LOCUS       pCAGGS-E-hPELI3-PM        6277 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 5200.
SOURCE      
  ORGANISM  
COMMENT     This file is best viewed with a free Vector NTI Viewer:
            http://www.informaxinc.com/view/viewer.html
COMMENT     VNTNAME|pCAGGS-E-hPELI3-PM|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

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FEATURES             Location/Qualifiers
     CDS             4819..4857
                     /vntifkey="4"
                     /label=E-tag
                     /note="E-tag"
     terminator      93..184
                     /vntifkey="43"
                     /label=polyA
                     /note="3' UTR of the rabbit ç-globin gene, incl. polyA"
     misc_feature    185..541
                     /vntifkey="21"
                     /label=3FR
                     /note="3' flanking region of the rabbit ç-globin gene"
     promoter        complement(619..701)
                     /vntifkey="30"
                     /label=lac
                     /note="E. coli lac operon promoter"
     rep_origin      748..948
                     /vntifkey="33"
                     /label=SVORI
                     /note="SV40 fragment containing bidirectional ori"
     terminator      949..1023
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 early region"
     terminator      complement(949..1023)
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(1138..1539)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(2080..2940)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene"
     promoter        3076..3453
                     /vntifkey="30"
                     /label=hCMV
                     /note="hCMV-IE enhancer"
     promoter        3459..4803
                     /vntifkey="30"
                     /label=AG
                     /note="ç-actin/ç-globin hybrid promoter"
     CDS             4867..6276
                     /vntifkey="4"
                     /label=Pellino-3\CDS
     misc_feature    6016..6213
                     /vntifkey="21"
                     /label=RING-domain
     misc_feature    6124..6126
                     /vntifkey="21"
                     /label=Mut\H420S
     misc_feature    6130..6132
                     /vntifkey="21"
                     /label=Mut\C422S
     misc_feature    5524..5526
                     /vntifkey="21"
                     /label=Mut\A->V
BASE COUNT     1270 a      1761 c      1801 g      1445 t 
ORIGIN
        1 tcgaggtcga ggaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg 
       61 tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg 
      121 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca 
      181 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa 
      241 atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc 
      301 tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg 
      361 ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt 
      421 gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca 
      481 gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat 
      541 ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat 
      601 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 
      661 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 
      721 cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc 
      781 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 
      841 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 
      901 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt 
      961 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 
     1021 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 
     1081 atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 
     1141 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 
     1201 cagctcactc aaaggcggta atacggttat ccacagaatc agggataacg caggaaagaa 
     1261 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 
     1321 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 
     1381 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 
     1441 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 
     1501 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 
     1561 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 
     1621 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 
     1681 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 
     1741 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 
     1801 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 
     1861 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 
     1921 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 
     1981 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 
     2041 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 
     2101 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 
     2161 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 
     2221 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 
     2281 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 
     2341 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 
     2401 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 
     2461 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 
     2521 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 
     2581 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 
     2641 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 
     2701 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 
     2761 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 
     2821 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 
     2881 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 
     2941 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 
     3001 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 
     3061 agtgccacct gggtcgacat tgattattga ctagttatta atagtaatca attacggggt 
     3121 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc 
     3181 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag 
     3241 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc 
     3301 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg 
     3361 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc 
     3421 agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct 
     3481 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt attttttaat 
     3541 tattttgtgc agcgatgggg gcgggggggg ggggggggcc ccccccaggc ggggcggggc 
     3601 ggggcgaggg gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg 
     3661 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa 
     3721 gcgcgcggcg ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc tccgccgccg 
     3781 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg 
     3841 acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc 
     3901 tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct 
     3961 cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg 
     4021 gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg 
     4081 ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct 
     4141 gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc 
     4201 aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc 
     4261 tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg 
     4321 tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggaaggg gcgcggcggc 
     4381 ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa 
     4441 tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg 
     4501 aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag 
     4561 gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag 
     4621 cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc 
     4681 ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc 
     4741 tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa 
     4801 gaattgaatt ccaccatggg tgcgccggtg ccgtatccgg acccgctgga accgcgtgcg 
     4861 gccgcaatgg tgctggaagg aaaccctgaa gtggggtccc cccgaacctc agacctccag 
     4921 caccggggga acaagggctc ttgcgttctc tcctctcccg gtgaagatgc gcagccaggc 
     4981 gaggagccca tcaagtatgg tgaactcatc gtcctgggct gctgtgagga aggaggtgag 
     5041 gaaaccgagg ctcagagagg ggaagtgact ggcccaaggg cacacagctg ctacaatggt 
     5101 tgtctggcaa gtggggacaa gggccgccgg cgaagccgcc tggcactgag ccgccggtcg 
     5161 cacgccaacg gggtgaagcc agacgtcatg caccacatct ccacgccgct cgtctccaag 
     5221 gcactgagta accgtggtca gcacagcatc tcgtatacac tgtcccggag ccactcggtc 
     5281 atagtggagt atacacatga tagcgacaca gacatgttcc agattggccg ctccacagag 
     5341 aacatgattg acttcgtggt aacagacacg tcccctggag gaggggctgc cgagggccct 
     5401 tctgcccaga gcaccatctc ccgctatgcc tgccgcatcc tctgtgaccg ccggccaccc 
     5461 tatactgccc gcatctatgc cgctggcttc gatgcctcta gcaacatctt ccttggagag 
     5521 cgagcggcca aatggcggac cccagatggc ctgatggatg gactgaccac caatggagtc 
     5581 ctggtgatgc acccggcagg cggcttctcc gaggactcag ccccgggtgt ctggcgggag 
     5641 atctcggtct gtgggaatgt gtacacattg cgggacagcc gctcagccca gcagcggggc 
     5701 aagctggtgg aaaacgagtc caacgtgctg caggacggct ctctcatcga cctgtgtggg 
     5761 gccacactgc tgtggcgcac accggcgggg ctgctgcggg ctcccacact gaagcaactg 
     5821 gaggcccagc ggcaggaggc aaatgcagcg cggccccagt gccccgtggg cctcagcact 
     5881 ctggccttcc ccagcccagc ccgtggccgc acagcgcccg acaaacagca gccctgggtc 
     5941 tacgtccgct gcgggcacgt ccatggctac cacggctggg gctgccggcg ggagcggggc 
     6001 ccccaggagc gcgaatgtcc tctctgccgc cttgtggggc cttatgtgcc tctatggctt 
     6061 ggccaggagg ccggcctctg cctggaccct gggccgccta gccatgcctt tgcaccttgc 
     6121 ggctctgtct cctctgagaa gactgcccgc tactgggccc agacaccact gccccacggc 
     6181 acccatgctt tccatgccgc ctgccccttt tgcggggcct ggcttaccgg cgagcatggc 
     6241 tgcgtccgcc tcattttcca gggcccgctg gattagc 
//
