LOCUS       pCAGGS-FLAG-TAX1BP1-L        7066 bp    DNA     circular     17-JUL-2014
DEFINITION  LMBP 5163.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5163
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 17-JUL-2014
COMMENT     VNTDATE|438011151|
COMMENT     VNTNAME|pCAGGS-FLAG-TAX1BP1-L|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-FLAG-TAX1BP1-L" 0 7066 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "EcoRI" "SpeI" "XbaI" "XhoI" "XmnI" "BglII" "KpnI" "SalI" 
COMMENT         "ScaI") (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 
COMMENT        0 (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 7066 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 7065 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "SpeI" "actagt" 2 0 2 2286 0 5354 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "XbaI" "tctaga" 2 0 2 1825 0 6969 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 5 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 3 713 0 1131 0 5012 0 0 
COMMENT            "") 
COMMENT        #5=(CRSite (CStringList) "BglII" "agatct" 2 0 2 1354 0 2352 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 42 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "SalI" "gtcgac" 2 0 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "ScaI" "agtact" 4 0 2 220 0 4893 0 0 "")) 
COMMENT       (CObList 
COMMENT        #9=(CFSignal (CObList) "FLAG epitope" 22 0 0 13 36 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "13..36") 
COMMENT        #10=(CFSignal (CObList) "intron AG promoter" 15 0 0 6091 7015 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "6091..7015") 
COMMENT        #11=(CFSignal (CObList) "AG promoter" 29 0 0 5722 7063 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5722..7063") 
COMMENT        #12=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 5339 5716 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5339..5716") 
COMMENT        #13=(CFSignal (CObList) "ampicillin resistance" 4 0 1 4339 5199 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(4339..5199)") 
COMMENT        #14=(CFSignal (CObList) "pMB1 ori" 33 0 1 3397 3798 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3397..3798)") 
COMMENT        #15=(CFSignal (CObList) "SV40 polyA late" 43 0 1 3208 3282 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3208..3282)") 
COMMENT        #16=(CFSignal (CObList) "SV40 polyA early" 43 0 0 3208 3282 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3208..3282") 
COMMENT        #17=(CFSignal (CObList) "SV40 ori" 33 0 0 3102 3177 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3102..3177") 
COMMENT        #18=(CFSignal (CObList) "lac promoter" 30 0 1 2878 2960 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2878..2960)") 
COMMENT        #19=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 
COMMENT             0 2444 2800 0 (CStringList) (CStringList) 1 1 1 1 "2444..2800") 
COMMENT        #20=(CFSignal (CObList) "3' UTR of rabbit \337-globin, incl. polyA" 43 0 
COMMENT             0 2352 2443 0 (CStringList) (CStringList) 1 1 1 1 "2352..2443") 
COMMENT        #21=(CFSignal (CObList) "hTAX1BP1-L" 4 0 0 46 2289 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "46..2289")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList 
COMMENT        #22=(CFSignal (CObList) "LMBP 5163" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #23=(CFSignal (CObList) "Last modified: 17-JUL-2014" 2 0 0 0 0 0 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: margotbaetens" 
COMMENT                          1) 
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COMMENT                          "Created: 02/13/07 01:45" 1) 
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COMMENT                          "Last Modified: 07/17/14 11:26" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7066 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "Modification Date in the Original DB: 16-JUL-2014" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "EMAIL: <a href=\"mailto:margot.baetens@dmbr.ugent.be\">margot.baetens@dmbr.ugent.be</a>" 
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COMMENT                                        "   complement(4339..5199)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (2 total)") 
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COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                        "   complement(2878..2960)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT              (LOGPEN 0 0 0) 3.45846e-323 4.51821e-307) 
COMMENT        #334=(CSampFrag "pCAGGS-FLAG-TAX1BP1-L" 694 1131 "XmnI" 1825 "XbaI" 
COMMENT              (LOGPEN 0 0 0) 1.36576e-316 4.2373e-310) 
COMMENT        #335=(CSampFrag "pCAGGS-FLAG-TAX1BP1-L" 418 713 "XmnI" 1131 "XmnI" 
