ID pCAGGS-E-hPELI2 standard; circular DNA; ; 6124 BP. DE LMBP 5197. KW Reinout. CC Accession number: LMBP 5197 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 29-OCT-2009 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_marker 4816..4854 FT /vntifkey="22" FT /label=E-tag FT /note="E-tag" FT terminator 90..181 FT /vntifkey="43" FT /label=3'\UTR\of\ß-globin,\incl.\polyA FT /note="3' UTR of the rabbit ß-globin gene, incl. polyA" FT misc_feature 182..538 FT /vntifkey="21" FT /label=3'\flanking\region\of\rabbit\ß-globin FT /note="3' flanking region of the rabbit ß-globin gene" FT promoter complement(616..698) FT /vntifkey="30" FT /label=lac\promoter FT /note="E. coli lac operon promoter" FT rep_origin 840..915 FT /vntifkey="33" FT /label=SV40\ori FT /note="SV40 fragment containing bidirectional ori" FT terminator 946..1020 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(946..1020) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT rep_origin complement(1135..1536) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(2077..2937) FT /vntifkey="4" FT /label=ampicillin\resistance FT /note="antibiotic resistance gene" FT promoter 3073..3450 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT /note="hCMV-IE enhancer" FT promoter 3456..4797 FT /vntifkey="29" FT /label=AG\promoter FT /note="ß-actin/ß-globin hybrid promoter" FT CDS 5866..6063 FT /vntifkey="4" FT /label=RING-domain FT CDS 4864..2 FT /vntifkey="4" FT /label=hPELI2 FT intron 3825..4749 FT /vntifkey="15" FT /label=intron\AG\promoter SQ Sequence 6124 BP; 1330 A; 1629 C; 1709 G; 1456 t; gactcgagga attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg 60 ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg 120 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg 180 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc 240 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg 300 ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg 360 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt 420 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat 480 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc 540 tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 600 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctgggtg 660 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 720 gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct 780 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 840 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 900 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca 960 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 1020 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc 1080 cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 1140 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 1200 ctcactcaaa ggcggtaata cggttatcca cagaatcagg gataacgcag gaaagaacat 1260 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 1320 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 1380 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 1440 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 1500 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 1560 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 1620 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 1680 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 1740 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 1800 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 1860 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 1920 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 1980 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 2040 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 2100 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 2160 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 2220 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 2280 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 2340 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 2400 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 2460 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 2520 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 2580 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 2640 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 2700 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 2760 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 2820 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 2880 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 2940 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 3000 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 3060 gccacctggg tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat 3120 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg 3180 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa 3240 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact 3300 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta 3360 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt 3420 acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca 3480 ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat 3540 tttgtgcagc gatgggggcg gggggggggg gggggccccc cccaggcggg gcggggcggg 3600 gcgaggggcg gggcggggcg aggcggaaag gtgcggcggc agccaatcag agcggcgcgc 3660 tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg 3720 cgcggcgggc gggagtcgtt gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct 3780 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg 3840 gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt 3900 ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg 3960 ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg 4020 gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga 4080 gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg 4140 tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac 4200 cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc 4260 gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc 4320 cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaaggggcg cggcggcccc 4380 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg 4440 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg 4500 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa 4560 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct 4620 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc 4680 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt 4740 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa 4800 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 4860 gcaatgtttt cccctggcca ggaggaacac tgcgccccca ataaggagcc agtgaaatac 4920 ggggagctgg tggtgctcgg gtacaatggt gctttaccca atggagatag aggacggagg 4980 aaaagtagat ttgccctcta caagcggccc aaggcaaatg gtgtcaaacc cagcaccgtc 5040 catgtgatat ccacgcccca ggcatccaag gctatcagct gcaaaggtca acacagtata 5100 tcctacactt tgtcaaggaa tcagactgtg gtggtggagt acacacatga taaggatacg 5160 gatatgtttc aggtgggcag atcaacagaa agccctatcg acttcgttgt cacagacacg 5220 atttctggca gccagaacac ggacgaagcc cagatcacac agagcaccat atccaggttc 5280 gcctgcagga tcgtgtgcga caggaatgaa ccttacacag cacggatatt cgccgccgga 5340 tttgactctt ccaaaaacat atttcttgga gaaaaggcag caaagtggaa aaaccccgac 5400 ggccacatgg atgggctcac tactaatggc gtcctggtga tgcatccacg agggggcttc 5460 accgaggagt cccagcccgg ggtctggcgc gagatctctg tctgtggaga tgtgtacacc 5520 ttgcgagaaa ccaggtcggc ccagcaacga ggaaagctgg tggaaagtga gaccaacgtc 5580 ctgcaggacg gctccctcat tgacctgtgt ggggccactc tcctctggag aacagcagat 5640 gggctttttc atactccaac tcagaagcac atagaagccc tccggcagga gattaacgcc 5700 gcccggcctc agtgtcctgt ggggctcaac accctggcct tccccagcat caacaggaaa 5760 gaggtggtgg aggagaagca gccctgggca tatctcagtt gtggccacgt gcacgggtac 5820 cacaactggg gccatcggag tgacacggag gccaacgaga gggagtgtcc catgtgcagg 5880 actgtgggcc cctatgtgcc tctctggctt ggctgtgagg caggatttta tgtagacgca 5940 ggaccgccaa ctcatgcttt cactccctgt ggacacgtgt gctcggagaa gtctgcaaaa 6000 tactggtctc agatcccgtt gcctcatgga actcatgcat ttcacgctgc ttgccctttc 6060 tgtgctacac agctggttgg ggagcaaaac tgcatcaaat taattttcca aggtccaatt 6120 gact 6124 //