LOCUS       pAG25-AOX1-MFmds1E393A 7065 bp dsDNACircular
DEFINITION  LMBP 5073.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     CDS             complement(2305..3165)
                     /label=AMP
     promoter        40..382
                     /label=TEF1\promoter
     terminator      965..1162
                     /label=TEF1\terminator
     CDS             384..959
                     /label=NAT
     rep_origin      complement(1362..1764)
                     /label=pMB1\ori
     terminator      complement(3819..4078)
                     /label=AOX1\terminator
     misc_difference complement(5875..6117)
                     /label=ppMF
     misc_feature    complement(6129..6293)
                     /label=5'\UTR\of\AOX1
     promoter        complement(6294..7059)
                     /label=AOX1\promoter
     CDS             complement(4339..5838)
                     /label=mds1m
     CDS             complement(4339..6117)
                     /label=MFmds1E413A
ORIGIN
        1 gtttagcttg cctcgtcccc gccgggtcac ccggccagcg acatggaggc ccagaatacc 
       61 ctccttgaca gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg cccgtacatt 
      121 tagcccatac atccccatgt ataatcattt gcatccatac attttgatgg ccgcacggcg 
      181 cgaagcaaaa attacggctc ctcgctgcag acctgcgagc agggaaacgc tcccctcaca 
      241 gacgcgttga attgtcccca cgccgcgccc ctgtagagaa atataaaagg ttaggatttg 
      301 ccactgaggt tcttctttca tatacttcct tttaaaatct tgctaggata cagttctcac 
      361 atcacatccg aacataaaca accatgggta ccactcttga cgacacggct taccggtacc 
      421 gcaccagtgt cccgggggac gccgaggcca tcgaggcact ggatgggtcc ttcaccaccg 
      481 acaccgtctt ccgcgtcacc gccaccgggg acggcttcac cctgcgggag gtgccggtgg 
      541 acccgcccct gaccaaggtg ttccccgacg acgaatcgga cgacgaatcg gacgacgggg 
      601 aggacggcga cccggactcc cggacgttcg tcgcgtacgg ggacgacggc gacctggcgg 
      661 gcttcgtggt catctcgtac tcggcgtgga accgccggct gaccgtcgag gacatcgagg 
      721 tcgccccgga gcaccggggg cacggggtcg ggcgcgcgtt gatggggctc gcgacggagt 
      781 tcgccggcga gcggggcgcc gggcacctct ggctggaggt caccaacgtc aacgcaccgg 
      841 cgatccacgc gtaccggcgg atggggttca ccctctgcgg cctggacacc gccctgtacg 
      901 acggcaccgc ctcggacggc gagcggcagg cgctctacat gagcatgccc tgcccctaat 
      961 cagtactgac aataaaaaga ttcttgtttt caagaacttg tcatttgtat agttttttta 
     1021 tattgtagtt gttctatttt aatcaaatgt tagcgtgatt tatatttttt ttcgcctcga 
     1081 catcatctgc ccagatgcga agttaagtgc gcagaaagta atatcatgcg tcaatcgtat 
     1141 gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa cgccgccatc cagtgtcgaa 
     1201 aacgagctcg aattcatcga tgatatcaga tccactagtg gcctatgcgg ccgcggatct 
     1261 gccggtctcc ctatagtgag tcgtattaat ttcgataagc caggttaacc tgcattaatg 
     1321 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 
     1381 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 
     1441 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 
     1501 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 
     1561 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 
     1621 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 
     1681 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 
     1741 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 
     1801 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 
     1861 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 
     1921 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 
     1981 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 
     2041 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 
     2101 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 
     2161 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 
     2221 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 
     2281 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 
     2341 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 
     2401 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 
     2461 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 
     2521 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 
     2581 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 
     2641 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 
     2701 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 
     2761 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 
     2821 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 
     2881 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 
     2941 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 
     3001 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 
     3061 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 
     3121 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 
     3181 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 
