ID pCAGGS-E-hTRAM standard; circular DNA; ; 5527 BP. DE LMBP 5227. CC Accession number: LMBP 5227 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium). CC Last modified: 24-OCT-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_marker 16..54 FT /vntifkey="22" FT /label=E-tag FT /note="E-tag" FT CDS 64..771 FT /vntifkey="4" FT /label=hTRAM FT /note="human TRIF-related adapter molecule coding sequence" FT terminator 813..904 FT /vntifkey="43" FT /label=ß-globin\polyA FT /note="3' UTR of the rabbit ß-globin gene, incl. polyA" FT misc_feature 905..1261 FT /vntifkey="21" FT /label=3'\FR\of\ß-globin FT /note="3' flanking region of the rabbit ß-globin gene" FT promoter complement(1339..1421) FT /vntifkey="30" FT /label=lac\promoter FT /note="E. coli lac operon promoter" FT rep_origin 1563..1638 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT terminator 1669..1743 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(1669..1743) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT rep_origin complement(1858..2259) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(2800..3660) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" FT promoter 3796..4173 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT /note="human CMV-IE enhancer" FT promoter 4179..5524 FT /vntifkey="29" FT /label=AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid promoter" FT intron 4548..5476 FT /vntifkey="15" FT /label=intron FT /note="chicken ß-actin/rabbit ß-globin hybrid intron" SQ Sequence 5527 BP; 1236 A; 1425 C; 1501 G; 1365 t; ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 60 gcaatgggta tcgggaagtc taaaataaat tcctgccctc tttctctctc ttggggtaaa 120 aggcacagtg tggatacaag tccaggatat catgagtcag attccaagaa gtctgaagat 180 ctatccttgt gtaatgttgc tgagcacagc aatacaacag aggggccaac aggaaagcag 240 gagggagctc agagcgtgga agagatgttt gaagaagaag ctgaagaaga ggtgttcctc 300 aaatttgtga tattgcatgc agaagatgac acagatgaag ccctcagagt ccagaatctg 360 ctacaagatg actttggtat caaacccgga ataatctttg ctgagatgcc atgtggcaga 420 cagcatttac agaatttaga tgatgctgta aatgggtctg catggacaat cttattactg 480 actgaaaact ttttaagaga tacttggtgt aatttccagt tctatacgtc cctaatgaac 540 tccgttaaca ggcagcataa atacaactct gttataccca tgcggcccct gaacaatccc 600 cttccccgag aaaggactcc ctttgccctc caaaccatca atgccttaga ggaagaaagt 660 cgtggatttc ctacacaagt agaaagaatt tttcaggagt ctgtgtataa gacacaacaa 720 actatatgga aagagacaag aaatatggta caaagacaat ttattgcctg actcgagctg 780 cagccaatgc cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg 840 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca 900 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa 960 atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc 1020 tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg 1080 ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt 1140 gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca 1200 gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat 1260 ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat 1320 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 1380 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 1440 cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc 1500 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 1560 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 1620 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt 1680 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 1740 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 1800 atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 1860 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 1920 cagctcactc aaaggcggta atacggttat ccacagaatc agggataacg caggaaagaa 1980 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 2040 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 2100 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 2160 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 2220 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 2280 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 2340 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 2400 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 2460 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 2520 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 2580 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 2640 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 2700 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 2760 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 2820 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 2880 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 2940 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 3000 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 3060 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 3120 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 3180 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 3240 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 3300 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 3360 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 3420 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 3480 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 3540 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 3600 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 3660 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 3720 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 3780 agtgccacct gggtcgacat tgattattga ctagttatta atagtaatca attacggggt 3840 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc 3900 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag 3960 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc 4020 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg 4080 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc 4140 agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct 4200 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt attttttaat 4260 tattttgtgc agcgatgggg gcgggggggg ggggggggcc ccccccaggc ggggcggggc 4320 ggggcgaggg gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg 4380 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa 4440 gcgcgcggcg ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc tccgccgccg 4500 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg 4560 acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc 4620 tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct 4680 cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg 4740 gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg 4800 ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct 4860 gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc 4920 aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc 4980 tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg 5040 tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggaaggg gcgcggcggc 5100 ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa 5160 tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg 5220 aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag 5280 gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag 5340 cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc 5400 ggcttctggc gtgtgaccgg cggctctagc tagagcctct gctaaccatg ttcatgcctt 5460 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg 5520 caaagaa 5527 //