COMMENT              (LOGPEN 0 0 0) 1.39974e-316 4.51821e-307))))
FEATURES             Location/Qualifiers
     misc_marker     13..36
                     /vntifkey="22"
                     /label=FLAG\epitope
     intron          6091..7015
                     /vntifkey="15"
                     /label=intron\AG\promoter
     promoter        5722..7063
                     /vntifkey="29"
                     /label=AG\promoter
     promoter        5339..5716
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     CDS             complement(4339..5199)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(3397..3798)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(3208..3282)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      3208..3282
                     /vntifkey="43"
                     /label=SV40\polyA\early
     rep_origin      3102..3177
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(2878..2960)
                     /vntifkey="30"
                     /label=lac\promoter
     misc_feature    2444..2800
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     terminator      2352..2443
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     CDS             46..2289
                     /vntifkey="4"
                     /label=hTAX1BP1-L
BASE COUNT     1844 a      1666 c      1826 g      1730 t 
ORIGIN
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatgac atcctttcaa 
       61 gaagtcccat tgcagacttc caactttgcc catgtcatct ttcaaaatgt ggccaagagt 
      121 taccttccta atgcacacct ggaatgtcat tacaccttaa ctccatatat tcatccacat 
      181 ccaaaagatt gggttggtat attcaaggtt ggatggagta ctgctcgtga ttattacacg 
      241 tttttatggt cccctatgcc tgaacattat gtggaaggat caacagtcaa ttgtgtacta 
      301 gcattccaag gatattacct tccaaatgat gatggagaat tttatcagtt ctgttacgtt 
      361 acccataagg gtgaaattcg tggagcaagt acacctttcc agtttcgagc ttcttctcca 
      421 gttgaagagc tgcttactat ggaagatgaa ggaaattctg acatgttagt ggtgaccaca 
      481 aaagcaggcc ttcttgagtt gaaaattgag aaaaccatga aagaaaaaga agaactgtta 
      541 aagttaattg ccgttctgga aaaagaaaca gcacaacttc gagaacaagt tgggagaatg 
      601 gaaagagaac ttaaccatga gaaagaaaga tgtgaccaac tgcaagcaga acaaaagggt 
      661 cttactgaag taacacaaag cttaaaaatg gaaaatgaag agtttaagaa gaggttcagt 
      721 gatgctacat ccaaagccca tcagcttgag gaagatattg tgtcagtaac acataaagca 
      781 attgaaaaag aaaccgaatt agacagttta aaggacaaac tcaagaaggc acaacatgaa 
      841 agagaacaac ttgaatgtca gttgaagaca gagaaggatg aaaaggaact ttataaggta 
      901 catttgaaga atacagaaat agaaaatacc aagcttatgt cagaggtcca gactttaaaa 
      961 aatatagatg ggaacaaaga aagcgtgatt actcatttca aagaagagat tggcaggctg 
     1021 cagttatgtt tggctgaaaa ggaaaatctg caaagaactt tcctgcttac aacctcaagt 
     1081 aaagaagata cttgtttttt aaaggagcaa cttcgtaaag cagaggaaca ggttcaggca 
     1141 actcggcaag aagttgtctt tctggctaaa gaactcagtg atgctgtcaa cgtacgagac 
     1201 agaacgatgg cagacctgca tactgcacgc ttggaaaacg agaaagtgaa aaagcagtta 
     1261 gctgatgcag tggcagaact taaactaaat gctatgaaaa aagatcagga caagactgat 
     1321 acactggaac acgaactaag aagagaagtt gaagatctga aactccgtct tcagatggct 
     1381 gcagaccatt ataaagaaaa atttaaggaa tgccaaaggc tccaaaaaca aataaacaaa 
     1441 ctttcagatc aatcagctaa taataataat gtcttcacaa agaaaacggg gaatcagcag 
     1501 aaagtgaatg atgcttcagt aaacacagac ccagccactt ctgcctctac tgtagatgta 
     1561 aagccatcac cttctgcagc agaggcagat tttgacatag taacaaaggg gcaagtctgt 
     1621 gaaatgacca aagaaattgc tgacaaaaca gaaaagtata ataaatgtaa acaactcttg 
     1681 caggatgaga aagcaaaatg caataaatat gctgatgaac ttgcaaaaat ggagctgaaa 
     1741 tggaaagaac aagtgaaaat tgctgaaaat gtaaaacttg aactagctga agtacaggac 
     1801 aattataaag aacttaaaag gagtctagaa aatccagcag aaaggaaaat ggaagatgga 
     1861 gcagatggtg ctttttaccc agatgaaata caaaggccac ctgtcagagt cccctcttgg 
     1921 ggactggaag acaatgttgt ctgcagccag cctgctcgaa actttagtcg gcctgatggc 
     1981 ttagaggact ctgaggatag caaagaagat gagaatgtgc ctactgctcc tgatcctcca 
     2041 agtcaacatt tacgtgggca tgggacaggc ttttgctttg attccagctt tgatgttcac 
     2101 aagaagtgtc ccctctgtga gttaatgttt cctcctaact atgatcagag caaatttgaa 
     2161 gaacatgttg aaagtcactg gaaggtgtgc ccgatgtgca gcgagcagtt ccctcctgac 
     2221 tatgaccagc aggtgtttga aaggcatgtg cagacccatt ttgatcagaa tgttctaaat 
     2281 tttgactagt tactttttat tatgagttaa tatagtttag cagtaaaaaa aaaaaaaaaa 
     2341 aactcgacga agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg 
     2401 agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt 
     2461 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg 
     2521 agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt 
     2581 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata 
     2641 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt 
     2701 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac 
