa tgnnntcgag gaattcactc ctcaggtgca ggctgcctat cagaaggtgg 960 tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca 1020 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat 1080 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg 1140 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg 1200 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac 1260 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt 1320 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt 1380 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct 1440 tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct 1500 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 1560 aaagcctggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 1620 ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat 1680 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 1740 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 1800 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac 1860 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 1920 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 1980 catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 2040 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 2100 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca gggataacgc 2160 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 2220 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 2280 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 2340 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 2400 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 2460 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 2520 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 2580 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 2640 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 2700 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 2760 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 2820 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 2880 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 2940 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 3000 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 3060 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 3120 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 3180 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 3240 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 3300 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 3360 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 3420 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 3480 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 3540 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 3600 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 3660 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 3720 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 3780 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 3840 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 3900 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 3960 tccccgaaaa gtgccacctg ggtcgacatt gattattgac tagttattaa tagtaatcaa 4020 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 4080 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 4140 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 4200 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 4260 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 4320 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca 4380 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta 4440 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggccc cccccaggcg 4500 gggcggggcg gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc 4560 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata 4620 aaaagcgaag cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct 4680 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga 4740 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg 4800 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg 4860 ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg 4920 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg 4980 tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga 5040 acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg 5100 tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg 5160 gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc 5220 aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaagggg 5280 cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt 5340 ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg 5400 aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc 5460 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc 5520 cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg 5580 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat 5640 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat 5700 tttggcaaag aa 5712 //