LOCUS       pACCMVpLpA-E-CrmA 9888 bp dsDNACircular
DEFINITION  LMBP 4887.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     CDS             10..1080
                     /note="ORF: Frame #1   Start: atg (4855)   Stop:  taa (5925)"
                     /label=E-tag-CrmA
     promoter        9068..9878
                     /note="CMV immediate early promoter"
                     /label=hCMV\promoter
     misc_feature    1600..4511
                     /note="homologous recombination target sequence"
                     /label=Ad5\flanking\np3334-6236
     rep_origin      6583..6985
                     /note="pBR322 origin of replication"
                     /label=pMB1\ori
     CDS             complement(7526..8386)
                     /note="ampicillin resistance gene"
                     /label=AMP
     rep_origin      8602..9055
                     /note="Ad5 ITR including origin of replication and packaging signals"
                     /label=Ad5\ori
     terminator      1292..1526
                     /note="SV40 splice/polyA signal"
                     /label=SV40\splice/polyA
ORIGIN
        1 aattccacca tgggtgcgcc ggtgccgtat ccggacccgc tggaaccgcg tgcggccgct 
       61 gatatcttca gggaaatcgc atcttctatg aaaggagaga atgtattcat ttctccaccg 
      121 tcaatctcgt cagtattgac aatactgtat tatggagcta atggatccac tgctgaacag 
      181 ctatcaaaat atgtagaaaa ggaggcggac aagaataagg atgatatctc attcaagtcc 
      241 atgaataaag tatatgggcg atattctgca gtgtttaaag attccttttt gagaaaaatt 
      301 ggagataatt tccaaactgt tgacttcact gattgtcgca ctgtagatgc gatcaacaag 
      361 tgtgttgata tcttcactga ggggaaaatt aatccactat tggatgaacc attgtctcca 
      421 gatacctgtc tcctagcaat tagtgccgta tactttaaag caaaatggtt gatgccattt 
      481 gaaaaggaat ttaccagtga ttatcccttt tacgtatctc caacggaaat ggtagatgta 
      541 agtatgatgt ctatgtacgg cgaggcattt aatcacgcat ctgtaaaaga atcattcggc 
      601 aacttttcaa tcatagaact gccatatgtt ggagatacta gtatggtggt aattcttcca 
      661 gacaatattg atggactaga atccatagaa caaaatctaa cagatacaaa ttttaagaaa 
      721 tggtgtgact ctatggatgc tatgtttatc gatgtgcaca ttcccaagtt taaggtaaca 
      781 ggctcgtata atctggtgga tgcgctagta aagttgggac tgacagaggt gttcggttca 
      841 actggagatt atagcaatat gtgtaattca gatgtgagtg tcgacgctat gatccacaaa 
      901 acgtatatag atgtcaatga agagtataca gaagcagctg cagcaacttg tgcgctggtg 
      961 gcagactgtg catcaacagt tacaaatgag ttctgtgcag atcatccgtt catctatgtg 
     1021 attaggcatg tcgatggcaa aattcttttc gttggtagat attgctctcc aacaactaat 
     1081 taacccggga cattaaacgc gtgggtaccc ggggatcctc tagagtcgac ctgcaggcat 
     1141 gcaagcttgg gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac 
     1201 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta 
     1261 aactactgat tctaattgtt tgtgtatttt agattcacag tcccaaggct catttcaggc 
     1321 ccctcagtcc tcacagtctg ttcatgatca taatcagcca taccacattt gtagaggttt 
     1381 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa 
     1441 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 
     1501 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 
     1561 tcaatgtatc ttatcatgtc tggatcgcgg ccgcctagag gaaggtgctg aggtacgatg 
     1621 agacccgcac caggtgcaga ccctgcgagt gtggcggtaa acatattagg aaccagcctg 
     1681 tgatgctgga tgtgaccgag gagctgaggc ccgatcactt ggtgctggcc tgcacccgcg 
     1741 ctgagtttgg ctctagcgat gaagatacag attgaggtac tgaaatgtgt gggcgtggct 
     1801 taagggtggg aaagaatata taaggtgggg gtcttatgta gttttgtatc tgttttgcag 
     1861 cagccgccgc cgccatgagc accaactcgt ttgatggaag cattgtgagc tcatatttga 
     1921 caacgcgcat gcccccatgg gccggggtgc gtcagaatgt gatgggctcc agcattgatg 
     1981 gtcgccccgt cctgcccgca aactctacta ccttgaccta cgagaccgtg tctggaacgc 
     2041 cgttggagac tgcagcctcc gccgccgctt cagccgctgc agccaccgcc cgcgggattg 
     2101 tgactgactt tgctttcctg agcccgcttg caagcagtgc agcttcccgt tcatccgccc 
     2161 gcgatgacaa gttgacggct cttttggcac aattggattc tttgacccgg gaacttaatg 
     2221 tcgtttctca gcagctgttg gatctgcgcc agcaggtttc tgccctgaag gcttcctccc 
     2281 ctcccaatgc ggtttaaaac ataaataaaa aaccagactc tgtttggatt tggatcaagc 
     2341 aagtgtcttg ctgtctttat ttaggggttt tgcgcgcgcg gtaggcccgg gaccagcggt 
     2401 ctcggtcgtt gagggtcctg tgtatttttt ccaggacgtg gtaaaggtga ctctggatgt 
     2461 tcagatacat gggcataagc ccgtctctgg ggtggaggta gcaccactgc agagcttcat 
     2521 gctgcggggt ggtgttgtag atgatccagt cgtagcagga gcgctgggcg tggtgcctaa 
