LOCUS       pBBR-exoS-GAPmin        6300 bp    DNA     circular     18-JUL-2013
DEFINITION  LMBP 5159.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5159
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 18-JUL-2013
            
COMMENT     VNTDATE|492531129|
COMMENT     VNTNAME|pBBR-exoS-GAPmin|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBBR-exoS-GAPmin" 0 6300 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "BamHI" "XbaI" "BglI" "MlsI" "NcoI" "PstI" "PvuI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 5970 5969 1 6300 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 4845 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 3240 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 6 67 0 319 0 944 0 
COMMENT            2296 0 3035 0 4167 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "MlsI" "tggcca" 4 0 5 1797 0 3879 0 4471 0 4714 
COMMENT            0 5347 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 5309 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 3784 0 4009 0 4861 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 3063 0 0 "")) 
COMMENT       (CObList 
COMMENT        #7=(CFSignal (CObList) "pBBR1 ori" 33 0 0 1793 2455 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1793..2455") 
COMMENT        #8=(CFSignal (CObList) "T7 promoter" 30 0 0 3188 3204 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3188..3204") 
COMMENT        #9=(CFSignal (CObList) "chloramphenicol resistance" 4 0 1 5138 5827 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(5138..5827)") 
COMMENT        #10=(CFSignal (CObList) "lac promoter" 30 0 1 5010 5093 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5010..5093)") 
COMMENT        #11=(CFSignal (CObList) "T3 promoter" 30 0 1 4930 4946 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4930..4946)") 
COMMENT        #12=(CFSignal (CObList) "mob" 4 0 1 6080 799 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(6080..799)") 
COMMENT        #13=(CFSignal (CObList) "exoS mutant" 4 0 0 3464 4822 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3464..4822") 
COMMENT        #14=(CFSignal (CObList) "His-tag" 22 0 0 4823 4840 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4823..4840") 
COMMENT        #15=(CFSignal (CObList) "R146K" 62 0 0 3899 3901 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3899..3901") 
COMMENT        #16=(CFSignal (CObList) "exoS promoter" 30 0 0 3245 3463 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3245..3463")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList 
COMMENT        #17=(CFSignal (CObList) "LMBP 5159" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #18=(CFSignal (CObList) "Last modified: 18-JUL-2013" 2 0 0 0 0 0 
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COMMENT                    2) 5 "" 0 4) 
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COMMENT                          "DNA Plasmid 'pBBR-exoS-GAPmin'" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 5159." 
COMMENT                          1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 09/04/08 02:12" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 07/18/13 05:24" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6300 bp" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "Original Source Database: GenBank" 1) 
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COMMENT                          "Modification Date in the Original DB: 18-JUL-2013" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
COMMENT                          1))) "Original Author") 
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COMMENT                                 "" 1))) "LMBP 5159") 
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COMMENT                   "Annotations") 
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COMMENT                                        "Start: 3464  End: 4822" 1) 
COMMENT                                   #46=(CLinePar 
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COMMENT                                        "   3464..4822" 1))) "exoS mutant") 
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COMMENT                                        "Start: 5138  End: 5827 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #50=(CLinePar 
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COMMENT                                        "10 C-terminal codons are missing, alternative stop codon is present 30 bp beyond the normal length" 
COMMENT                                        1) 
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COMMENT                                        "   complement(5138..5827)" 1))) 
COMMENT                                 "chloramphenicol resistance") 
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COMMENT                          "CDS (3 total)") 
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COMMENT                          "Misc. Marker (1 total)") 
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COMMENT                                   #65=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3188..3204" 1))) "T7 promoter") 
COMMENT                            #67=(CFolderPar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #70=(CLinePar 
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COMMENT                                        "   3245..3463" 1))) "exoS promoter") 
