LOCUS       pAG25-AOX1-MFhIL6 5978 bp dsDNACircular
DEFINITION  LMBP 5074.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     promoter        40..382
                     /label=TEF1\promoter
     CDS             384..959
                     /label=NAT
     terminator      965..1162
                     /label=TEF1\terminator
     rep_origin      complement(1362..1764)
                     /label=pMB1\ori
     CDS             complement(2305..3165)
                     /label=AMP
     promoter        complement(5207..5978)
                     /label=AOX1\promoter
     misc_feature    complement(5042..5206)
                     /label=5'\UTR\of\AOX1
     terminator      complement(3819..4078)
                     /label=AOX1\terminator
     misc_difference complement(4788..5030)
                     /label=ppMF
     CDS             complement(4218..4772)
                     /label=hIL6
ORIGIN
        1 gtttagcttg cctcgtcccc gccgggtcac ccggccagcg acatggaggc ccagaatacc 
       61 ctccttgaca gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg cccgtacatt 
      121 tagcccatac atccccatgt ataatcattt gcatccatac attttgatgg ccgcacggcg 
      181 cgaagcaaaa attacggctc ctcgctgcag acctgcgagc agggaaacgc tcccctcaca 
      241 gacgcgttga attgtcccca cgccgcgccc ctgtagagaa atataaaagg ttaggatttg 
      301 ccactgaggt tcttctttca tatacttcct tttaaaatct tgctaggata cagttctcac 
      361 atcacatccg aacataaaca accatgggta ccactcttga cgacacggct taccggtacc 
      421 gcaccagtgt cccgggggac gccgaggcca tcgaggcact ggatgggtcc ttcaccaccg 
      481 acaccgtctt ccgcgtcacc gccaccgggg acggcttcac cctgcgggag gtgccggtgg 
      541 acccgcccct gaccaaggtg ttccccgacg acgaatcgga cgacgaatcg gacgacgggg 
      601 aggacggcga cccggactcc cggacgttcg tcgcgtacgg ggacgacggc gacctggcgg 
      661 gcttcgtggt catctcgtac tcggcgtgga accgccggct gaccgtcgag gacatcgagg 
      721 tcgccccgga gcaccggggg cacggggtcg ggcgcgcgtt gatggggctc gcgacggagt 
      781 tcgccggcga gcggggcgcc gggcacctct ggctggaggt caccaacgtc aacgcaccgg 
      841 cgatccacgc gtaccggcgg atggggttca ccctctgcgg cctggacacc gccctgtacg 
      901 acggcaccgc ctcggacggc gagcggcagg cgctctacat gagcatgccc tgcccctaat 
      961 cagtactgac aataaaaaga ttcttgtttt caagaacttg tcatttgtat agttttttta 
     1021 tattgtagtt gttctatttt aatcaaatgt tagcgtgatt tatatttttt ttcgcctcga 
     1081 catcatctgc ccagatgcga agttaagtgc gcagaaagta atatcatgcg tcaatcgtat 
     1141 gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa cgccgccatc cagtgtcgaa 
     1201 aacgagctcg aattcatcga tgatatcaga tccactagtg gcctatgcgg ccgcggatct 
     1261 gccggtctcc ctatagtgag tcgtattaat ttcgataagc caggttaacc tgcattaatg 
     1321 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 
     1381 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 
     1441 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 
     1501 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 
     1561 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 
     1621 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 
     1681 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 
     1741 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 
     1801 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 
     1861 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 
     1921 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 
     1981 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 
     2041 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 
     2101 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 
     2161 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 
     2221 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 
     2281 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 
     2341 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 
     2401 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 
     2461 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 
     2521 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 
     2581 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 
     2641 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 