     2581 aaatgtcttt cagtagcaag ctgattgcca ggggcaggcc cttggtgtaa gtgtttacaa 
     2641 agcggttaag ctgggatggg tgcatacgtg gggatatgag atgcatcttg gactgtattt 
     2701 ttaggttggc tatgttccca gccatatccc tccggggatt catgttgtgc agaaccacca 
     2761 gcacagtgta tccggtgcac ttgggaaatt tgtcatgtag cttagaagga aatgcgtgga 
     2821 agaacttgga gacgcccttg tgacctccaa gattttccat gcattcgtcc ataatgatgg 
     2881 caatgggccc acgggcggcg gcctgggcga agatatttct gggatcacta acgtcatagt 
     2941 tgtgttccag gatgagatcg tcataggcca tttttacaaa gcgcgggcgg agggtgccag 
     3001 actgcggtat aatggttcca tccggcccag gggcgtagtt accctcacag atttgcattt 
     3061 cccacgcttt gagttcagat ggggggatca tgtctacctg cggggcgatg aagaaaacgg 
     3121 tttccggggt aggggagatc agctgggaag aaagcaggtt cctgagcagc tgcgacttac 
     3181 cgcagccggt gggcccgtaa atcacaccta ttaccgggtg caactggtag ttaagagagc 
     3241 tgcagctgcc gtcatccctg agcagggggg ccacttcgtt aagcatgtcc ctgactcgca 
     3301 tgttttccct gaccaaatcc gccagaaggc gctcgccgcc cagcgatagc agttcttgca 
     3361 aggaagcaaa gtttttcaac ggtttgagac cgtccgccgt aggcatgctt ttgagcgttt 
     3421 gaccaagcag ttccaggcgg tcccacagct cggtcacctg ctctacggca tctcgatcca 
     3481 gcatatctcc tcgtttcgcg ggttggggcg gctttcgctg tacggcagta gtcggtgctc 
     3541 gtccagacgg gccagggtca tgtctttcca cgggcgcagg gtcctcgtca gcgtagtctg 
     3601 ggtcacggtg aaggggtgcg ctccgggctg cgcgctggcc agggtgcgct tgaggctggt 
     3661 cctgctggtg ctgaagcgct gccggtcttc gccctgcgcg tcggccaggt agcatttgac 
     3721 catggtgtca tagtccagcc cctccgcggc gtggcccttg gcgcgcagct tgcccttgga 
     3781 ggaggcgccg cacgaggggc agtgcagact tttgagggcg tagagcttgg gcgcgagaaa 
     3841 taccgattcc ggggagtagg catccgcgcc gcaggccccg cagacggtct cgcattccac 
     3901 gagccaggtg agctctggcc gttcggggtc aaaaaccagg tttcccccat gctttttgat 
     3961 gcgtttctta cctctggttt ccatgagccg gtgtccacgc tcggtgacga aaaggctgtc 
     4021 cgtgtccccg tatacagact tgagaggcct gtcctcgagc ggtgttccgc ggtcctcctc 
     4081 gtatagaaac tcggaccact ctgagacaaa ggctcgcgtc caggccagca cgaaggaggc 
     4141 taagtgggag gggtagcggt cgttgtccac tagggggtcc actcgctcca gggtgtgaag 
     4201 acacatgtcg ccctcttcgg catcaaggaa ggtgattggt ttgtaggtgt aggccacgtg 
     4261 accgggtgtt cctgaagggg ggctataaaa gggggtgggg gcgcgttcgt cctcactctc 
     4321 ttccgcatcg ctgtctgcga gggccagctg ttggggtgag tactccctct gaaaagcggg 
     4381 catgacttct gcgctaagat tgtcagtttc caaaaacgag gaggatttga tattcacctg 
     4441 gcccgcggtg atgcctttga gggtggccgc atccatctgg tcagaaaaga caatcttttt 
     4501 gttgtcaagc tagcttctat gggtttaaca atacctgtgt ggaagcctgg accgatgtaa 
     4561 gggttcgggg ctgtgccttt tactgctgct ggaagggggt ggtgtgtcgc cccaaaagca 
     4621 gggcttcaat taagaaatgc ctctttgaaa ggtgtacctt gggtatcctg tctgagggta 
     4681 actccagggt gcgccacaat gtggcctccg actgtggttg cttcatgcta gtgaaaagcg 
     4741 tggctgtgat taagcataac atggtatgtg gcaactgcga ggacagggcc tctcagatgc 
     4801 tgacctgctc ggacggcaac tgtcacctgc tgaagaccat tcacgtagcc agccactctc 
     4861 gcaaggcctg gccagtgttt gagcataaca tactgacccg ctgttccttg catttgggta 
     4921 acaggagggg ggtgttccta ccttaccaat gcaatttgag tcacactaag atattgcttg 
     4981 agcccgagag catgtccaag gtgaacctga acggggtgtt tgacatgacc atgaagatct 
     5041 gcgtctccgg tctcgccgct tagatcgctc tgtgtagtag ttgtagtata tccactctct 
     5101 caaagcatcc aggcgccccc tggcttcggg ttctatgtaa actccttcat gcgccgctgc 
     5161 cctgataaca tccaccaccg cagaataagc cacacccagc caacctacac attcgttctg 
     5221 cgagtcacac acgggaggag cgggaagagc tggaagaacc atgttttttt ttttattcca 
     5281 aaagattatc caaaacctca aaatgaagat cctctacgcc ggacgcatcg tggccggcat 
     5341 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga 
     5401 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 
     5461 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt 
     5521 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga 
     5581 gcgtcgatcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc 
     5641 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac 
     5701 aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga 
     5761 tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca 
     5821 ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg 
     5881 acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta 
     5941 tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc 
     6001 aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa 