     2761 tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc 
     2821 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 
     2881 cacaacatac gagccggaag cataaagtgt aaagcctggg tgcctaatga gtgagctaac 
     2941 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 
     3001 ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 
     3061 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 
     3121 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 
     3181 tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa 
     3241 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 
     3301 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg 
     3361 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 
     3421 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 
     3481 tacggttatc cacagaatca gggataacgc aggaaagaac atgtgagcaa aaggccagca 
     3541 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 
     3601 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 
     3661 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 
     3721 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 
     3781 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 
     3841 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 
     3901 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 
     3961 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 
     4021 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 
     4081 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 
     4141 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 
     4201 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 
     4261 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 
     4321 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 
     4381 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 
     4441 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 
     4501 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 
     4561 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 
     4621 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 
     4681 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 
     4741 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 
     4801 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 
     4861 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 
     4921 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 
     4981 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 
     5041 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 
     5101 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 
     5161 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 
     5221 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 
     5281 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ggtcgatcga 
     5341 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca 
     5401 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac 
     5461 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact 
     5521 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa 
     5581 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg 
     5641 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta 
     5701 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc 
     5761 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg 
     5821 ggggcggggg gggggggggg gcccccccca ggcggggcgg ggcggggcga ggggcggggc 
     5881 ggggcgaggc ggaaaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct 
     5941 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga 
     6001 gtcgttgcgc gctgccttcc ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc 
     6061 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg 
     6121 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc 
     6181 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg 
     6241 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc 
     6301 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc 
     6361 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc 
     6421 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc 
     6481 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc 
     6541 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg 
     6601 ccgcctcggg ccggggaggg ctcgggggaa ggggcgcggc ggcccccgga gcgccggcgg 
     6661 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca 
     6721 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc 
     6781 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg 
     6841 ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg 
     6901 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac 
     6961 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct 
     7021 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaa 
//
