LOCUS       pCAGGST7                4818 bp    DNA     circular     29-OCT-2007
DEFINITION  LMBP 5216.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5216
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 29-OCT-2007
            
COMMENT     VNTDATE|435580091|
COMMENT     VNTNAME|pCAGGST7|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGST7" 0 4818 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
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COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 21 22 62 29 30 32 33 43) 
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COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
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COMMENT       (CMolPar 0 0 0 4414 4408 1 4818 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 22 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 4 194 1 2230 0 2404 1 4438 
COMMENT            1 0 "") 
COMMENT        #2=(CRSite (CStringList) "Eco105I" "tacgta" 4 0 1 3447 0 0 "")) 
COMMENT       (CObList 
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COMMENT            (CStringList) (CStringList) 1 1 1 1 "3091..3468") 
COMMENT        #4=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 200 556 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "200..556") 
COMMENT        #5=(CFSignal (CObList) "SV40 polyA late" 43 0 1 964 1038 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(964..1038)") 
COMMENT        #6=(CFSignal (CObList) "SV40 polyA early" 43 0 0 964 1038 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "964..1038") 
COMMENT        #7=(CFSignal (CObList) "AG promoter" 29 0 0 3474 4815 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3474..4815") 
COMMENT        #8=(CFSignal (CObList) "pMB1 ori" 33 0 1 1153 1554 0 (CStringList) 
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COMMENT        #9=(CFSignal (CObList) "\337-globin polyA" 43 0 0 108 199 0 
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COMMENT        #10=(CFSignal (CObList) "lac promoter" 30 0 1 634 716 0 (CStringList) 
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COMMENT        #11=(CFSignal (CObList) "SV40 ori" 33 0 0 858 933 0 (CStringList) 
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COMMENT        #12=(CFSignal (CObList) "ampicillin" 4 0 1 2095 2955 0 (CStringList) 
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COMMENT        #13=(CFSignal (CObList) "intron AG promoter" 15 0 0 3843 4767 0 
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COMMENT                          "DNA Plasmid 'pCAGGST7'" 1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Created: 01/17/07 10:28" 1) 
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COMMENT                          "Last Modified: 10/29/07 09:41" 1) 
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COMMENT                          "length: 4818 bp" 1) 
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FEATURES             Location/Qualifiers
     promoter        3091..3468
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     misc_feature    200..556
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of ß-globin gene"
     terminator      complement(964..1038)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     terminator      964..1038
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     promoter        3474..4815
                     /vntifkey="29"
                     /label=AG\promoter
     rep_origin      complement(1153..1554)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      108..199
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     promoter        complement(634..716)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      858..933
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     CDS             complement(2095..2955)
                     /vntifkey="4"
                     /label=ampicillin
     intron          3843..4767
                     /vntifkey="15"
                     /label=intron\AG\promoter
     promoter        1..17
                     /vntifkey="30"
                     /label=T7\promoter
BASE COUNT     1010 a      1275 c      1341 g      1192 t 
ORIGIN
        1 taatacgact cactataggg ctcgaggaat tcactcctca ggtgcaggct gcctatcaga 
       61 aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct 
      121 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa 
      181 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga 
      241 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa 
      301 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata 
      361 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga 
      421 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac 
      481 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc 
      541 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg 
      601 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 
      661 aagtgtaaag cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 
      721 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc 
      781 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 
      841 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 
      901 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa 
      961 gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 
     1021 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 
     1081 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 
     1141 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 
     1201 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga 
     1261 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 
     1321 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 
     1381 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 
     1441 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 
     1501 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 
     1561 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 
     1621 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 
     1681 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 
     1741 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 
     1801 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 
     1861 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 
     1921 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 
     1981 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 
     2041 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 
     2101 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 
     2161 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 
     2221 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 
     2281 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 
     2341 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 
     2401 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 
     2461 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 
     2521 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 
     2581 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 
     2641 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 
     2701 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 
     2761 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 
     2821 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 
     2881 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 
     2941 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 
     3001 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 
     3061 cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt 
     3121 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta 
     3181 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga 
     3241 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt 
     3301 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta 
     3361 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg 
     3421 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga 
     3481 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt 
     3541 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc 
     3601 caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag 
     3661 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc 
     3721 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc 
     3781 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac 
     3841 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac 
     3901 ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt 
     3961 gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg 
     4021 gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc 
     4081 gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg 
     4141 aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg 
     4201 cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg 
     4261 cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt 
     4321 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg 
     4381 aaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat 
     4441 tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg 
     4501 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc 
     4561 ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc 
     4621 cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg 
     4681 ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat 
     4741 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct 
     4801 catcattttg gcaaagaa 
//
