LOCUS pCAGGS-E-hTRAM 5527 bp DNA circular 24-OCT-2007 DEFINITION LMBP 5227. SOURCE ORGANISM COMMENT Accession number: LMBP 5227 (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium). Last modified: 24-OCT-2007 COMMENT VNTDATE|429710392| COMMENT VNTNAME|pCAGGS-E-hTRAM| COMMENT VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| COMMENT VNTOAUTHORTEL|0032-9-33-13-843| COMMENT VNTOAUTHOREML|bccm.genecorner@UGent.be| COMMENT VNTOAUTHORWWW|www.genecorner.ugent.be| COMMENT VNTOAUTHORAD1|Ghent University| COMMENT VNTOAUTHORAD2|Technologiepark 927| COMMENT VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| COMMENT VNTREPLTYPE|Plasmid COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pCAGGS-E-hTRAM" 0 5527 COMMENT (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) COMMENT (CObList) -1 "") COMMENT (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 COMMENT 0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) COMMENT (CWordArray) COMMENT (CStringList "PstI" "XhoI" "BalI" "BglII" "EcoRI" "HindII" "NotI") COMMENT (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 COMMENT "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 282 281 1 5527 0 0 0 0 0 0 0 0) (CStringList) COMMENT (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList COMMENT #0=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 783 0 1274 0 0 "") COMMENT #1=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 773 0 0 "") COMMENT #2=(CRSite (CStringList) "BalI" "tggcca" 4 0 0 0 "") COMMENT #3=(CRSite (CStringList) "BglII" "agatct" 2 0 2 178 0 813 0 0 "") COMMENT #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 4 0 0 "") COMMENT #5=(CRSite (CStringList) "HindII" "gtyrac" 4 0 2 547 0 3796 0 0 "") COMMENT #6=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "")) COMMENT (CObList COMMENT #7=(CFSignal (CObList) "E-tag" 22 0 0 16 54 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "16..54") COMMENT #8=(CFSignal (CObList) "hTRAM" 4 0 0 64 771 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "64..771") COMMENT #9=(CFSignal (CObList) "\337-globin polyA" 43 0 0 813 904 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "813..904") COMMENT #10=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 905 1261 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "905..1261") COMMENT #11=(CFSignal (CObList) "lac promoter" 30 0 1 1339 1421 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(1339..1421)") COMMENT #12=(CFSignal (CObList) "SV40 ori" 33 0 0 1563 1638 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1563..1638") COMMENT #13=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1669 1743 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "1669..1743") COMMENT #14=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1669 1743 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(1669..1743)") COMMENT #15=(CFSignal (CObList) "pMB1 ori" 33 0 1 1858 2259 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(1858..2259)") COMMENT #16=(CFSignal (CObList) "ampicillin" 4 0 1 2800 3660 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(2800..3660)") COMMENT #17=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 3796 4173 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "3796..4173") COMMENT #18=(CFSignal (CObList) "AG promoter" 29 0 0 4179 5524 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "4179..5524") COMMENT #19=(CFSignal (CObList) "intron" 15 0 0 4548 5476 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "4548..5476")) (CObList) (CObList) (CObList) COMMENT (CObList) COMMENT (CObList COMMENT #20=(CFSignal (CObList) "LMBP 5227" 2 0 0 0 0 0 (CStringList) COMMENT (CStringList) 1 1 0 0 "") COMMENT #21=(CFSignal (CObList) "Last modified: 24-OCT-2007" 2 0 0 0 0 0 COMMENT (CStringList) (CStringList) 1 1 0 0 "")) (CObList) COMMENT (CTextView 0 COMMENT #22=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #23=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pCAGGS-E-hTRAM" 2) COMMENT 5 "" 0 4) COMMENT #24=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA Plasmid 'pCAGGS-E-hTRAM'" 1) COMMENT #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 5227." 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#289=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -938 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 COMMENT (CStringList) 0 0 (CObList))) FEATURES Location/Qualifiers misc_marker 16..54 /vntifkey="22" /label=E-tag /note="E-tag" CDS 64..771 /vntifkey="4" /label=hTRAM /note="human TRIF-related adapter molecule coding sequence" terminator 813..904 /vntifkey="43" /label=ß-globin\polyA /note="3' UTR of the rabbit ß-globin gene, incl. polyA" misc_feature 905..1261 /vntifkey="21" /label=3'\FR\of\ß-globin /note="3' flanking region of the rabbit ß-globin gene" promoter complement(1339..1421) /vntifkey="30" /label=lac\promoter /note="E. coli lac operon promoter" rep_origin 1563..1638 /vntifkey="33" /label=SV40\ori /note="bidirectional origin of SV40" terminator 1669..1743 /vntifkey="43" /label=SV40\polyA\early /note="polyA signal of the SV40 early region" terminator complement(1669..1743) /vntifkey="43" /label=SV40\polyA\late /note="polyA signal of the SV40 late region" rep_origin complement(1858..2259) /vntifkey="33" /label=pMB1\ori /note="origin plasmid" CDS complement(2800..3660) /vntifkey="4" /label=ampicillin /note="antibiotic resistance gene; coding sequence" promoter 3796..4173 /vntifkey="29" /label=hCMV-IE\enhancer /note="human CMV-IE enhancer" promoter 4179..5524 /vntifkey="29" /label=AG\promoter /note="chicken ß-actin/rabbit ß-globin hybrid promoter" intron 4548..5476 /vntifkey="15" /label=intron /note="chicken ß-actin/rabbit ß-globin hybrid intron" BASE COUNT 1236 a 1425 c 1501 g 1365 t ORIGIN 