ID pACCMVpLpA-E-hRac1-CA standard; circular DNA; ; 9447 BP. DE LMBP 4885. CC Accession number: LMBP 4885 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 3-AUG-2009 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS 1348..1923 FT /vntifkey="4" FT /label=hRAC1-CA FT promoter 673..1256 FT /vntifkey="29" FT /label=hCMV-IE\promoter\and\enhancer FT rep_origin complement(7429..7831) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(8372..9232) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin 1..454 FT /vntifkey="33" FT /label=Ad5\ori FT /note="Ad5 inverted terminal repeat (ITR) incl. ori and packaging signals" FT terminator 2276..2372 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(2276..2372) FT /vntifkey="43" FT /label=SV40\polyA\late FT misc_marker 1300..1338 FT /vntifkey="22" FT /label=E-tag FT CDS 1997..2071 FT /vntifkey="4" FT /label=part\of\SV40\small\t-antigen FT intron 2072..2138 FT /vntifkey="15" FT /label=intron\of\SV40\small\t-antigen FT misc_feature 2446..5348 FT /vntifkey="21" FT /label=Ad5\flanking\np3334-6236 FT /note="homologous recombination target sequence" FT mutation 1378..1380 FT /vntifkey="62" FT /label=G12V FT /note="WT: GGA" SQ Sequence 9447 BP; 2128 A; 2376 C; 2572 G; 2371 t; catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420 cgggtcaaag ttggcgtttt attattatag tcagtctagg cggccgcgat ctatacattg 480 aatcaatatt ggcaattagc catattagtc attggttata tagcataaat caatattggc 540 tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt 600 ccaatatgac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg 660 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc 720 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc 780 atagtaacgc caatagggac tttccattga cgtcaataat gacgtatgtt cccatagtaa 840 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact 900 tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta 960 aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt 1020 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg 1080 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg 1140 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc 1200 cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt 1260 tagtgaaccg tcagatccgg tcgcgcgaat tccaccatgg gtgcgccggt gccgtatccg 1320 gacccgctgg aaccgcgtgc ggccgctcag gccatcaagt gtgtggtggt gggagacgta 1380 gctgtaggta aaacttgcct actgatcagt tacacaacca atgcatttcc tggagaatat 1440 atccctactg tctttgacaa ttattctgcc aatgttatgg tagatggaaa accggtgaat 1500 ctgggcttat gggatacagc tggacaagaa gattatgaca gattacgccc cctatcctat 1560 ccgcaaacag atgtgttctt aatttgcttt tcccttgtga gtcctgcatc atttgaaaat 1620 gtccgtgcaa agtggtatcc tgaggtgcgg caccactgtc ccaacactcc catcatccta 1680 gtgggaacta aacttgatct tagggatgat aaagacacga tcgagaaact gaaggagaag 1740 aagctgactc ccatcaccta tccgcagggt ctagccatgg ctaaggagat tggtgctgta 1800 aaatacctgg agtgctcggc gctcacacag cgaggcctca agacagtgtt tgacgaagcg 1860 atccgagcag tcctctgccc gcctcccgtg aagaagagga agagaaaatg cctgctgttg 1920 tgactcgagc ccgggacatt aaacgcgtgg gtacccgggg atcctctaga gtcgacctgc 1980 aggcatgcaa gcttgggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg 2040 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat 2100 gtgttaaact actgattcta attgtttgtg tattttagat tcacagtccc aaggctcatt 2160 tcaggcccct cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag 2220 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga 2280 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata 2340 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 2400 aactcatcaa tgtatcttat catgtctgga tcgcggccgc ctagaggaag gtgctgaggt 2460 acgatgagac ccgcaccagg tgcagaccct gcgagtgtgg cggtaaacat attaggaacc 2520 agcctgtgat gctggatgtg accgaggagc tgaggcccga tcacttggtg ctggcctgca 2580 cccgcgctga gtttggctct agcgatgaag atacagattg aggtactgaa