LOCUS       pCAGGS-E-hTRIF          7025 bp    DNA     circular      5-SEP-2014
DEFINITION  LMBP 5226.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5226
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 05-SEP-2014
COMMENT     VNTDATE|429710341|
COMMENT     VNTNAME|pCAGGS-E-hTRIF|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-E-hTRIF" 0 7025 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BamHI" "EcoRI" "NotI" "XbaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 5970 5966 1 7025 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 4 511 0 2227 0 2965 0 3302 
COMMENT            0 0 "") #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 4 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XbaI" "tctaga" 2 0 2 241 0 6928 0 0 "")) 
COMMENT       (CObList 
COMMENT        #4=(CFSignal (CObList) "E-tag" 22 0 0 16 54 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "16..54") 
COMMENT        #5=(CFSignal (CObList) "intron AG promoter" 15 0 0 6050 6974 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "6050..6974") 
COMMENT        #6=(CFSignal (CObList) "hTRIF" 4 0 0 64 2199 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "64..2199") 
COMMENT        #7=(CFSignal (CObList) "3' UTR of rabbit \337-globin, incl. polyA" 43 0 
COMMENT            0 2315 2406 0 (CStringList) (CStringList) 1 1 1 1 "2315..2406") 
COMMENT        #8=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 0 
COMMENT            2407 2763 0 (CStringList) (CStringList) 1 1 1 1 "2407..2763") 
COMMENT        #9=(CFSignal (CObList) "lac promoter" 30 0 1 2841 2923 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2841..2923)") 
COMMENT        #10=(CFSignal (CObList) "SV40 ori" 33 0 0 3065 3140 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3065..3140") 
COMMENT        #11=(CFSignal (CObList) "SV40 polyA early" 43 0 0 3171 3245 0 
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COMMENT        #12=(CFSignal (CObList) "SV40 polyA late" 43 0 1 3171 3245 0 
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COMMENT        #13=(CFSignal (CObList) "pMB1 ori" 33 0 1 3360 3761 0 (CStringList) 
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COMMENT        #14=(CFSignal (CObList) "ampicillin resistance" 4 0 1 4302 5162 0 
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COMMENT        #15=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 5298 5675 0 
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COMMENT        #16=(CFSignal (CObList) "AG promoter" 29 0 0 5681 7022 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pCAGGS-E-hTRIF'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Created: 12/13/06 11:59" 1) 
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COMMENT                          "Last Modified: 09/05/14 12:40" 1) 
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COMMENT                          "Modification Date in the Original DB: 24-OCT-2007" 1))) 
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FEATURES             Location/Qualifiers
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
                     /note="E-tag"
     CDS             64..2199
                     /vntifkey="4"
                     /label=hTRIF
     terminator      2315..2406
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_feature    2407..2763
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     promoter        complement(2841..2923)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      3065..3140
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      3171..3245
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(3171..3245)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(3360..3761)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(4302..5162)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        5298..5675
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     promoter        5681..7022
                     /vntifkey="29"
                     /label=AG\promoter
     intron          6050..6974
                     /vntifkey="15"
                     /label=intron\AG\promoter
BASE COUNT     1430 a      2089 c      1945 g      1561 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gctgcctgca caggcccatc acttcctagc gccttcgaca ttctaggtgc agcaggccag 
      121 gacaagctct tgtatctgaa gcacaaactg aagaccccac gcccaggctg ccaggggcag 
      181 gacctcctgc atgccatggt tctcctgaag ctgggccagg aaactgaggc caggatctct 
      241 ctagaggcat tgaaggccga tgcggtggcc cggctggtgg cccgccagtg ggctggcgtg 
      301 gacagcaccg aggacccaga ggagccccca gatgtgtcct gggctgtggc ccgcttgtac 
      361 cacctgctgg ctgaggagaa gctgtgcccc gcctcgctgc gggacgtggc ctaccaggaa 
      421 gccgtccgca ccctcagctc cagggacgac caccggctgg gggaacttca ggatgaggcc 
      481 cgaaaccggt gtgggtggga cattgctggg gatccaggga gcatccggac gctccagtcc 
      541 aatctgggct gcctcccacc atcctcggct ttgccctctg ggaccaggag cctcccacgc 
      601 cccattgacg gtgtttcgga ctggagccaa gggtgctccc tgcgatccac tggcagccct 
      661 gcctccctgg ccagcaactt ggaaatcagc cagtccccta ccatgccctt cctcagcctg 
      721 caccgcagcc cacatgggcc cagcaagctc tgtgacgacc cccaggccag cttggtgccc 
      781 gagcctgtcc ccggtggctg ccaggagcct gaggagatga gctggccgcc atcgggggag 
      841 attgccagcc caccagagct gccaagcagc ccacctcctg ggcttcccga agtggcccca 
      901 gatgcaacct ccactggcct ccctgatacc cccgcagctc cagaaaccag caccaactac 
      961 ccagtggagt gcaccgaggg gtctgcaggc ccccagtctc tccccttgcc tattctggag 
     1021 ccggtcaaaa acccctgctc tgtcaaagac cagacgccac tccaactttc tgtagaagat 
     1081 accacctctc caaataccaa gccgtgccca cctactccca ccaccccaga aacatcccct 
     1141 cctcctcctc ctcctcctcc ttcatctact ccttgttcag ctcacctgac cccctcctcc 
     1201 ctgttccctt cctccctgga atcatcatcg gaacagaaat tctataactt tgtgatcctc 
     1261 cacgccaggg cagacgaaca catcgccctg cgggttcggg agaagctgga ggcccttggc 
     1321 gtgcccgacg gggccacctt ctgcgaggat ttccaggtgc cggggcgcgg ggagctgagc 
     1381 tgcctgcagg acgccataga ccactcagct ttcatcatcc tacttctcac ctccaacttc 
     1441 gactgtcgcc tgagcctgca ccaggtgaac caagccatga tgagcaacct cacgcgacag 
     1501 gggtcgccag actgtgtcat ccccttcctg cccctggaga gctccccggc ccagctcagc 
