LOCUS       pCAGGS-E-IDmMyD88        5005 bp    DNA     circular     23-FEB-2007
DEFINITION  LMBP 5228.
SOURCE      
  ORGANISM  
COMMENT     Accession number:  5228
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
COMMENT     VNTDATE|438882238|
COMMENT     VNTNAME|pCAGGS-E-IDmMyD88|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-E-IDmMyD88" 0 5005 
COMMENT       (CDBMol 0 0 2 1 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList 
COMMENT         #0=(CFragment 3 1 0 2573 0 "" 0 "" "" "" "" "" "" "PCR-IDmMyd88-Eli" "" 
COMMENT             0 0 1 146 4860 5005 13 158 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "NotI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "XhoI" "" 1 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 3 1 0 13568 0 "" 0 "" "" "" "" "" "" "pCAGGSEhA20" "" 0 0 
COMMENT             1 4859 1 4859 2476 56 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "XhoI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "NotI" "" 1 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) (CStringList "NotI" "XhoI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 3076 3453 1 5005 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #2=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 4860 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 1 0 0 "")) 
COMMENT       (CObList 
COMMENT        #4=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 185 541 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "185..541") 
COMMENT        #5=(CFSignal (CObList) "3' UTR of the \337-globin, incl. polyA" 43 0 0 
COMMENT            93 184 0 (CStringList) (CStringList) 1 1 1 1 "93..184") 
COMMENT        #6=(CFSignal (CObList) "lac promoter" 30 0 1 619 701 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(619..701)") 
COMMENT        #7=(CFSignal (CObList) "SV40 ori" 33 0 0 748 948 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "748..948") 
COMMENT        #8=(CFSignal (CObList) "SV40 polyA early" 43 0 0 949 1023 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "949..1023") 
COMMENT        #9=(CFSignal (CObList) "SV40 polyA late" 43 0 1 949 1023 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(949..1023)") 
COMMENT        #10=(CFSignal (CObList) "pMB1 ori" 33 0 1 1138 1539 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1138..1539)") 
COMMENT        #11=(CFSignal (CObList) "ampicillin" 4 0 1 2080 2940 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2080..2940)") 
COMMENT        #12=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 3076 3453 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3076..3453") 
COMMENT        #13=(CFSignal (CObList) "E-tag" 22 0 0 4819 4857 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4819..4857") 
COMMENT        #14=(CFSignal (CObList) "ID-domain of mMyD88" 4 0 0 4867 5004 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4867..5004") 
COMMENT        #15=(CFSignal (CObList) "AG promoter" 29 0 0 3459 4803 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3459..4803")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList 
COMMENT        #16=(CFSignal (CObList) "Coll. n\260: 5228" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #17=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #18=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) 
COMMENT                    "pCAGGS-E-IDmMyD88" 2) 5 "" 0 4) 
COMMENT              #19=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pCAGGS-E-IDmMyD88'" 1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Collection number: 5228" 1) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 02/23/07 03:43" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 02/23/07 03:51" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5005 bp" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Constructed" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #30=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #31=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #32=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #33=(CLinePar (CParagraph 0 (15 #16# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Coll. n\260: 5228"))) "Annotations") 
COMMENT              #34=(CFolderPar 
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COMMENT                    (CObjectList 
COMMENT                     #35=(CFolderPar 
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COMMENT                           (CObjectList 
COMMENT                            #36=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "parent position: from 13 to 158" 1) 
COMMENT                            #37=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "original length: 146" 1) 
COMMENT                            #38=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "molecule position: from 4860 to 5005" 1) 
COMMENT                            #39=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) 
COMMENT                                  (CObjectList 
COMMENT                                   #40=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  NotI site #1" 1))) "Left Terminus") 
COMMENT                            #41=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) 
COMMENT                                  (CObjectList 
COMMENT                                   #42=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  XhoI site #1" 1))) "Right Terminus"))) 
COMMENT                          "#1: FRAGMENT of <a href=\"vnti:dnarna/PCR-IDmMyd88-Eli?sel=13-158\">PCR-IDmMyd88-Eli</a> ") 
COMMENT                     #43=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 0 (5 #1# 0) 1 2 1 0 195) 
COMMENT                           (CObjectList 
COMMENT                            #44=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "parent position: from 2476 to 56" 1) 
COMMENT                            #45=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "original length: 4859" 1) 
COMMENT                            #46=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "molecule position: from 1 to 4859" 1) 
COMMENT                            #47=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  XhoI site #1" 1))) "Left Terminus") 
COMMENT                            #49=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) 
COMMENT                                  (CObjectList 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  NotI site #1" 1))) "Right Terminus"))) 
COMMENT                          "#2: FRAGMENT of <a href=\"vnti:dnarna/pCAGGSEhA20?sel=2476-56\">pCAGGSEhA20</a> "))) 
COMMENT                   "Component Fragments") 
COMMENT              #51=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #52=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #53=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2080 (3 #11# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2080  End: 2940 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene" 1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2080..2940)" 1))) 
