LOCUS       pCAGGS-Flag-hABIN1-P299A 6755 bp dsDNACircular
DEFINITION  LMBP 5132.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     misc_difference 13..36
                     /label=FLAG-tag
     rep_origin      2791..2866
                     /label=SV40\ori
     promoter        complement(2567..2649)
                     /label=lac\promoter
     terminator      2041..2132
                     /label=ß-globin\polyA
     rep_origin      complement(3086..3487)
                     /label=pMB1\ori
     terminator      2897..2971
                     /label=SV40\polyA\early
     terminator      complement(2897..2971)
                     /label=SV40\polyA\late
     misc_feature    2133..2489
                     /label=3'\FR\of\ß-globin
     promoter        5028..5405
                     /label=hCMV\enhancer
     promoter        5411..6752
                     /label=AG\promoter
     intron          5780..6704
                     /label=intron\AG\promoter
     CDS             43..1953
                     /label=hABIN1m
     CDS             complement(4028..4888)
                     /label=AMP
ORIGIN
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggaagg gagaggaccg 
       61 taccggattt acgaccctgg gggcagcgtg ccctcaggag aggcatccgc agcttttgag 
      121 cgcctagtga aggagaattc ccggctgaag gaaaaaatgc aagggataaa gatgttaggg 
      181 gagcttttgg aagagtccca gatggaagcg accaggctcc ggcagaaggc agaggagcta 
      241 gtgaaggaca acgagctgct cccaccacct tctccctcct tgggctcctt cgaccccctg 
      301 gctgagctca caggaaagga ctcaaatgtc acagcatctc ccacagcccc tgcatgcccc 
      361 agtgacaagc cagcaccagt ccagaagcct ccatccagtg gcacctcctc tgaatttgaa 
      421 gtggtcactc ctgaggagca gaattcacca gagagcagca gccatgccaa tgcgatggcg 
      481 ctgggccccc tgccccgtga ggacggcaac ctgatgctgc acctgcagcg cctggagacc 
      541 acgctgagtg tgtgtgccga ggagccggac cacggccagc tcttcaccca cctgggccgc 
      601 atggccctgg agttcaaccg actggcatcc aaggtgcaca agaatgagca gcgcacctcc 
      661 attctgcaga ccctgtgtga gcagcttcgg aaggagaacg aggctctgaa ggccaagttg 
      721 gataagggcc tggaacagcg ggatcaggct gccgagaggc tgcgggagga aaatttggag 
      781 ctcaagaagt tgttgatgag caatggcaac aaagagggtg cgtctgggcg gccaggctca 
      841 ccgaagatgg aagggacagg caagaaggca gtggctggac agcagcaggc tagtgtgacg 
      901 gcaggtaagg tcccagaggt ggtggccttg ggcgcagccg agaagaaggt gaagatgctg 
      961 gagcagcagc gcagtgagct gctggaagtg aacaagcagt gggaccagca tttccggtcc 
     1021 atgaagcagc agtatgagca gaagatcact gagctgcgtc agaagctggc tgatttgcag 
     1081 aagcaggtga ctgacctgga ggccgagcgg gagcagaagc agcgtgactt tgaccgcaag 
     1141 ctcctcctgg ccaagtccaa gattgaaatg gaggagaccg acaaggagca gctgacagca 
     1201 gaggccaagg agctgcgcca aaaggtcaag tacctgcagg atcagctgag cccactcacc 
     1261 cgacagcgtg agtaccagga aaaggagatc cagcggctca acaaggccct ggaggaagca 
     1321 ctgagcatcc aaaccccgcc atcatctcca ccaacagcat ttgggagccc agaaggagca 
     1381 ggggccctcc taaggaaaca ggagctggtc acgcagaatg agttgctgaa acagcaggtg 
     1441 aagatcttcg aggaggactt ccagagggag cgcagtgatc gtgagcgcat gaatgaggag 
     1501 aaggaagagc tgaagaagca agtggagaag ctgcaggccc aggtcaccct gtcaaatgcc 
     1561 cagctaaaag cattcaaaga tgaggagaag gcaagagaag ccctcagaca gcagaagagg 
     1621 aaagcaaagg cctcaggaga gcgttaccat gtggagcccc acccagaaca tctctgcggg 
     1681 gcctacccct acgcctaccc gcccatgcca gccatggtgc cacaccatgg cttcgaggac 
     1741 tggtcccaga tccgctaccc ccctcccccc atggccatgg agcacccgcc cccactcccc 
     1801 aactcgcgcc tcttccatct gccggaatac acctggcgtc taccctgtgg aggggttcga 
     1861 aatccaaatc agagctccca agtgatggac cctcccacag ccaggcctac agaaccagag 
     1921 tctccaaaaa atgaccgtga ggggcctcag tgactcgagg aattcactcc tcaggtgcag 
     1981 gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga 
     2041 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact 
     2101 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc 
     2161 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt 
     2221 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga 
     2281 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg 
     2341 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta 
     2401 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc 
     2461 atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgtaatc 
     2521 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 
     2581 agccggaagc ataaagtgta aagcctgggt gcctaatgag tgagctaact cacattaatt 
     2641 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc 
     2701 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 
     2761 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 
     2821 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 
     2881 gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat aaagcaatag 
     2941 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 
     3001 actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg 
     3061 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 
     3121 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 
     3181 acagaatcag ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 
     3241 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 
     3301 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 
     3361 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 
     3421 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 
     3481 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 
     3541 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 
     3601 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 
     3661 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 
     3721 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 
     3781 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 
     3841 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 
     3901 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 
     3961 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 
     4021 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 
     4081 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 
     4141 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 
     4201 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 
     4261 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 
     4321 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 
     4381 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 
     4441 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 
     4501 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 
     4561 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 
     4621 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 
     4681 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 
     4741 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 
     4801 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 
     4861 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 
     4921 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 
     4981 taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgatcgac attgattatt 
     5041 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt 
     5101 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc 
     5161 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg 
     5221 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat 
     5281 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca 
     5341 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat 
     5401 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc 
     5461 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg 
     5521 gggggggggg ccccccccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg 
     5581 gaaaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag 
     5641 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgttgcgcg 
     5701 ctgccttccc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact 
     5761 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta 
     5821 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct 
     5881 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt 
     5941 ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc 
     6001 ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg 
     6061 gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg 
     6121 agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt 
     6181 tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc 
     6241 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc 
     6301 cggggagggc tcgggggaag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg 
     6361 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt 
     6421 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc 
     6481 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt 
     6541 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct 
     6601 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta 
     6661 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc 
     6721 tggttattgt gctgtctcat cattttggca aagaa 
//
