LOCUS       pCAGGS-E-hMKP1-P        5894 bp    DNA     circular     18-SEP-2017
DEFINITION  LMBP 5140.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5140
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 15-SEP-2017
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|-11945596|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCAGGS-E-hMKP1-P142H-P360R|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Virus
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-E-hMKP1-P142H-P360R" 0 5894 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "HincII" "HindIII" "NcoI" "BglII" "XbaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5894 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "HincII" "gtyrac" 4 0 3 2984 0 4910 0 5197 0 0 
COMMENT            "") #1=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 467 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 5 744 0 3360 0 4723 0 4954 
COMMENT            0 5752 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "BglII" "agatct" 2 0 1 1 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 4614 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "3' UTR of rabbit \337-globin, incl. polyA" 43 0 
COMMENT            0 1 92 0 (CStringList) (CStringList) 1 1 1 1 "1..92") 
COMMENT        #6=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 0 
COMMENT            93 449 0 (CStringList) (CStringList) 1 1 1 1 "93..449") 
COMMENT        #7=(CFSignal (CObList) "lac promoter" 30 0 1 527 609 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(527..609)") 
COMMENT        #8=(CFSignal (CObList) "SV40 ori" 33 0 0 751 826 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "751..826") 
COMMENT        #9=(CFSignal (CObList) "SV40 polyA early" 43 0 0 857 931 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "857..931") 
COMMENT        #10=(CFSignal (CObList) "SV40 polyA late" 43 0 1 857 931 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(857..931)") 
COMMENT        #11=(CFSignal (CObList) "pMB1 ori" 33 0 1 1046 1447 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1046..1447)") 
COMMENT        #12=(CFSignal (CObList) "ampicillin resistance" 4 0 1 1988 2848 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(1988..2848)") 
COMMENT        #13=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 2984 3361 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2984..3361") 
COMMENT        #14=(CFSignal (CObList) "AG promoter" 29 0 0 3367 4708 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3367..4708") 
COMMENT        #15=(CFSignal (CObList) "intron AG promoter" 15 0 0 3736 4660 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3736..4660") 
COMMENT        #16=(CFSignal (CObList) "E-tag" 22 0 0 4727 4765 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4727..4765") 
COMMENT        #17=(CFSignal (CObList) "hDUSP1" 4 0 0 4781 5881 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4781..5881") 
COMMENT        #18=(CFSignal (CObList) "P142H" 62 0 0 5201 5203 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #19=(CFSignal (CObList) "P360R" 62 0 0 5855 5857 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #20=(CFSignal (CObList) "LMBP 5140" 2 0 0 0 0 0 (CStringList) 
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COMMENT                          "DNA Plasmid Virus 'pCAGGS-E-hMKP1-P142H-P360R'" 1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                          "Last Modified: 09/18/17 12:31" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5894 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "Modification Date in the Original DB: 15-SEP-2017" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "TEL: 0032-9-33-13-843  " 1) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
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COMMENT                          "WWW: <a href=\"http://www.genecorner.ugent.be\">www.plasmids-lmbp.ugent.be/</a>" 
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COMMENT                          "Promoter Eukaryotic (2 total)") 
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FEATURES             Location/Qualifiers
     terminator      1..92
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_feature    93..449
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     promoter        complement(527..609)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      751..826
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      857..931
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(857..931)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(1046..1447)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(1988..2848)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        2984..3361
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     promoter        3367..4708
                     /vntifkey="29"
                     /label=AG\promoter
     intron          3736..4660
                     /vntifkey="15"
                     /label=intron\AG\promoter
     misc_marker     4727..4765
                     /vntifkey="22"
                     /label=E-tag
     CDS             4781..5881
                     /vntifkey="4"
                     /label=hDUSP1
     mutation        5201..5203
                     /vntifkey="62"
                     /label=P142H
                     /note="WT: CCC"
     mutation        5855..5857
                     /vntifkey="62"
                     /label=P360R
                     /note="WT: CCC"
BASE COUNT     1196 a      1641 c      1655 g      1402 t 
ORIGIN
        1 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact 
       61 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc 
      121 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt 
      181 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga 
      241 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg 
      301 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta 
      361 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc 
      421 atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgtaatc 
      481 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 