COMMENT                                 "ampicillin") 
COMMENT                            #58=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4867  End: 5004" 1) 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4867..5004" 1))) 
COMMENT                                 "ID-domain of mMyD88"))) "CDS (2 total)") 
COMMENT                     #62=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #63=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 185 (3 #4# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 185   End: 541 " 1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "3' flanking region of the rabbit \347-globin gene" 
COMMENT                                        1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   185..541" 1))) 
COMMENT                                 "3' FR of \337-globin"))) 
COMMENT                          "Misc. Feature (1 total)") 
COMMENT                     #68=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #69=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4819 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4819  End: 4857" 1) 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) "E-tag" 
COMMENT                                        1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4819..4857" 1))) "E-tag"))) 
COMMENT                          "Misc. Marker (1 total)") 
COMMENT                     #74=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 2 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #75=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3076 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3076  End: 3453" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "hCMV-IE enhancer" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3076..3453" 1))) "hCMV-IE enhancer") 
COMMENT                            #80=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3459 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3459  End: 4803" 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "\347-actin/\347-globin hybrid promoter" 
COMMENT                                        1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3459..4803" 1))) "AG promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #85=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #86=(CFolderPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 619   End: 701  (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "E. coli lac operon promoter" 1) 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(619..701)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
COMMENT                     #91=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #92=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 748   End: 948 " 1) 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "SV40 fragment containing bidirectional ori" 
COMMENT                                        1) 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   748..948" 1))) "SV40 ori") 
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COMMENT                                        "Start: 1138  End: 1539 (Complementary)" 
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COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "origin plasmid" 1) 
COMMENT                                   #100=(CLinePar 
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COMMENT                                         "Original Location Description:" 1) 
COMMENT                                   #101=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   complement(1138..1539)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (2 total)") 
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COMMENT                                           "Start: 93    End: 184 " 1) 
COMMENT                                     #105=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "3' UTR of the rabbit \347-globin gene, incl. polyA" 
COMMENT                                           1) 
COMMENT                                     #106=(CLinePar 
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COMMENT                                           "Original Location Description:" 1) 
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COMMENT                                           "   93..184" 1))) 
COMMENT                                   "3' UTR of the \337-globin, incl. polyA") 
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COMMENT                                           "Start: 949   End: 1023" 1) 
COMMENT                                     #110=(CLinePar 
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COMMENT                                           "polyA signal of the SV40 early region" 
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COMMENT                                           "   949..1023" 1))) 
COMMENT                                   "SV40 polyA early") 
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COMMENT                                     #115=(CLinePar 
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COMMENT                                           "polyA signal of the SV40 late region" 
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COMMENT                                           "   complement(949..1023)" 1))) 
COMMENT                                   "SV40 polyA late"))) "Terminator (3 total)"))) 
COMMENT                   "Feature Map") 
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COMMENT                                            " N1: 4860 " 1))))) "NotI: 1 site") 
COMMENT                            2 6 "GCGGCCGC" "CGCCGGCG") 
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COMMENT                                            " N1: 1 " 1))))) "XhoI: 1 site") 1 5 
COMMENT                            "CTCGAG" "GAGCTC"))) "Restriction/Methylation Map")))) 
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COMMENT          #126=(CWidgetStyle "Mark 1" 0 0 1 
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COMMENT          #127=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.64 0 1 -1 
COMMENT                "@N (@H%)" 0) 
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COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
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COMMENT                "@N" 0) 
COMMENT          #130=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
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FEATURES             Location/Qualifiers
     CDS             4867..5004
                     /vntifkey="4"
                     /label=ID-domain\of\mMyD88
     misc_marker     4819..4857