     1561 tccgacacgg ccagcctgct ctccgggctg gtgcggctgg acgaacactc ccagatcttc 
     1621 gccaggaagg tggccaacac cttcaagccc cacaggcttc aggcccgaaa ggccatgtgg 
     1681 aggaaggaac aggacacccg agccctgcgg gaacagagcc aacacctgga cggtgagcgg 
     1741 atgcaggcgg cggcactgaa cgcagcctac tcagcctacc tccagagcta cttgtcctac 
     1801 caggcacaga tggagcagct ccaggtggct tttgggagcc acatgtcatt tgggactggg 
     1861 gcgccctatg gggctcgaat gccctttggg ggccaggtgc ccctgggagc cccgccaccc 
     1921 tttcccactt ggccggggtg cccgcagccg ccacccctgc acgcatggca ggctggcacc 
     1981 cccccaccgc cctccccaca gccagcagcc tttccacagt cactgccctt cccgcagtcc 
     2041 ccagccttcc ctacggcctc acccgcaccc cctcagagcc cagggctgca acccctcatt 
     2101 atccaccacg cacagatggt acagctgggg ctgaacaacc acatgtggaa ccagagaggg 
     2161 tcccaggcgc ccgaggacaa gacgcaggag gcagaatgac ccgggacatt aaacgcgtgg 
     2221 gtaccggatc cggcaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg 
     2281 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta 
     2341 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt 
     2401 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc 
     2461 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat 
     2521 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc 
     2581 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt 
     2641 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc 
     2701 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag 
     2761 atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 
     2821 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 
     2881 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 
     2941 gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg 
     3001 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 
     3061 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 
     3121 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta 
     3181 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 
     3241 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 
     3301 ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 
     3361 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 
     3421 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcagggataa cgcaggaaag 
     3481 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 
     3541 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 
     3601 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 
     3661 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 
     3721 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 
     3781 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 
     3841 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 
     3901 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 
     3961 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 
     4021 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 
     4081 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 
     4141 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 
     4201 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 
     4261 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 
     4321 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 
     4381 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 
     4441 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 
     4501 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 
     4561 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 
     4621 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 
     4681 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 
     4741 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 
     4801 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 
     4861 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 
     4921 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 
     4981 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 
     5041 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 
     5101 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 
     5161 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 
     5221 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 
     5281 aaagtgccac ctgggtcgac attgattatt gactagttat taatagtaat caattacggg 
     5341 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 
     5401 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 
     5461 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 
     5521 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga 
     5581 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg 
     5641 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg 
     5701 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta 
     5761 attattttgt gcagcgatgg gggcgggggg gggggggggg ccccccccag gcggggcggg 
     5821 gcggggcgag gggcggggcg gggcgaggcg gaaaggtgcg gcggcagcca atcagagcgg 
     5881 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg 
     5941 aagcgcgcgg cgggcgggag tcgttgcgcg ctgccttccc cccgtgcccc gctccgccgc 
     6001 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg 
     6061 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt 
     6121 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg 
     6181 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc 
     6241 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga 
     6301 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg 
     6361 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct 
     6421 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg 
     6481 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg 
     6541 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggaag gggcgcggcg 
     6601 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt 
     6661 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg 
     6721 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga 
     6781 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc 
     6841 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt 
     6901 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct 
     6961 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca 
     7021 aagaa 
//
