ID pCAGGS-FLAGAVPShOMI-S306A standard; circular DNA; ; 5747 BP. DE LMBP 4843. CC Accession number: LMBP 4843 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 28-JAN-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS 43..1023 FT /vntifkey="4" FT /label=mature\hOMI\mutant FT misc_marker 13..36 FT /vntifkey="22" FT /label=FLAG\epitope FT terminator 1033..1124 FT /vntifkey="43" FT /label=ß-globin\polyA FT misc_feature 1125..1481 FT /vntifkey="21" FT /label=3'\FR\of\ß-globin FT /note="3' flanking region of the rabbit ß-globin gene" FT promoter complement(1559..1641) FT /vntifkey="30" FT /label=lac\promoter FT rep_origin 1783..1858 FT /vntifkey="33" FT /label=SV40\ori FT terminator 1889..1963 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(1889..1963) FT /vntifkey="43" FT /label=SV40\polyA\late FT rep_origin complement(2078..2479) FT /vntifkey="33" FT /label=pMB1\ori FT promoter 4020..4397 FT /vntifkey="29" FT /label=hCMV\enhancer FT promoter 4403..5744 FT /vntifkey="29" FT /label=AG\promoter FT intron 4772..5696 FT /vntifkey="15" FT /label=intron\AG\promoter FT CDS complement(3020..3880) FT /vntifkey="4" FT /label=ampicillin FT mutation 562..567 FT /vntifkey="62" FT /label=S306A FT /note="WT: TCT.GGA" SQ Sequence 5747 BP; 1209 A; 1530 C; 1607 G; 1401 t; ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggccgt ccctagcccg 60 ccgcccgctt ctccccggag tcagtacaac ttcatcgcag atgtggtgga gaagacagca 120 cctgccgtgg tctatatcga gatcctggac cggcaccctt tcttgggccg cgaggtccct 180 atctcgaacg gctcaggatt cgtggtggct gccgatgggc tcattgtcac caacgcccat 240 gtggtggctg atcggcgcag agtccgtgtg agactgctaa gcggcgacac gtatgaggcc 300 gtggtcacag ctgtggatcc cgtggcagac atcgcaacgc tgaggattca gactaaggag 360 cctctcccca cgctgcctct gggacgctca gctgatgtcc ggcaagggga gtttgttgtt 420 gccatgggaa gtccctttgc actgcagaac acgatcacat ccggcattgt tagctctgct 480 cagcgtccag ccagagacct gggactcccc caaaccaatg tggaatacat tcaaactgat 540 gcagctattg attttggaaa cgccggcggt cccctggtta acctggatgg ggaggtgatt 600 ggagtgaaca ccatgaaggt cacagctgga atctcctttg ccatcccttc tgatcgtctt 660 cgagagtttc tgcatcgtgg ggaaaagaag aattcctcct ccggaatcag tgggtcccag 720 cggcgctaca ttggggtgat gatgctgacc ctgagtccca gcatccttgc tgaactacag 780 cttcgagaac caagctttcc cgatgttcag catggtgtac tcatccataa agtcatcctg 840 ggctcccctg cacaccgggc tggtctgcgg cctggtgatg tgattttggc cattggggag 900 cagatggtac aaaatgctga agatgtttat gaagctgttc gaacccaatc ccagttggca 960 gtgcagatcc ggcggggacg agaaacactg accttatatg tgacccctga ggtcacagaa 1020 tgagtcgacg aagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt 1080 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat 1140 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat 1200 gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt 1260 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat 1320 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct 1380 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga 1440 ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag 1500 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 1560 acacaacata cgagccggaa gcataaagtg taaagcctgg gtgcctaatg agtgagctaa 1620 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 1680 cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 1740 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 1800 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 1860 ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa 1920 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 1980 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc 2040 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 2100 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 2160 atacggttat ccacagaatc agggataacg caggaaagaa catgtgagca aaaggccagc 2220 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 2280 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 2340 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 2400 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 2460 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 2520 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 2580 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 2640 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 2700 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 2760 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 2820 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 2880 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 2940 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 3000 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 3060 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 3120 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 3180 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 3240 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 3300 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 3360 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 3420 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 3480 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 3540 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 3600 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 3660 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 3720 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 3780 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 3840 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 3900 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 3960 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcgatcg 4020 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc 4080 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa 4140 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 4200 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 4260 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 4320 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt 4380 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc 4440 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat 4500 gggggcgggg gggggggggg ggcccccccc aggcggggcg gggcggggcg aggggcgggg 4560 cggggcgagg cggaaaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc 4620 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg 4680 agtcgttgcg cgctgccttc cccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc 4740 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 4800 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag 4860 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt 4920 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg 4980 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg 5040 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg 5100 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc 5160 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg 5220 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg 5280 gccgcctcgg gccggggagg gctcggggga aggggcgcgg cggcccccgg agcgccggcg 5340 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc 5400 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc 5460 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg 5520 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc 5580 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga 5640 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc 5700 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaa 5747 //