                     /vntifkey="22"
                     /label=E-tag
                     /note="E-tag"
     terminator      93..184
                     /vntifkey="43"
                     /label=3'\UTR\of\the\ß-globin,\incl.\polyA
                     /note="3' UTR of the rabbit ç-globin gene, incl. polyA"
     misc_feature    185..541
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit ç-globin gene"
     promoter        complement(619..701)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     rep_origin      748..948
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="SV40 fragment containing bidirectional ori"
     terminator      949..1023
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(949..1023)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(1138..1539)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(2080..2940)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene"
     promoter        3076..3453
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="hCMV-IE enhancer"
     promoter        3459..4803
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="ç-actin/ç-globin hybrid promoter"
BASE COUNT     1057 a      1329 c      1400 g      1219 t 
ORIGIN
        1 tcgaggtcga ggaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg 
       61 tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg 
      121 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca 
      181 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa 
      241 atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc 
      301 tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg 
      361 ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt 
      421 gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca 
      481 gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat 
      541 ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat 
      601 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 
      661 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 
      721 cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc 
      781 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 
      841 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 
      901 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt 
      961 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 
     1021 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 
     1081 atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 
     1141 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 
     1201 cagctcactc aaaggcggta atacggttat ccacagaatc agggataacg caggaaagaa 
     1261 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 
     1321 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 
     1381 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 
     1441 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 
     1501 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 
     1561 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 
     1621 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 
     1681 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 
     1741 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 
     1801 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 
     1861 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 
     1921 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 
     1981 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 
     2041 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 
     2101 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 
     2161 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 
     2221 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 
     2281 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 
     2341 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 
     2401 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 
     2461 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 
     2521 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 
     2581 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 
     2641 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 
     2701 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 
     2761 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 
     2821 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 
     2881 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 
     2941 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 
     3001 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 
     3061 agtgccacct gggtcgacat tgattattga ctagttatta atagtaatca attacggggt 
     3121 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc 
     3181 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag 
     3241 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc 
     3301 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg 
     3361 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc 
     3421 agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct 
     3481 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt attttttaat 
     3541 tattttgtgc agcgatgggg gcgggggggg ggggggggcc ccccccaggc ggggcggggc 
     3601 ggggcgaggg gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg 
     3661 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa 
     3721 gcgcgcggcg ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc tccgccgccg 
     3781 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg 
     3841 acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc 
     3901 tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct 
     3961 cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg 
     4021 gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg 
     4081 ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct 
     4141 gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc 
     4201 aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc 
     4261 tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg 
     4321 tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggaaggg gcgcggcggc 
     4381 ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa 
     4441 tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg 
     4501 aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag 
     4561 gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag 
     4621 cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc 
     4681 ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc 
     4741 tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa 
     4801 gaattgaatt ccaccatggg tgcgccggtg ccgtatccgg acccgctgga accgcgtgcg 
     4861 gccgccgagg aggactgcca gaaatactta ggtaagcagc agaaccagga gtccgagaag 
     4921 cctttacagg tggccagagt ggaaagcagt gtcccacaaa caaaggaact gggaggcatc 
     4981 accacccttg atgaccccct atgac 
//