atgtgtgggc 2640 gtggcttaag ggtgggaaag aatatataag gtgggggtct tatgtagttt tgtatctgtt 2700 ttgcagcagc cgccgccgcc atgagcacca actcgtttga tggaagcatt gtgagctcat 2760 atttgacaac gcgcatgccc ccatgggccg gggtgcgtca gaatgtgatg ggctccagca 2820 ttgatggtcg ccccgtcctg cccgcaaact ctactacctt gacctacgag accgtgtctg 2880 gaacgccgtt ggagactgca gcctccgccg ccgcttcagc cgctgcagcc accgcccgcg 2940 ggattgtgac tgactttgct ttcctgagcc cgcttgcaag cagtgcagct tcccgttcat 3000 ccgcccgcga tgacaagttg acggctcttt tggcacaatt ggattctttg acccgggaac 3060 ttaatgtcgt ttctcagcag ctgttggatc tgcgccagca ggtttctgcc ctgaaggctt 3120 cctcccctcc caatgcggtt taaaacataa ataaaaaacc agactctgtt tggatttgga 3180 tcaagcaagt gtcttgctgt ctttatttag gggttttgcg cgcgcggtag gcccgggacc 3240 agcggtctcg gtcgttgagg gtcctgtgta ttttttccag gacgtggtaa aggtgactct 3300 ggatgttcag atacatgggc ataagcccgt ctctggggtg gaggtagcac cactgcagag 3360 cttcatgctg cggggtggtg ttgtagatga tccagtcgta gcaggagcgc tgggcgtggt 3420 gcctaaaaat gtctttcagt agcaagctga ttgccagggg caggcccttg gtgtaagtgt 3480 ttacaaagcg gttaagctgg gatgggtgca tacgtgggga tatgagatgc atcttggact 3540 gtatttttag gttggctatg ttcccagcca tatccctccg gggattcatg ttgtgcagaa 3600 ccaccagcac agtgtatccg gtgcacttgg gaaatttgtc atgtagctta gaaggaaatg 3660 cgtggaagaa cttggagacg cccttgtgac ctccaagatt ttccatgcat tcgtccataa 3720 tgatggcaat gggcccacgg gcggcggcct gggcgaagat atttctggga tcactaacgt 3780 catagttgtg ttccaggatg agatcgtcat aggccatttt tacaaagcgc gggcggaggg 3840 tgccagactg cggtataatg gttccatccg gcccaggggc gtagttaccc tcacagattt 3900 gcatttccca cgctttgagt tcagatgggg ggatcatgtc tacctgcggg gcgatgaaga 3960 aaacggtttc cggggtaggg gagatcagct gggaagaaag caggttcctg agcagctgcg 4020 acttaccgca gccggtgggc ccgtaaatca cacctattac cgggtgcaac tggtagttaa 4080 gagagctgca gctgccgtca tccctgagca ggggggccac ttcgttaagc atgtccctga 4140 ctcgcatgtt ttccctgacc aaatccgcca gaaggcgctc gccgcccagc gatagcagtt 4200 cttgcaagga agcaaagttt ttcaacggtt tgagaccgtc cgccgtaggc atgcttttga 4260 gcgtttgacc aagcagttcc aggcggtccc acagctcggt cacctgctct acggcatctc 4320 gatccagcat atctcctcgt ttcgcgggtt ggggcggctt tcgctgtacg gcagtagtcg 4380 gtgctcgtcc agacgggcca gggtcatgtc tttccacggg cgcagggtcc tcgtcagcgt 4440 agtctgggtc acggtgaagg ggtgcgctcc gggctgcgcg ctggccaggg tgcgcttgag 4500 gctggtcctg ctggtgctga agcgctgccg gtcttcgccc tgcgcgtcgg ccaggtagca 4560 tttgaccatg gtgtcatagt ccagcccctc cgcggcgtgg cccttggcgc gcagcttgcc 4620 cttggaggag gcgccgcacg aggggcagtg cagacttttg agggcgtaga gcttgggcgc 4680 gagaaatacc gattccgggg agtaggcatc cgcgccgcag gccccgcaga cggtctcgca 4740 ttccacgagc caggtgagct ctggccgttc ggggtcaaaa accaggtttc ccccatgctt 4800 tttgatgcgt ttcttacctc tggtttccat gagccggtgt ccacgctcgg tgacgaaaag 4860 gctgtccgtg tccccgtata cagacttgag aggcctgtcc tcgagcggtg ttccgcggtc 4920 ctcctcgtat agaaactcgg accactctga gacaaaggct cgcgtccagg ccagcacgaa 4980 ggaggctaag tgggaggggt agcggtcgtt gtccactagg gggtccactc gctccagggt 5040 gtgaagacac atgtcgccct cttcggcatc aaggaaggtg attggtttgt aggtgtaggc 5100 cacgtgaccg ggtgttcctg aaggggggct ataaaagggg gtgggggcgc gttcgtcctc 5160 actctcttcc gcatcgctgt ctgcgagggc cagctgttgg ggtgagtact ccctctgaaa 5220 agcgggcatg acttctgcgc taagattgtc agtttccaaa aacgaggagg atttgatatt 5280 cacctggccc gcggtgatgc ctttgagggt ggccgcatcc atctggtcag aaaagacaat 5340 ctttttgttg tcaagctagc ttctatgggt ttaacaatac ctgtgtggaa gcctggaccg 5400 atgtaagggt tcggggctgt gccttttact gctgctggaa gggggtggtg tgtcgcccca 5460 aaagcagggc ttcaattaag aaatgcctct ttgaaaggtg taccttgggt atcctgtctg 5520 agggtaactc cagggtgcgc cacaatgtgg cctccgactg tggttgcttc atgctagtga 5580 aaagcgtggc tgtgattaag cataacatgg tatgtggcaa ctgcgaggac agggcctctc 5640 agatgctgac ctgctcggac ggcaactgtc acctgctgaa gaccattcac gtagccagcc 5700 actctcgcaa ggcctggcca gtgtttgagc ataacatact gacccgctgt tccttgcatt 5760 tgggtaacag gaggggggtg ttcctacctt accaatgcaa tttgagtcac actaagatat 