LOCUS       pXP2d2-promABIN-3        7181 bp    DNA     circular     14-JUN-2011
DEFINITION  LMBP 5175.
SOURCE      
  ORGANISM  
COMMENT     Accession number: 5175
            Last modified: 14-JUN-2011
            
            
            
COMMENT     VNTDATE|537031198|
COMMENT     VNTNAME|pXP2d2-promABIN-3|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pXP2d2-promABIN-3" 0 7181 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 1 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BglII" "SalI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 7181 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BglII" "agatct" 2 0 2 605 0 2162 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 26 0 0 "")) 
COMMENT       (CObList 
COMMENT        #2=(CFSignal (CObList) "SV40 polyA early" 43 0 0 4166 4262 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "4165..4261") 
COMMENT        #3=(CFSignal (CObList) "SV40 polyA late" 43 0 1 4166 4262 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(4165..4261)") 
COMMENT        #4=(CFSignal (CObList) "LUC" 4 0 0 2196 3848 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2195..3847") 
COMMENT        #5=(CFSignal (CObList) "SV40 polyA early" 43 0 0 7030 7126 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "7029..7125") 
COMMENT        #6=(CFSignal (CObList) "SV40 polyA early" 43 0 0 6793 6889 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "6792..6888") 
COMMENT        #7=(CFSignal (CObList) "SV40 polyA late" 43 0 1 7030 7126 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(7029..7125)") 
COMMENT        #8=(CFSignal (CObList) "SV40 polyA late" 43 0 1 6793 6889 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(6792..6888)") 
COMMENT        #9=(CFSignal (CObList) "pMB1 ori" 33 0 0 6021 6423 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "6020..6422") 
COMMENT        #10=(CFSignal (CObList) "ampicillin resistance" 4 0 0 4620 5480 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4619..5479") 
COMMENT        #11=(CFSignal (CObList) "hABIN-3 promoter" 29 0 0 31 2126 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "31..2125") 
COMMENT        #12=(CFSignal (CObList) "5' UTR of hABIN-3" 21 0 0 2127 2160 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2126..2159") 
COMMENT        #13=(CFSignal (CObList) "3' UTR of SV40 small t-antigen" 21 0 0 4046 
COMMENT             4165 0 (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #14=(CFSignal (CObList) "part of SV40 small t-antigen" 4 0 0 3904 3978 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3904..3978") 
COMMENT        #15=(CFSignal (CObList) "intron SV40 small t-antigen" 15 0 0 3979 4045 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #16=(CFSignal (CObList) "3' UTR of LUC" 21 0 0 3849 3893 0 (CStringList) 
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COMMENT        #17=(CFSignal (CObList) "Accession N\260.: 5175" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "") 
COMMENT        #18=(CFSignal (CObList) "Last modified: 14-JUN-2011" 2 0 0 0 0 0 
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COMMENT                    "pXP2d2-promABIN-3" 2) 5 "" 0 4) 
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COMMENT                          "DNA Plasmid 'pXP2d2-promABIN-3'" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Coll. N\260.: 5175." 1) 
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COMMENT                          "Local object" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 12/07/09 03:19" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 06/14/11 01:09" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7181 bp" 1) 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 7-DEC-2009" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                 "" 1))) "Accession N\260.: 5175") 
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COMMENT                                 "part of SV40 small t-antigen") 
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COMMENT                                 "ampicillin resistance"))) "CDS (3 total)") 
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COMMENT                          "Intron (1 total)") 
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COMMENT                                 "3' UTR of SV40 small t-antigen"))) 
COMMENT                          "Misc. Feature (3 total)") 
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COMMENT                                   #69=(CLinePar 
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COMMENT                          "Promoter Eukaryotic (1 total)") 
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COMMENT                          "Replication Origin (1 total)") 
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COMMENT                    "Restriction/Methylation Map") 
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COMMENT                    "Motifs (both strands)")))) 
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FEATURES             Location/Qualifiers
     CDS             3904..3978
