ID pCAGGS-FLAGmA20d5 standard; circular DNA; ; 6640 BP. DE LMBP 4844. CC Accession number: LMBP 4844 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 17-AUG-2009 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_marker 13..36 FT /vntifkey="22" FT /label=FLAG\epitope FT promoter complement(2452..2534) FT /vntifkey="30" FT /label=lac\promoter FT terminator 1926..2017 FT /vntifkey="43" FT /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA FT promoter 4913..5290 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT CDS complement(3913..4773) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin 2676..2751 FT /vntifkey="33" FT /label=SV40\ori FT rep_origin complement(2971..3372) FT /vntifkey="33" FT /label=pMB1\ori FT intron 5665..6589 FT /vntifkey="15" FT /label=intron\AG\promoter FT CDS 46..1914 FT /vntifkey="4" FT /label=mA20\(d547-699) FT misc_feature 2018..2374 FT /vntifkey="21" FT /label=3'\flanking\region\of\rabbit\ß-globin FT terminator 2782..2856 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(2782..2856) FT /vntifkey="43" FT /label=SV40\polyA\late FT promoter 5296..6637 FT /vntifkey="29" FT /label=AG\promoter SQ Sequence 6640 BP; 1511 A; 1752 C; 1780 G; 1597 t; ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatggc tgaacaactt 60 cttcctcagg ctttgtattt gagcaatatg cggaaagctg tgaagatacg agagagaacc 120 ccagaagaca ttttcaaacc taccaatggg atcatctatc actttaaaac catgcaccga 180 tacacgctgg agatgttcag aacatgccag ttttgcccac agttccgaga gatcatccac 240 aaagcactta ttgacagaag tgtccaggct tccctggaaa gccagaagaa gctcaactgg 300 tgtcgtgaag tcaggaagct cgtggctctg aaaaccaatg gtgatggaaa ctgcctcatg 360 catgcagctt gtcagtacat gtggggtgtt caggatactg acctggtcct gaggaaggcc 420 ctctgcagca cccttaagga gacagacact cggaacttta aattccgctg gcagctggaa 480 tctctgaaat ctcaggaatt tgtggaaaca ggactttgct acgacactcg gaactggaat 540 gacgaatggg acaacttggt caaaatggca tcagcagaca cacctgcagc ccgaagtgga 600 cttcagtaca attccctgga agaaatccac atatttgtcc tcagcaacat cctcagaaga 660 cccatcattg tcatttcaga caaaatgcta agaagtttgg aatctggttc caattttgct 720 cctttgaaag tgggtgggat ttatctgcct cttcactggc ctgcccagga gtgttacaga 780 tatcccatcg tcctaggcta tgacagccag cactttgtac ccctggtgac cctgaaggac 840 agtggacctg aacttcgcgc tgttccactt gttaacagag accggggtag gtttgaagac 900 ttaaaagttc acttcttgac agatcctgag aatgagatga aggaaaagct tctaaaggag 960 tacttgatag tgatggagat ccctgtgcaa ggctgggacc acggcacgac tcacctgatc 1020 aacgctgcaa aattggatga agctaactta cccaaagaaa taaatttggt agacgattac 1080 tttgagcttg ttcagcacga atacaagaaa tggcaggaga acagcgatca ggccaggaga 1140 gcggcacatg cgcagaaccc cttggagcct tccacacccc agctatcact catggatata 1200 aaatgtgaga cacccaactg tcctttcttc atgtccgtga acactcagcc tttatgccac 1260 gaatgctcag agaggcgcca aaagaatcag agcaagctcc caaagctgaa ctcgaagcta 1320 ggccctgaag gactcccagg cgtgggactt ggctcctcaa actggagccc cgaggaaacc 1380 gctggaggac ctcattcagc cccacccaca gcacccagcc tttttctctt cagtgagacc 1440 actgcaatga agtgcaggag tcctgggtgc ccttttactt tgaatgtgca gcataatgga 1500 ttctgtgagc gttgccacgc ccggcagatt aatgccagcc acaccgcaga ccctggaaag 1560 tgccaagcct gccttcagga tgtcactcgg acctttaatg gcatctgcag tacctgtttc 1620 aaaaggacta cagcagagcc cagctccagc ctcacttcca gtatccctgc ctcctgtcac 1680 caaaaatgtg cccgggcctc ctgcaagaac attctggcct gtcgcagtga ggaactctgt 1740 atggagtgcc agcacctaag ccaacgagta ggttctgtgg cccaccgggg tgagcccacg 1800 cctgaagagc cccctaaaca gcgctgccgg gcccctgctt gtgatcactt tggcaatgcc 1860 aagtgtaatg gttactgcaa tgagtgctac cagttcaagc agatgtatgg ctaagtgtcg 1920 acgaagatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc 1980 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt 2040 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt 2100 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat 2160 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag 2220 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat 2280 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc 2340 cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg 2400 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 2460 atacgagccg gaagcataaa gtgtaaagcc tgggtgccta atgagtgagc taactcacat 2520 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc 2580 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac 2640 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 2700 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 2760 cctaggcttt tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc 2820 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 2880 tccaaactca tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac 2940 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 3000 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 3060 tatccacaga atcagggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 3120 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 3180 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 3240 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 3300 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 3360 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 3420 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 3480 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 3540 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 3600 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 3660 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 3720 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 3780 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 3840 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 3900 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 3960 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 4020 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 4080 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 4140 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 4200 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 4260 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 4320 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 4380 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 4440 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 4500 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 4560 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 4620 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 4680 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 4740 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 4800 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 4860 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga tcgacattga 4920 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg 4980 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 5040 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 5100 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 5160 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 5220 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 5280 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc 5340 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg 5400 gggggggggg gggggccccc cccaggcggg gcggggcggg gcgaggggcg gggcggggcg 5460 aggcggaaag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg 5520 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgtt 5580 gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc 5640 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt 5700 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag 5760 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg 5820 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc 5880 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc 5940 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg 6000 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc 6060 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg 6120 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct 6180 cgggccgggg agggctcggg ggaaggggcg cggcggcccc cggagcgccg gcggctgtcg 6240 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact 6300 tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag 6360 cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg 6420 tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga 6480 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg 6540 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa 6600 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa 6640 //