1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 61 gcaatgggta tcgggaagtc taaaataaat tcctgccctc tttctctctc ttggggtaaa 121 aggcacagtg tggatacaag tccaggatat catgagtcag attccaagaa gtctgaagat 181 ctatccttgt gtaatgttgc tgagcacagc aatacaacag aggggccaac aggaaagcag 241 gagggagctc agagcgtgga agagatgttt gaagaagaag ctgaagaaga ggtgttcctc 301 aaatttgtga tattgcatgc agaagatgac acagatgaag ccctcagagt ccagaatctg 361 ctacaagatg actttggtat caaacccgga ataatctttg ctgagatgcc atgtggcaga 421 cagcatttac agaatttaga tgatgctgta aatgggtctg catggacaat cttattactg 481 actgaaaact ttttaagaga tacttggtgt aatttccagt tctatacgtc cctaatgaac 541 tccgttaaca ggcagcataa atacaactct gttataccca tgcggcccct gaacaatccc 601 cttccccgag aaaggactcc ctttgccctc caaaccatca atgccttaga ggaagaaagt 661 cgtggatttc ctacacaagt agaaagaatt tttcaggagt ctgtgtataa gacacaacaa 721 actatatgga aagagacaag aaatatggta caaagacaat ttattgcctg actcgagctg 781 cagccaatgc cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg 841 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca 901 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa 961 atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc 1021 tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg 1081 ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt 1141 gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca 1201 gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat 1261 ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat 1321 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 1381 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 1441 cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc 1501 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 1561 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 1621 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt 1681 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 1741 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 1801 atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 1861 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 1921 cagctcactc aaaggcggta atacggttat ccacagaatc agggataacg caggaaagaa 1981 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 2041 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 2101 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 2161 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 2221 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 2281 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 2341 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 2401 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 2461 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 2521 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 2581 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 2641 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 2701 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 2761 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 2821 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 2881 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 2941 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 3001 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 3061 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 3121 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 3181 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 3241 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 3301 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 3361 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 3421 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 3481 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 3541 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 3601 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 3661 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 3721 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 3781 agtgccacct gggtcgacat tgattattga ctagttatta atagtaatca attacggggt 3841 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc 3901 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag 3961 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc 4021 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg 4081 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc 4141 agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct 4201 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt attttttaat 4261 tattttgtgc agcgatgggg gcgggggggg ggggggggcc ccccccaggc ggggcggggc 4321 ggggcgaggg gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg 4381 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa 4441 gcgcgcggcg ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc tccgccgccg 4501 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg 4561 acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc 4621 tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct 4681 cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg 4741 gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg 4801 ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct 4861 gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc 4921 aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc 4981 tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg 5041 tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggaaggg gcgcggcggc 5101 ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa 5161 tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg 5221 aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag 5281 gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag 5341 cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc 5401 ggcttctggc gtgtgaccgg cggctctagc tagagcctct gctaaccatg ttcatgcctt 5461 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg 5521 caaagaa //