                     /vntifkey="4"
                     /label=part\of\SV40\small\t-antigen
     terminator      4166..4262
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4166..4262)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     CDS             2196..3848
                     /vntifkey="4"
                     /label=LUC
     terminator      7030..7126
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      6793..6889
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(7030..7126)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      complement(6793..6889)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      6021..6423
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             4620..5480
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        31..2126
                     /vntifkey="29"
                     /label=hABIN-3\promoter
     misc_feature    2127..2160
                     /vntifkey="21"
                     /label=5'\UTR\of\hABIN-3
     misc_feature    4046..4165
                     /vntifkey="21"
                     /label=3'\UTR\of\SV40\small\t-antigen
     intron          3979..4045
                     /vntifkey="15"
                     /label=intron\SV40\small\t-antigen
     misc_feature    3849..3893
                     /vntifkey="21"
                     /label=3'\UTR\of\LUC
BASE COUNT     2066 a      1543 c      1567 g      2005 t 
ORIGIN
        1 ggatccaagc tcagatccaa gcttgtcgac gatggaggtg aagtgaacag agcatatgta 
       61 acagaaaaga cccaattaat tcttagggat ggcatattga aagtctcatt tttctgaaac 
      121 caaacttgtg gctcttcaga agagttatta cttattcata aataaccata tggctctctt 
      181 gagctaatag aaaatctgtt tgttgtttat ctgcataaca gaagaggcaa aggaattgta 
      241 aagaaagaag caaaggtata gaatttccat ttaatagaag ccatcacacc taacaatgta 
      301 tgtgcatcag gatattttac aggggagact caagaggcac aatgccaagg atggtatttc 
      361 ctggcaagaa aatgttcttc ccacccactt ttctaggtca atgttaccac atccctatac 
      421 aaatacaaag ttaaagctga agagaacctt acactatata aaactatgga gtgtgtatgt 
      481 gcatggtatg tttcagaaga aaaatattct tacaatagag ttttcttttt cttttctgat 
      541 gagcagattt tagtgttgat aaagtgtcag ttcaggaaat cgctgcttct gtaccctaaa 
      601 gtcagatctt atgtgagtta taacttttta ctttcagaac cctgaatgaa caatttgatt 
      661 cctacctgta tttttggatt ttgccttaaa gcagcgtcac tccctttcac tcaagcgtag 
      721 acccaaacat aacaagaaac cagaagaaag taaacacaca cacacacaca cacacacaca 
      781 cacacaaact agcaaaaagt tcatcgattt tgtaaatcat tatatagata ttcattcatt 
      841 tattcatgta gcaaaatgtg ttgaatgcct gctgtgtgcc agacagctca gtgcctgggt 
      901 gcgtggacac agagattaac acaagaacat ggaaatatgt aatcgtaatg catgatgaag 
      961 tgctctcctt agctgattag aggaagtcaa gagaagatca gaaggaaaga gaggcattga 
     1021 cttaggtttt ccccgtcata aggttggatg gagggcccag agaggagaca tctgaggtgg 
     1081 caagaacagc atgcacagag ctcaggggcg tagaaccatc tcactcattt caggaaaggg 
     1141 aggtcagtca ggatggttgg agcacagggg agagttggag agtaggggga gatattcagg 
     1201 agaaatggga gggccaaatg tgggacactc aatcactctc ttttgaattt taggagtttt 
     1261 aggctttatc ctatatatat gtgaatatcc cgtaggcagg ttaatgaagt taaatgattg 
     1321 accataaatt cttttctctt tttagttaca cattctttcc ttttttgtat tccttgcctc 
     1381 tttccttttg ttccaagtcc aaaatgaggt cttctgttcc atcgcagttc acttatgcag 
     1441 ctccaagtat ctgccatttc cacgctttta tctgtactac ccagtagtgc tccattacct 
     1501 caaggctcct aaaatgataa cagtcaaata attaaagtga aaatgattct ccatgagaag 
     1561 atttcacttc aaatgagtca tggagacaat ctggacttga tttatagaaa agacagactt 
     1621 aaacttgaga caagtttaac ctttctgcca atatagaaga ttagatttgc aaacaatgct 
     1681 cattttcttt acaatgaatt attttccctt ttatttctat aagaattagt ggcacttata 
     1741 ttattatgta ttatgaatta tgtatgactc attaagttct accaagctac actcacattt 
     1801 agaaggtatt gccagaaaat ctcgccttta gggaaaccga gctgtcaaaa agtcaacagt 
     1861 tacctaattg ctctgggaaa tacaatgaat ttataacagt taggctactg aggaaacact 
     1921 tggattgccc aggctttctg tggtggagaa cttcctcctc cagtgggaga aactgccagt 
     1981 aaaggaagaa tgacgattta catactatag caaggctaat ctgccccagg tcttagtcac 
     2041 ggcctgccta tttgagacat cctgggaaat ccctatttgg aagagaagaa aagttaaaat 
     2101 cccagttgca gtgcttataa ctttattctg tgtaattcct atccacagtc acttctgttc 
     2161 agatctgagc ttggcattcc ggtactgttg gtaaaatgga agacgccaaa aacataaaga 
     2221 aaggcccggc gccattctat cctctagagg atggaaccgc tggagagcaa ctgcataagg 
     2281 ctatgaagag atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg 
     2341 tgaacatcac gtacgcggaa tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac 
     2401 gatatgggct gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct 
     2461 ttatgccggt gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt 
     2521 ataatgaacg tgaattgctc aacagtatga acatttcgca gcctaccgta gtgtttgttt 
     2581 ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa attaccaata atccagaaaa 
     2641 ttattatcat ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca 
     2701 catctcatct acctcccggt tttaatgaat acgattttgt accagagtcc tttgatcgtg 
     2761 acaaaacaat tgcactgata atgaattcct ctggatctac tgggttacct aagggtgtgg 
