LOCUS       pCAGGS-deltaPTB         5340 bp    DNA     circular     24-OCT-2007
DEFINITION  LMBP 5215.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5215
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 24-OCT-2007
            
COMMENT     VNTDATE|436376515|
COMMENT     VNTNAME|pCAGGS-deltaPTB|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #2=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 4 7 0 172 0 1373 0 3989 0 0 
COMMENT            "")) 
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COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #6=(CFSignal (CObList) "lac promoter" 30 0 1 1156 1238 1 (CStringList) 
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COMMENT        #7=(CFSignal (CObList) "ampicillin" 4 0 1 2617 3477 1 (CStringList) 
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COMMENT        #8=(CFSignal (CObList) "intron AG promoter" 15 0 0 4365 5289 1 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
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FEATURES             Location/Qualifiers
     misc_feature    395..625
                     /vntifkey="21"
                     /label=RRM2
                     /note="RNA recognition motif 2"
     misc_feature    20..274
                     /vntifkey="21"
                     /label=RRM1
                     /note="RNA recognition motif 1"
     promoter        3613..3990
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     misc_feature    722..1078
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the ß-globin gene"
     terminator      complement(1486..1560)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     terminator      1486..1560
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     promoter        3996..5337
                     /vntifkey="29"
                     /label=AG\promoter
     rep_origin      complement(1675..2076)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      630..721
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     promoter        complement(1156..1238)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      1380..1455
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     CDS             complement(2617..3477)
                     /vntifkey="4"
                     /label=ampicillin
     intron          4365..5289
                     /vntifkey="15"
                     /label=intron\AG\promoter
     CDS             8..628
                     /vntifkey="4"
                     /label=deltaPTB
BASE COUNT     1117 a      1455 c      1488 g      1280 t 
ORIGIN
        1 ttccaccatg gtcccctcta gagtgatcca catccggaag ctccccatcg acgtcacgga 
       61 gggggaagtc atctccctgg ggctgccctt tgggaaggtc accaacctcc tgatgctgaa 
      121 ggggaaaaac caggccttca tcgagatgaa cacggaggag gctgccaaca ccatggtgaa 
      181 ctactacacc tcggtgaccc ctgtgctgcg cggccagccc atctacatcc agttctccaa 
      241 ccacaaggag ctgaagaccg acagctctcc caaccaggcg cgggcccagg cggccctgca 
      301 ggcggtgaac tcggtccagt cggggaacct ggccttggct gcctcggcgg cggccgtgga 
      361 cgcagggatg gcgatggccg ggcagagccc cgtgctcagg atcatcgtgg agaacctctt 
      421 ctaccctgtg accctggatg tgctgcacca gattttctcc aagttcggca cagtgttgaa 
      481 gatcatcacc ttcaccaaga acaaccagtt ccaggccctg ctgcagtatg cggaccccgt 
      541 gagcgcccag cacgccaagc tgtcgctgga cgggcagaac atctacaacg cctgctgcac 
      601 gctgcgcatc gacttttcca agctctagag atctttttcc ctctgccaaa aattatgggg 
      661 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg 
      721 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc 
      781 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg 
      841 ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg 
      901 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt 
      961 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat 
     1021 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc 
     1081 tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 
     1141 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctgggtg 
     1201 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 
     1261 gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct 
     1321 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 
     1381 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 
     1441 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca 
     1501 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 
     1561 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc 
     1621 cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 
     1681 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 
     1741 ctcactcaaa ggcggtaata cggttatcca cagaatcagg gataacgcag gaaagaacat 
     1801 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 
     1861 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 
     1921 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 
     1981 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 
     2041 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 
     2101 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 
     2161 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 
     2221 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 
     2281 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 
     2341 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 
     2401 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 
     2461 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 
     2521 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 
     2581 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 
     2641 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 
     2701 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 
     2761 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 
     2821 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 
     2881 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 
     2941 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 
     3001 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 
     3061 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 
     3121 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 
     3181 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 
     3241 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 
     3301 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 
     3361 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 
     3421 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 
     3481 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 
     3541 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 
     3601 gccacctggg tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat 
     3661 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg 
     3721 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa 
     3781 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact 
     3841 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta 
     3901 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt 
     3961 acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca 
     4021 ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat 
     4081 tttgtgcagc gatgggggcg gggggggggg gggggccccc cccaggcggg gcggggcggg 
     4141 gcgaggggcg gggcggggcg aggcggaaag gtgcggcggc agccaatcag agcggcgcgc 
     4201 tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg 
     4261 cgcggcgggc gggagtcgtt gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct 
     4321 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg 
     4381 gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt 
     4441 ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg 
     4501 ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg 
     4561 gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga 
     4621 gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg 
     4681 tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac 
     4741 cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc 
     4801 gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc 
     4861 cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaaggggcg cggcggcccc 
     4921 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg 
     4981 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg 
     5041 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa 
     5101 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct 
     5161 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc 
     5221 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt 
     5281 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa 
//