      541 agccggaagc ataaagtgta aagcctgggt gcctaatgag tgagctaact cacattaatt 
      601 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc 
      661 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 
      721 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 
      781 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 
      841 gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat aaagcaatag 
      901 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 
      961 actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg 
     1021 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 
     1081 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 
     1141 acagaatcag ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 
     1201 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 
     1261 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 
     1321 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 
     1381 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 
     1441 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 
     1501 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 
     1561 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 
     1621 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 
     1681 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 
     1741 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 
     1801 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 
     1861 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 
     1921 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 
     1981 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 
     2041 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 
     2101 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 
     2161 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 
     2221 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 
     2281 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 
     2341 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 
     2401 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 
     2461 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 
     2521 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 
     2581 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 
     2641 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 
     2701 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 
     2761 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 
     2821 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 
     2881 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 
     2941 taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgacattg attattgact 
     3001 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 
     3061 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 
     3121 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 
     3181 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 
     3241 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 
     3301 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 
     3361 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc 
     3421 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg 
     3481 ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaaa 
     3541 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg 
     3601 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgt tgcgcgctgc 
     3661 cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc 
     3721 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc 
     3781 ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg 
     3841 gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg 
     3901 agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg 
     3961 ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc 
     4021 ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca 
     4081 gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct 
     4141 gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg 
     4201 ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg 
     4261 gagggctcgg gggaaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc 
     4321 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc 
     4381 ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg 
     4441 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc 
     4501 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct 
     4561 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc 
     4621 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt 
     4681 tattgtgctg tctcatcatt ttggcaaaga attgaattcc accatgggtg cgccggtgcc 
     4741 gtatccggac ccgctggaac cgcgtgcggc cgcgaattcg gtcatggaag tgggcaccct 
     4801 ggacgctgga ggcctgcggg cgctgctggg ggagcgagcg gcgcaatgcc tgctgctgga 
     4861 ctgccgctcc ttcttcgctt tcaacgccgg ccacatcgcc ggctctgtca acgtgcgctt 
     4921 cagcaccatc gtgcggcgcc gggccaaggg cgccatgggc ctggagcaca tcgtgcccaa 
     4981 cgccgagctc cgcggccgcc tgctggccgg cgcctaccac gccgtggtgt tgctggacga 
     5041 gcgcagcgcc gccctggacg gcgccaagcg cgacggcacc ctggccctgg cggccggcgc 
     5101 gctctgccgc gaggcgcgcg ccgcgcaagt cttcttcctc aaaggaggat acgaagcgtt 
     5161 ttcggcttcc tgcccggagc tgtgcagcaa acagtcgacc cacatggggc tcagccttcc 
     5221 cctgagtact agcgtccctg acagcgcgga atctgggtgc agttcctgca gtaccccact 
     5281 ctacgatcag ggtggcccgg tggaaatcct gccctttctg tacctgggca gtgcgtatca 
     5341 cgcttcccgc aaggacatgc tggatgcctt gggcataact gccttgatca acgtctcagc 
     5401 caattgtccc aaccattttg agggtcacta ccagtacaag agcatccctg tggaggacaa 
     5461 ccacaaggca gacatcagct cctggttcaa cgaggccatt gacttcatag actccatcaa 
     5521 gaatgctgga ggaagggtgt ttgtccactg ccaggcgggc atttcccggt cagccaccat 
     5581 ctgccttgct taccttatga ggactaatcg agtcaagctg gacgaggcct ttgagtttgt 
     5641 gaagcagagg cgaagcatca tctctcccaa cttcagcttc atgggccagc tgctgcagtt 
     5701 tgagtcccaa gtgctggctc cgcactgttc ggcagaggct gggagccccg ccatggctgt 
     5761 gctcgaccga ggcacctcca ccaccaccgt gttcaacttc cccgtctcca tccctgtcca 
     5821 ctccacgaac agtgcgctga gctaccttca gagccgcatt acgacctctc ccagctgctg 
     5881 agaattcatc gata 
//
