LOCUS       pCAGGS-E-hABIN1-N 5989 bp dsDNACircular
DEFINITION  LMBP 5128.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     misc_difference 11..49
                     /label=E-tag
     CDS             59..1186
                     /label=hABIN1-N
     rep_origin      2024..2099
                     /label=SV40\ori
     promoter        complement(1800..1882)
                     /label=lac\promoter
     terminator      1274..1365
                     /label=ß-globin\polyA
     rep_origin      complement(2319..2720)
                     /label=pMB1\ori
     terminator      2130..2204
                     /label=SV40\polyA\early
     terminator      complement(2130..2204)
                     /label=SV40\polyA\late
     misc_feature    1366..1722
                     /label=3'\FR\of\ß-globin
     promoter        4640..5981
                     /label=AG\promoter
     promoter        4257..4634
                     /label=hCMV\enhancer
     intron          5009..5933
                     /label=intron\AG\promoter
     CDS             complement(3261..4121)
                     /label=AMP
ORIGIN
        1 ttccaccatg ggtgcgccgg tgccgtatcc ggacccgctg gaaccgcgtg cggccgccat 
       61 ggaagggaga ggaccgtacc ggatttacga ccctgggggc agcgtgccct caggagaggc 
      121 atccgcagct tttgagcgcc tagtgaagga gaattcccgg ctgaaggaaa aaatgcaagg 
      181 gataaagatg ttaggggagc ttttggaaga gtcccagatg gaagcgacca ggctccggca 
      241 gaaggcagag gagctagtga aggacaacga gctgctccca ccaccttctc cctccttggg 
      301 ctccttcgac cccctggctg agctcacagg aaaggactca aatgtcacag catctcccac 
      361 agcccctgca tgccccagtg acaagccagc accagtccag aagcctccat ccagtggcac 
      421 ctcctctgaa tttgaagtgg tcactcctga ggagcagaat tcaccagaga gcagcagcca 
      481 tgccaatgcg atggcgctgg gccccctgcc ccgtgaggac ggcaacctga tgctgcacct 
      541 gcagcgcctg gagaccacgc tgagtgtgtg tgccgaggag ccggaccacg gccagctctt 
      601 cacccacctg ggccgcatgg ccctggagtt caaccgactg gcatccaagg tgcacaagaa 
      661 tgagcagcgc acctccattc tgcagaccct gtgtgagcag cttcggaagg agaacgaggc 
      721 tctgaaggcc aagttggata agggcctgga acagcgggat caggctgccg agaggctgcg 
      781 ggaggaaaat ttggagctca agaagttgtt gatgagcaat ggcaacaaag agggtgcgtc 
      841 tgggcggcca ggctcaccga agatggaagg gacaggcaag aaggcagcgg ctggacagca 
      901 gcaggctagt gtgacggcag gtaaggtccc agaggtggtg gccttgggcg cagccgagaa 
      961 gaaggtgaag atgctggagc agcagcgcag tgagctgctg gaagtgaaca agcagtggga 
     1021 ccagcatttc cggtccatga agcagcagta tgagcagaag atcactgagc tgcgtcagaa 
     1081 gctggctgat ttgcagaagc aggtgactga cctggaggcc gagcgggagc agaagcagcg 
     1141 tgactttgac cgcaagctcc tcctggccaa gtccaagatt gaatgactcg aggaattcac 
     1201 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca 
     1261 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct 
     1321 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa 
     1381 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa 
     1441 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg 
     1501 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca 
     1561 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc 
     1621 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg 
     1681 actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa 
     1741 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 
     1801 cacacaacat acgagccgga agcataaagt gtaaagcctg ggtgcctaat gagtgagcta 
     1861 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 
     1921 gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 
     1981 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 
     2041 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 
     2101 tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca 
     2161 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 
     2221 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat 
     2281 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 
     2341 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 
     2401 aatacggtta tccacagaat cagggataac gcaggaaaga acatgtgagc aaaaggccag 
     2461 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 
     2521 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 
     2581 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 
     2641 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 
     2701 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 
     2761 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 
     2821 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 
     2881 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 
     2941 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 
     3001 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 
     3061 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 
     3121 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 
     3181 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 
     3241 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 
     3301 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 
     3361 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 
     3421 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 
     3481 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 
     3541 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 
     3601 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 
     3661 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 
     3721 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 
     3781 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 
     3841 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 
     3901 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 
     3961 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 
     4021 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 
     4081 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 
     4141 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 
     4201 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtcgaca 
     4261 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata 
     4321 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga 
     4381 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 
     4441 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt 
     4501 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca 
     4561 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt 
     4621 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc 
     4681 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg 
     4741 ggcggggggg gggggggggc cccccccagg cggggcgggg cggggcgagg ggcggggcgg 
     4801 ggcgaggcgg aaaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt 
     4861 tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt 
     4921 cgttgcgcgc tgccttcccc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg 
     4981 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg 
     5041 ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct 
     5101 tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg 
     5161 tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg 
     5221 gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg 
     5281 tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt 
     5341 gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc 
     5401 tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc 
     5461 ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc 
     5521 gcctcgggcc ggggagggct cgggggaagg ggcgcggcgg cccccggagc gccggcggct 
     5581 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg 
     5641 gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct 
     5701 ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc 
     5761 ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg 
     5821 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg 
     5881 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg 
     5941 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattgaa 
//