     2821 cccttccgca tagaactgcc tgcgtcagat tctcgcatgc cagagatcct atttttggca 
     2881 atcaaatcat tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa 
     2941 tgtttactac actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg 
     3001 aagaagagct gtttttacga tcccttcagg attacaaaat tcaaagtgcg ttgctagtac 
     3061 caaccctatt ttcattcttc gccaaaagca ctctgattga caaatacgat ttatctaatt 
     3121 tacacgaaat tgcttctggg ggcgcacctc tttcgaaaga agtcggggaa gcggttgcaa 
     3181 aacgcttcca tcttccaggg atacgacaag gatatgggct cactgagact acatcagcta 
     3241 ttctgattac acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt 
     3301 ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct gggcgttaat cagagaggcg 
     3361 aattatgtgt cagaggacct atgattatgt ccggttatgt aaacaatccg gaagcgacca 
     3421 acgccttgat tgacaaggat ggatggctac attctggaga catagcttac tgggacgaag 
     3481 acgaacactt cttcatagtt gaccgcttga agtctttaat taaatacaaa ggatatcagg 
     3541 tggcccccgc tgaattggaa tcgatattgt tacaacaccc caacatcttc gacgcgggcg 
     3601 tggcaggtct tcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc 
     3661 acggaaagac gatgacggaa aaagagatcg tggattacgt cgccagtcaa gtaacaaccg 
     3721 cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa 
     3781 aactcgacgc aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagtcca 
     3841 aattgtaaaa tgtaactgta ttcagcgatg acgaaattct tagctattgt aatgactcta 
     3901 gaggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac 
     3961 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact 
     4021 gattctaatt gtttgtgtat tttagattcc aacctatttg atgtatagtg ccttgactag 
     4081 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac 
     4141 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg 
     4201 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 
     4261 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga 
     4321 tcgatcccca ggaagctcct ctgtgtcctc ataaacccta acctcctcta cttgagagga 
     4381 cattccaatc ataggctgcc catccaccct ctgtgtcctc ctgttaatta ggtcacttaa 
     4441 caaaaaggaa attgggtagg ggtttttcac agaccgcttt ctaagggtaa ttttaaaata 
     4501 tctgggaagt cccttccact gctgtgttcc agaagtgttg gtaaacagcc cacaaatgtc 
     4561 aacagcagaa acatacaagc tgtcagcttt gcacaaggga atattgaaaa aggaagagta 
     4621 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 
     4681 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 
     4741 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 
     4801 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 
     4861 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 
     4921 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 
     4981 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 
     5041 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 
     5101 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 
     5161 ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 
     5221 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 
     5281 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 
     5341 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 
     5401 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 
     5461 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt 
     5521 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 
     5581 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 
     5641 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 
     5701 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 
     5761 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 
     5821 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 
     5881 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 
     5941 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 
     6001 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 
     6061 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 
     6121 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 
     6181 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 
     6241 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 
     6301 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg 
     6361 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag 
     6421 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt 
     6481 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc 
     6541 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg 
     6601 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg 
     6661 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggga tcataatcag 
     6721 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa 
     6781 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg 
     6841 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 
     6901 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca taatcagcca 
     6961 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct 
     7021 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta 
     7081 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 
     7141 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc t 
//