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COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4930  End: 4946 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #73=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(4930..4946)" 1))) 
COMMENT                                 "T3 promoter") 
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COMMENT                                        "Start: 5010  End: 5093 (Complementary)" 
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COMMENT                                   #77=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(5010..5093)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (4 total)") 
COMMENT                     #79=(CFolderPar 
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COMMENT                                        "Start: 1793  End: 2455" 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "vegetative replication origin; incl. rep gene" 
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COMMENT                                   #83=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #84=(CLinePar 
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COMMENT                                        "   1793..2455" 1))) "pBBR1 ori"))) 
COMMENT                          "Replication Origin (1 total)") 
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COMMENT                                        "Start: 3899  End: 3901" 1) 
COMMENT                                   #88=(CLinePar 
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COMMENT                                        "WT: CGT" 1) 
COMMENT                                   #89=(CLinePar 
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COMMENT                                        "   3899..3901" 1))) "R146K"))) 
COMMENT                          "Mutation (1 total)"))) "Feature Map") 
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COMMENT                                        " N1: 4845 " 1))))) "BamHI: 1 site") 1 5 
COMMENT                          "GGATCC" "CCTAGG") 
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COMMENT                          "TCTAGA" "AGATCT") 
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COMMENT                          "CCATGG" "GGTACC") 
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COMMENT                                           " N6: 4167 " 1))))) "BglI: 6 sites") 
COMMENT                           7 4 "GCCNNNNNGGC" "CGGNNNNNCCG"))) 
COMMENT                   "Restriction/Methylation Map")))) 
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FEATURES             Location/Qualifiers
     rep_origin      1793..2455
                     /vntifkey="33"
                     /label=pBBR1\ori
                     /note="vegetative replication origin; incl. rep gene"
     promoter        3188..3204
                     /vntifkey="30"
                     /label=T7\promoter
     CDS             complement(5138..5827)
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
                     /note="10 C-terminal codons are missing, alternative stop codon is present 30 bp beyond the normal length"
     promoter        complement(5010..5093)
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        complement(4930..4946)
                     /vntifkey="30"
                     /label=T3\promoter
     CDS             complement(6080..799)
                     /vntifkey="4"
                     /label=mob
     CDS             3464..4822
                     /vntifkey="4"
                     /label=exoS\mutant
     misc_marker     4823..4840
                     /vntifkey="22"
                     /label=His-tag
     mutation        3899..3901
                     /vntifkey="62"
                     /label=R146K
                     /note="WT: CGT"
     promoter        3245..3463
                     /vntifkey="30"
                     /label=exoS\promoter
BASE COUNT     1290 a      1835 c      1872 g      1303 t 
ORIGIN
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg 
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt 
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg 
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg 
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca 
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt 
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg 
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt 
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg 
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct 
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg 
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc 
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca 
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg 
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg 
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc 
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc 
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga 
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc 
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt 
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg 
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac 
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc 
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg 
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg 
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag 
