ID pCAGGS-E-crmA standard; circular DNA; ; 5912 BP. DE LMBP 4888. CC Accession number: LMBP 4888 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 6-OCT-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_marker 16..54 FT /vntifkey="22" FT /label=E-tag FT /note="E-tag" FT CDS 64..1086 FT /vntifkey="4" FT /label=cowpox\virus\crmA FT terminator 1202..1293 FT /vntifkey="43" FT /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA FT misc_feature 1294..1650 FT /vntifkey="21" FT /label=3'\flanking\region\of\rabbit\ß-globin FT promoter complement(1728..1810) FT /vntifkey="30" FT /label=lac\promoter FT rep_origin 1952..2027 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT terminator 2058..2132 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(2058..2132) FT /vntifkey="43" FT /label=SV40\polyA\late FT rep_origin complement(2247..2648) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(3189..4049) FT /vntifkey="4" FT /label=ampicillin\resistance FT promoter 4185..4562 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT promoter 4568..5909 FT /vntifkey="29" FT /label=AG\promoter FT intron 4937..5861 FT /vntifkey="15" FT /label=intron\AG\promoter SQ Sequence 5912 BP; 1339 A; 1476 C; 1587 G; 1510 t; ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 60 gctgatatct tcagggaaat cgcatcttct atgaaaggag agaatgtatt catttctcca 120 ccgtcaatct cgtcagtatt gacaatactg tattatggag ctaatggatc cactgctgaa 180 cagctatcaa aatatgtaga aaaggaggcg gacaagaata aggatgatat ctcattcaag 240 tccatgaata aagtatatgg gcgatattct gcagtgttta aagattcctt tttgagaaaa 300 attggagata atttccaaac tgttgacttc actgattgtc gcactgtaga tgcgatcaac 360 aagtgtgttg atatcttcac tgaggggaaa attaatccac tattggatga accattgtct 420 ccagatacct gtctcctagc aattagtgcc gtatacttta aagcaaaatg gttgatgcca 480 tttgaaaagg aatttaccag tgattatccc ttttacgtat ctccaacgga aatggtagat 540 gtaagtatga tgtctatgta cggcgaggca tttaatcacg catctgtaaa agaatcattc 600 ggcaactttt caatcataga actgccatat gttggagata ctagtatggt ggtaattctt 660 ccagacaata ttgatggact agaatccata gaacaaaatc taacagatac aaattttaag 720 aaatggtgtg actctatgga tgctatgttt atcgatgtgc acattcccaa gtttaaggta 780 acaggctcgt ataatctggt ggatgcgcta gtaaagttgg gactgacaga ggtgttcggt 840 tcaactggag attatagcaa tatgtgtaat tcagatgtga gtgtcgacgc tatgatccac 900 aaaacgtata tagatgtcaa tgaagagtat acagaagcag ctgcagcaac ttgtgcgctg 960 gtggcagact gtgcatcaac agttacaaat gagttctgtg cagatcatcc gttcatctat 1020 gtgattaggc atgtcgatgg caaaattctt ttcgttggta gatattgctc tccaacaact 1080 aattaacccg ggacattaaa cgcgtgggta ccggatccgg caattcactc ctcaggtgca 1140 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg 1200 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac 1260 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct 1320 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt 1380 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag 1440 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt 1500 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct 1560 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt 1620 catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat 1680 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 1740 gagccggaag cataaagtgt aaagcctggg tgcctaatga gtgagctaac tcacattaat 1800 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat 1860 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg 1920 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 1980 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 2040 ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata 2100 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 2160 aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc 2220 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 2280 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 2340 cacagaatca gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 2400 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 2460 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 2520 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 2580 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 2640 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 2700 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 2760 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 2820 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 2880 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 2940 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 3000 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 3060 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 3120 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 3180 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 3240 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 3300 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 3360 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 3420 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 3480 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 3540 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 3600 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 3660 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 3720 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 3780 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 3840 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 3900 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 3960 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 4020 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 4080 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 4140 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ggtcgacatt gattattgac 4200 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 4260 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 4320 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 4380 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 4440 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 4500 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 4560 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc 4620 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg 4680 gggggggccc cccccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggaa 4740 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg 4800 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ttgcgcgctg 4860 ccttcccccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac 4920 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg 4980 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg 5040 ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg 5100 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg 5160 gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg 5220 cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc 5280 agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc 5340 tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt 5400 gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg 5460 ggagggctcg ggggaagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg 5520 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt 5580 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg 5640 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc 5700 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc 5760 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag 5820 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg 5880 ttattgtgct gtctcatcat tttggcaaag aa 5912 //