     2701 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 
     2761 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 
     2821 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 
     2881 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 
     2941 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 
     3001 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 
     3061 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 
     3121 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 
     3181 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 
     3241 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 
     3301 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 
     3361 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 
     3421 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 
     3481 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag 
     3541 agtgcaccat atggacatat tgtcgttaga acgcggctac aattaataca taaccttatg 
     3601 tatcatacac atacgattta ggtgacacta tagaacgcgg ccgccagatc ccgcaagagg 
     3661 cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg 
     3721 atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac 
     3781 tgtgataaac taccgcatta aagcttatcg ataagcttgc acaaacgaac ttctcactta 
     3841 atcttctgta ctctgaagag gagtgggaaa taccaagaaa aacatcaaac tcgaatgatt 
     3901 ttcccaaacc cctaccacaa gatattcatc agctgcgaga taggctgatc aggagcaagc 
     3961 tcgtacgaga agaaacaaaa tgacaaaaaa aatcctatac tatataggtt acaaataaaa 
     4021 aagtatcaaa aatgaagcct gcatctctca ggcaaatggc attctgacat cctcttgatt 
     4081 agaatctagc aagaccggtc ttctcgtaag tgcccaactt gaactgagga acagtcatgt 
     4141 ctaaggcgaa ttaattcgcg gccgccctag ggaacacgcg tggcgcgcat taacaacaac 
     4201 aatctgaggt gcccatgcta catttgccga agagccctca ggctggactg caggaactcc 
     4261 ttaaagctgc gcagaatgag atgagttgtc atgtcctgca gccactggtt ctgtgcctgc 
     4321 agcttcgtca gcaggctggc atttgtggtt gggtcagggg tggttattgc atctagattc 
     4381 tttgcctttt tctgcaggaa ctggatcagg acttttgtac tcatctgcac agctctggct 
     4441 tgttcctcac tactctcaaa tctgttctgg aggtactcta ggtatacctc aaactccaaa 
     4501 agaccagtga tgattttcac caggcaagtc tcctcattga atccagattg gaagcatcca 
     4561 tctttttcag ccatctttgg aaggttcagg ttgttttctg ccagtgcctc tttgctgctt 
     4621 tcacacatgt tactcttgtt acatgtctcc tttctcaggg ctgagatgcc gtcgaggatg 
     4681 taccgaattt gtttgtcaat tcgttctgaa gaggtgagtg gctgtctgtg tggggcggct 
     4741 acatctttgg aatcttctcc tgggggtaca ggcgcgcgcg ccccgagaga taccccttct 
     4801 tctttagcag caatgctggc aatagtagta tttataaaca ataacccgtt atttgtgctg 
     4861 ttggaaaatg gcaaaacagc aacatcgaaa tccccttcta aatctgagta accgatgaca 
     4921 gcttcagccg gaatttgtgc cgtttcatct tctgttgtag tgttgactgg agcagctaat 
     4981 gcggaggatg ctgcgaataa aactgcagta aaaattgaag gaaatctcat cgtttggatc 
     5041 cttcgaataa ttagttgttt tttgatcttc tcaagttgtc gttaaaagtc gttaaaatca 
     5101 aaagcttgtc aattggaacc agtcgcaatt atgaaagtaa gctaataatg atgataaaaa 
     5161 aaaaggttta agacagggca gcttccttct gtttatatat tgctgtcaag taggggttag 
     5221 aacagttaaa ttttgatcat gaacgttagg ctatcagcag tattcccacc agaatcttgg 
     5281 aagcatacaa tgtggagaca atgcataatc atccaaaaag cgggtgtttc cccatttgcg 
     5341 tttcggcaca ggtgcaccgg ggttcagaag cgatagagag actgcgctaa gcattaatga 
     5401 gattattttt gagcattcgt caatcaatac caaacaagac aaacggtatg gcgacttttg 
     5461 gaagtttctt tttgaccaac tggccgttag catttcaacg aaccaaactt agttcatctt 
     5521 ggatgagatc acgcttttgt catattaggt tccaagacag cgtttaaact gtcagttttg 
     5581 ggccatttgg ggaacatgaa actatttgac cccacactca gaaagccctc atctggagtg 
     5641 atgttcgggt gtaatgcgga gcttgttgca ttcggaaata aacaaacatg aacctcgcca 
     5701 ggggggccag gatagacagg ctaataaagt catggtgtta gtagcctaat agaaggaatt 
     5761 ggaatgagcg agctccaatc aagcccaata actgggctgg tttttcgatg gcaaaagtgg 
     5821 gtgttgagga gaagaggagt ggaggtcctg cgtttgcaac ggtctgctgc tagtgtatcc 
     5881 cctcctgttg cgtttggcac ttatgtgtga gaatggacct gtggatgtcg gatggcaaaa 
     5941 aggtttcatt caacctttcg tctttggatg ttagatct 
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