     6061 cttcgatcac tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga 
     6121 acgggttggc atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc 
     6181 gcggtgcatg gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg 
     6241 attcaccact ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac 
     6301 caacccttgg cagaacatat ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat 
     6361 ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac 
     6421 ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg 
     6481 aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt 
     6541 cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgctcttcc gcttcctcgc 
     6601 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 
     6661 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 
     6721 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 
     6781 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 
     6841 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 
     6901 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 
     6961 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 
     7021 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 
     7081 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 
     7141 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 
     7201 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 
     7261 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 
     7321 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 
     7381 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 
     7441 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 
     7501 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 
     7561 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 
     7621 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 
     7681 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 
     7741 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 
     7801 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac 
     7861 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 
     7921 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 
     7981 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 
     8041 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 
     8101 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc 
     8161 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 
     8221 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 
     8281 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 
     8341 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 
     8401 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 
     8461 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 
     8521 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct 
     8581 ttcgtcttca agaattaatt ccatcatcaa taatatacct tattttggat tgaagccaat 
     8641 atgataatga gggggtggag tttgtgacgt ggcgcggggc gtgggaacgg ggcgggtgac 
     8701 gtagtagtgt ggcggaagtg tgatgttgca agtgtggcgg aacacatgta agcgacggat 
     8761 gtggcaaaag tgacgttttt ggtgtgcgcc ggtgtacaca ggaagtgaca attttcgcgc 
     8821 ggttttaggc ggatgttgta gtaaatttgg gcgtaaccga gtaagatttg gccattttcg 
     8881 cgggaaaact gaataagagg aagtgaaatc tgaataattt tgtgttactc atagcgcgta 
     8941 atatttgtct agggccgcgg ggactttgac cgtttacgtg gagactcgcc caggtgtttt 
     9001 tctcaggtgt tttccgcgtt ccgggtcaaa gttggcgttt tattattata gtcagtctag 
     9061 gcggccgcga tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat 
     9121 atagcataaa tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg 
     9181 tacatttata ttggctcatg tccaatatga ccgccatgtt gacattgatt attgactagt 
     9241 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt 
     9301 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg 
     9361 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaataa 
     9421 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 
     9481 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc 
     9541 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac 
     9601 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc 
     9661 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc 
     9721 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa 
     9781 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg 
     9841 tctatataag cagagctcgt ttagtgaacc gtcagatccg gtcgcgcg 
//