     3241 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 
     3301 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 
     3361 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 
     3421 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 
     3481 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag 
     3541 agtgcaccat atggacatat tgtcgttaga acgcggctac aattaataca taaccttatg 
     3601 tatcatacac atacgattta ggtgacacta tagaacgcgg ccgccagatc ccgcaagagg 
     3661 cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg 
     3721 atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac 
     3781 tgtgataaac taccgcatta aagcttatcg ataagcttgc acaaacgaac ttctcactta 
     3841 atcttctgta ctctgaagag gagtgggaaa taccaagaaa aacatcaaac tcgaatgatt 
     3901 ttcccaaacc cctaccacaa gatattcatc agctgcgaga taggctgatc aggagcaagc 
     3961 tcgtacgaga agaaacaaaa tgacaaaaaa aatcctatac tatataggtt acaaataaaa 
     4021 aagtatcaaa aatgaagcct gcatctctca ggcaaatggc attctgacat cctcttgatt 
     4081 agaatctagc aagaccggtc ttctcgtaag tgcccaactt gaactgagga acagtcatgt 
     4141 ctaaggcgaa ttaattcgcg gccgcatcta gagggcccgg atctcttgcg cttcatatct 
     4201 cgaatgacat accatattcg gtggtcccag ccgctctatc cagacgcatg tgtcaaaata 
     4261 tctacggagc gattcgggac gagcagcaga aaatgaataa tacgagcaaa aaaacaggcg 
     4321 agagaactct cagcgccttt aagcaaggtg gccgccccgt cgtgatgatg aacggatgct 
     4381 aaaggggtgc gcctccgtgt taaagacaaa tttgttcccg ccggtggcct gcacctgcac 
     4441 atccgactcc tccgcaaaga tcaggtacgc atacttgagc gcctcggcaa accagaagct 
     4501 ctccatatcg tcagaggcac ccccgccgtt ggcctgcgtc acgtcgttga tggacgagta 
     4561 cgcgctgccg gcgcggcatg cgtcctcaat ggcactcaac gcttcccacg ccaggtcctg 
     4621 ccacttggag tcgcccgtga cgcggtatgc gtagtacaag ctctccagcg tcgccggccg 
     4681 caggatgtaa tacggtgccg tcacccagaa tcctgccgac gagtagaacc cggactggga 
     4741 cgagggcggc gagccgccgg cgcccgtcac gctgtccacc cacgcgaagc cttcggggcc 
     4801 gattccagaa gccgtctggg tgtacgtgcc aaagtacgag ctggcaagct tgattccaaa 
     4861 gtcaatgtac ttttgctcgt tcaggagaat gcctcccaag atgaagttgc caccgccaaa 
     4921 actggccaaa tgtcctgagt ttggcgacgt agactgtccg ttgtacgaag acaaaaaggt 
     4981 caagtccttg cgcgtcgacg ggtgagagcc gagatgccca atggtcgagt cggcaccaag 
     5041 gacccagcga tccttgtagt gtgcaaacgc aaccgggtcg tacaggtaca tcttgatcag 
     5101 gtactcgtag aagctgtcca tgaggccgga ccagctgccg ctgctatcct gaaaggtacc 
     5161 gttgctcgtg ctgacaaacg ttccaatcag gccaggccat gcctccgggc ttccctttgg 
     5221 attcaggaga tacgactcgc ccttctgcgc aagctgggca tactgcgggt ttcccgtcag 
     5281 gtcgctcaac cgtgtccact cgagcaccag gcttccaatt tcagcgacgt tgttgctaga 
     5341 tgcaccactt ctccggacag tagggttgaa gaagacggta gggtccggga caccgctggg 
     5401 agtggtgaac gcaaccttga ggccgttggc cagtgtttga gcctgcctca gaaggctgtt 
     5461 taccagggtc tggtttgtcg ccaaggagct gaaaggacct cgcaacaggt cataggcaga 
     5521 aagcaggcca ccgaggtacc gaatgttggt ctcgaacacg gaggatcctt ggttggcaac 
     5581 cgcagtcgtg gtgaagttga tctgcggtac atactgaagg atcgtgttca caatgtcggc 
     5641 atcccccatg aggatagccg tgtccaagcc atcgattgcc gacgagcccc agccgtttct 
     5701 ctcatcatca aagctgttgc tgaccgggtg gaggtcgtca tggggaaagg caaaatggtg 
     5761 gtaagcgttc cacgacgtct ggaatgcggc cttgactgct gccgccctcg tagggttggg 
     5821 agatccacgt tttgtggcga attctacgta agcttcagcc tctcttttct cgagagatac 
     5881 cccttcttct ttagcagcaa tgctggcaat agtagtattt ataaacaata acccgttatt 
     5941 tgtgctgttg gaaaatggca aaacagcaac atcgaaatcc ccttctaaat ctgagtaacc 
     6001 gatgacagct tcagccggaa tttgtgccgt ttcatcttct gttgtagtgt tgactggagc 
     6061 agctaatgcg gaggatgctg cgaataaaac tgcagtaaaa attgaaggaa atctcatcgt 
     6121 ttggatcctt cgaataatta gttgtttttt gatcttctca agttgtcgtt aaaagtcgtt 
     6181 aaaatcaaaa gcttgtcaat tggaaccagt cgcaattatg aaagtaagct aataatgatg 
     6241 ataaaaaaaa aggtttaaga cagggcagct tccttctgtt tatatattgc tgtcaagtag 
     6301 gggttagaac agttaaattt tgatcatgaa cgttaggcta tcagcagtat tcccaccaga 
     6361 atcttggaag catacaatgt ggagacaatg cataatcatc caaaaagcgg gtgtttcccc 
     6421 atttgcgttt cggcacaggt gcaccggggt tcagaagcga tagagagact gcgctaagca 
     6481 ttaatgagat tatttttgag cattcgtcaa tcaataccaa acaagacaaa cggtatggcg 
     6541 acttttggaa gtttcttttt gaccaactgg ccgttagcat ttcaacgaac caaacttagt 
     6601 tcatcttgga tgagatcacg cttttgtcat attaggttcc aagacagcgt ttaaactgtc 
     6661 agttttgggc catttgggga acatgaaact atttgacccc acactcagaa agccctcatc 
     6721 tggagtgatg ttcgggtgta atgcggagct tgttgcattc ggaaataaac aaacatgaac 
     6781 ctcgccaggg gggccaggat agacaggcta ataaagtcat ggtgttagta gcctaataga 
     6841 aggaattgga atgagcgagc tccaatcaag cccaataact gggctggttt ttcgatggca 
     6901 aaagtgggtg ttgaggagaa gaggagtgga ggtcctgcgt ttgcaacggt ctgctgctag 
     6961 tgtatcccct cctgttgcgt ttggcactta tgtgtgagaa tggacctgtg gatgtcggat 
     7021 ggcaaaaagg tttcattcaa cctttcgtct ttggatgtta gatct 
//