5820 tgcttgagcc cgagagcatg tccaaggtga acctgaacgg ggtgtttgac atgaccatga 5880 agatctgcgt ctccggtctc gccgcttaga tcgctctgtg tagtagttgt agtatatcca 5940 ctctctcaaa gcatccaggc gccccctggc ttcgggttct atgtaaactc cttcatgcgc 6000 cgctgccctg ataacatcca ccaccgcaga ataagccaca cccagccaac ctacacattc 6060 gttctgcgag tcacacacgg gaggagcggg aagagctgga agaaccatgt tttttttttt 6120 attccaaaag attatccaaa acctcaaaat gaagatcctc tacgccggac gcatcgtggc 6180 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg 6240 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc 6300 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc 6360 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa 6420 gggagagcgt cgatcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt 6480 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg 6540 taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg 6600 cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct 6660 tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg 6720 cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc 6780 ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt 6840 ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca 6900 gcctaacttc gatcactgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca 6960 catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt 7020 tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc 7080 taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc 7140 gcaaaccaac ccttggcaga acatatccat cgcgtccgcc atctccagca gccgcacgcg 7200 gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt 7260 gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat caccgatacg 7320 cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat 7380 ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc tcttccgctt 7440 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 7500 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 7560 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 7620 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 7680 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 7740 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 7800 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 7860 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 7920 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 7980 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 8040 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 8100 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 8160 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 8220 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 8280 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 8340 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 8400 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 8460 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 8520 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 8580 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 8640 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg 8700 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 8760 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 8820 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 8880 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 8940 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata 9000 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 9060 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 9120 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 9180 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 9240 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 9300 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 9360 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga 9420 ggccctttcg tcttcaagaa ttaattc 9447 //