ID pCAGGS-E-SptP standard; circular DNA; ; 6521 BP. DE LMBP 4878. CC Accession number: LMBP 4878 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 2-DEC-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_marker 16..54 FT /vntifkey="22" FT /label=E-tag FT terminator 1811..1902 FT /vntifkey="43" FT /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA FT misc_feature 1903..2259 FT /vntifkey="21" FT /label=3'\flanking\region\of\rabbit\ß-globin FT promoter complement(2337..2419) FT /vntifkey="30" FT /label=lac\promoter FT rep_origin 2561..2636 FT /vntifkey="33" FT /label=SV40\ori FT terminator 2667..2741 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(2667..2741) FT /vntifkey="43" FT /label=SV40\polyA\late FT rep_origin complement(2856..3257) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(3798..4658) FT /vntifkey="4" FT /label=ampicillin\resistance FT promoter 4794..5171 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT promoter 5177..6518 FT /vntifkey="29" FT /label=AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid promoter" FT intron 5546..6470 FT /vntifkey="15" FT /label=intron\AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid promoter intron" FT CDS 64..1695 FT /vntifkey="4" FT /label=sptP SQ Sequence 6521 BP; 1557 A; 1619 C; 1770 G; 1575 t; ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 60 gctatgctaa agtatgagga gagaaaattg aataatttaa cgttgtcttc gttttcaaaa 120 gttggtgtgt cgaatgatgc ccgactttat attgctaagg aaaatactga taaggcatat 180 gttgcgcctg aaaaattttc gtcaaaagta ttaacctggc ttggaaaaat gccgttattt 240 aaaaacactg aagtggtgca aaaacatacg gaaaatatca gagtacagga ccaaaagatt 300 ttacagacat ttctccatgc actaacggaa aaatatgggg aaacagcggt taatgacgca 360 ctgttaatgt cccgtataaa tatgaacaaa ccccttaccc aacgtttagc agtgcagatc 420 acggagtgtg taaaagctgc tgacgaaggg tttataaacc ttattaagag caaggataat 480 gttggtgtca ggaatgccgc tttagtcata aaaggcggcg atacaaaagt ggcagaaaaa 540 aataacgatg ttggagcaga aagtaagcaa cctttactcg atatcgcgct aaagggactt 600 aaaagaacat taccgcaact ggaacaaatg gatggaaata gcctgcgaga aaacttccag 660 gagatggctt caggtaacgg cccgctgcgt tcattaatga cgaatttaca gaatttaaat 720 aaaattccag aggctaaaca acttaatgat tatgttacaa cgttaacaaa tattcaggtc 780 ggtgtagccc ggttctcaca atggggaacc tgtggaggag aggttgagag gtgggttgat 840 aaagcctcta ctcatgaatt aacccaggct gtaaaaaaaa ttcacgttat tgctaaagaa 900 ctgaaaaatg ttacagctga acttgagaaa atagaagccg gcgcgccaat gccacagacg 960 atgagcggac cgacactggg cctggcacga tttgcggtca gcagcattcc aattaatcag 1020 caaacccagg tgaaactgag cgatggaatg cctgtgccag tgaatacgtt aacttttgac 1080 ggtaagcctg tggcattagc cggttcgtac ccaaaaaata cgccggatgc gctggaagcg 1140 catatgaaaa tgcttcttga aaaagaatgc tcgtgcctgg tggtgttaac gtcggaagat 1200 cagatgcagg caaaacaatt accaccctat ttcagaggga gctacacctt tggcgaggtg 1260 cataccaaca gccaaaaagt gagctcagcc agtcagggag aagcgataga ccaatacaat 1320 atgcaactgt cctgcgggga aaagcggtat acaatcccgg tattgcatgt gaaaaattgg 1380 ccagatcacc agccgttacc gtctacggat cagttagaat acctggcgga tagggtgaaa 1440 aatagtaacc aaaatggcgc gccggggcgt agttcatcag ataagcattt accgatgatt 1500 cattgtctgg gcggagtggg aagaaccgga acgatggcgg cggcccttgt acttaaggat 1560 aatcctcata gtaatctgga gcaggtacgt gcagatttcc gggattcacg gaacaataga 1620 atgctggaag acgcctcaca gtttgtacaa ctaaaagcaa tgcaagccca gttgcttatg 1680 acgacggcaa gctgacccgg gacattaaac gcgtgggtac cggatccggc aattcactcc 1740 tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa 1800 ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga 1860 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt 1920 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga 1980 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg 2040 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag 2100 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt 2160 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact 2220 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct 2280 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 2340 acaacatacg agccggaagc ataaagtgta aagcctgggt gcctaatgag tgagctaact 2400 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg 2460 gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc 2520 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 2580 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 2640 ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat 2700 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 2760 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg 2820 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 2880 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 2940 acggttatcc acagaatcag ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 3000 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 3060 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 3120 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 3180 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 3240 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 3300 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 3360 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 3420 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 3480 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 3540 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 3600 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 3660 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 3720 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 3780 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 3840 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 3900 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 3960 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 4020 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 4080 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 4140 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 4200 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 4260 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 4320 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 4380 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 4440 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 4500 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 4560 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 4620 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 4680 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 4740 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgacattg 4800 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat 4860 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc 4920 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca 4980 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta 5040 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta 5100 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat 5160 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc 5220 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc 5280 gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc ggggcggggc 5340 gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat 5400 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgt 5460 tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct 5520 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg 5580 taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga 5640 ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt 5700 gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg 5760 cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc 5820 cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg 5880 ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc 5940 ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg 6000 gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc 6060 tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc ggcggctgtc 6120 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac 6180 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta 6240 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc 6300 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg 6360 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg 6420 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca 6480 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga a 6521 //