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta 
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg 
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc 
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac 
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga 
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct 
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac 
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt 
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt 
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag 
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct 
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga 
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac 
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct 
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt 
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg 
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga 
     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga 
     2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg 
     2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa 
     2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa 
     2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag 
     2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc 
     2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 
     3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg 
     3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 
     3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga 
     3181 gcgcgcgtaa tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc 
     3241 tagatggtgg atggcggcgc ggtagagtgg attcatggcg tgttccgagt cactggaggc 
     3301 agccattaga gcagtgccag cccggagaga ctgttaatcg tggttctctt tttttaggtt 
     3361 ttgccgctgc cgattccagt gaaaaaacgg cggccaatcc tgataggcga tggggtttcc 
     3421 cgttcctaga ctggcggaga aacatcagga gaaggcaacc atcatgcata ttcaatcgct 
     3481 tcagcagagt ccgtctttcg ccgtcgaatt gcaccaggcc gccagtgggc gtttgggaca 
     3541 gattgaggcc cgccaggtcg ccacccccag tgaagcgcag cagttggccc agcgccagga 
     3601 cgcgccgaag ggtgaggggc tgctcgctcg cttgggtgcg gcactcgtgc gtcccttcgt 
     3661 ggcgatcatg gactggctgg gcaagctgtt gggctcccac gcccgcaccg gcccgcagcc 
     3721 cagtcaggac gcgcagcctg cggtcatgtc ctcggccgtc gtgttcaagc agatggtgct 
     3781 gcagcaggca ttgcccatga ccttgaaggg actcgacaag gcgagcgagc tggcgaccct 
     3841 gacaccggaa ggactggccc gggagcactc ccgcctggcc agcggagatg gggcgctgaa 
     3901 atcgctgagc accgccttgg ccggcattcg tgccggcagc caggtcgagg agtcccgtat 
     3961 ccaggctggc cgcctgctcg aacggagcat cggcgggatc gcgctgcagc agtggggcac 
     4021 caccggcggt gccgcgagtc aactggtgct cgacgcaagc ccggaactgc ggcgcgaaat 
     4081 caccgaccag ttgcatcagg taatgagcga ggtcgcactg ttgcgccaag cggtagagag 
     4141 cgaggtcagc agagtatcgg ccgacaaggc gctggcggat ggcctggtga agcggttcgg 
     4201 ggcggatgcg gaaaagtacc tgggcagaca gcctggtggc atccacagtg acgccgaagt 
     4261 gatggcgctt ggtctctaca ccggcattca ctacgcggac ctgaatcgcg ctctgcgtca 
     4321 ggggcaggag ctggatgcgg gacaaaagct gatcgaccaa ggtatgtccg cggccttcga 
     4381 gaagagcgga caggctgaac aggtagtgaa gactttccgt ggcacccgtg gcggggatgc 
     4441 cttcaacgcc gtggaagagg gcaaggttgg ccacgacgac ggctatctct ccacctccct 
     4501 gaaccccggt gtcgcgagga gcttcgggca gggcacgata tccaccgtgt tcggcaggtc 
     4561 cggaatcgat gtcagcggga tatcgaacta caagaatgaa aaagagattc tctataacaa 
     4621 agagaccgac atgcgcgtgc tgctgagcgc cagcgatgag cagggagtga cccgccgggt 
     4681 cctcgaagag gcggccctgg gggagcagag tggccatagc cagggactgc tcgatgctct 
     4741 cgacctggca agcaaaccgg aacgttcagg cgaggtccag gaacaggatg tacgcctgag 
     4801 gatgcgcggc cttgatctgg cccatcacca tcaccatcac tgaggatccc ccgggctgca 
     4861 ggaattcgat atcaagctta tcgataccgt cgacctcgag ggggggcccg gtacccagct 
     4921 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc 
     4981 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 
     5041 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 
     5101 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 
     5161 ggagaggcgg tttgcgtatt gggcgcatgc ataaaaactg ttgtaattca ttaagcattc 
     5221 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca 
     5281 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca 
     5341 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa 
     5401 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat 
     5461 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg 
     5521 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca 
     5581 ccagctcacc gtctttcatt gccatacgga attccggatg agcattcatc aggcgggcaa 
     5641 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg 
     5701 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct 
     5761 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt 
     5821 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta 
     5881 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt 
     5941 ttcgccaaaa gttggcccag ggcttcccgg tatcaacagg gacaccagga tttatttatt 
     6001 ctgcgaagtg atcttccgtc acaggtattt attcgaagac gaaagggcct cgtgatacgc 
     6061 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt 
     6121 cggggaaatg tgcgcgcccg cgttcctgct ggcgctgggc ctgtttctgg cgctggactt 
     6181 cccgctgttc cgtcagcagc ttttcgccca cggccttgat gatcgcggcg gccttggcct 
     6241 gcatatcccg attcaacggc cccagggcgt ccagaacggg cttcaggcgc tcccgaaggt 
//
